1.Most enzymatic reactions consume substrate or produce products which cannot be monitored directly with spectrophotometric methods because they do not absorb light. What can be done in this case to monitor the substrate consumption or product formation in order to calculate kinetic parameters? switch to a continuous assay switch to a discontinuous assay use a coupled reaction which converts the product into one that has a chromophore All of the above

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
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Chapter1: Biochemistry: An Evolving Science
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1.Most enzymatic reactions consume
substrate or produce products which
cannot be monitored directly with
spectrophotometric methods because they
do not absorb light. What can be done in
this case to monitor the substrate
consumption or product formation in order
to calculate kinetic parameters?
switch to a continuous assay
switch to a discontinuous assay
use a coupled reaction which converts the
product into one that has a chromophore
All of the above
Transcribed Image Text:1.Most enzymatic reactions consume substrate or produce products which cannot be monitored directly with spectrophotometric methods because they do not absorb light. What can be done in this case to monitor the substrate consumption or product formation in order to calculate kinetic parameters? switch to a continuous assay switch to a discontinuous assay use a coupled reaction which converts the product into one that has a chromophore All of the above
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