16. Why is re-circularized insert generally not a problem in cloning procedures, while re- circularized vector can be a problem? Is it because re-circularized insert: A: is detrimental to bacteria B: is a kind of product that DNA ligase cannot form C: makes E. coli sensitive to antibiotics D: lacks a replication origin E: is beneficial to bacteria om

16. Why is re-circularized insert generally not a problem in cloning procedures, while re- circularized vector can be a problem? Is it because re-circularized insert: A: is detrimental to bacteria B: is a kind of product that DNA ligase cannot form C: makes E. coli sensitive to antibiotics D: lacks a replication origin E: is beneficial to bacteria om

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter18: Dna Technologies: Making And Using Genetically Altered Organisms, And Other Applications

Section: Chapter Questions

Problem 3TYK: Why are antibiotic resistance markers such as ampR important components of bacterial plasmid cloning...

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Which of the following is NOT true about transduction? The bacteriophage's own viral DNA is the source of new genes for bacterial recombination Pieces of donor bacterial DNA can be inserted into the recipient bacterial cell's chromosome None of the other four answers (All are true statements about transduction) O Genetic recombination can result from bacteriophage (phage) virus infection of bacteria During the assembly of new phage particles, pieces of bacterial DNA are sometimes mistakenly inserted into new virus particles instead of phage (viral) DNA
1. Choose the phrase from the right column that best fitsthe term in the left column.a. transformation 1. requires supplements in medium forgrowthb. conjugation 2. a method for mutagenizing genes inbacterial genomesc. transduction 3. small circular DNA molecule that canintegrate into the chromosomed. lytic cycle 4. the core genes that define a bacterialspecies plus all of the genes unique toindividual strainse. lysogeny 5. transfer of DNA requiring directphysical contactf. episome 6. integration of phage DNA into thechromosomeg. auxotroph 7. infection by phages in which lysis ofcells releases new virus particlesh. pangenome 8. transfer of naked DNAi. gene targeting 9. transfer of DNA between bacteria viavirus particles
1) Follow the steps involved in DNA or gene cloning 2) In what process do bacteria take up the recombinant plasmid DNA 3) What are the advantages or replications of gene cloning?
5) Below is an image that shows both reproductive and therapeutic cloning. Use this image to answer compare and contrast therapeutic and reproductive cloning. Are they used for similar means…etc. Once you have done that answer the question below. a) There are two types of therapeutic cloning. What are they and how are they different?
WHAT is the subject of the figure above? WHAT is the main message of the figure? Give a BRIEF but complete explanation of the role of each of the sections depicted.
16. Which of the following enzyme repairs the DNA backbone during molecular cloning (that is you are inserting the DNA fragment into a plasmid and the bases in the sticky ends are aligned but the backbone needs to be repaired). a) reverse transcriptase b) restriction enzymes c) DNA ligase d) polymerase
Part 1. Gene Cloning 1) How is a gene inserted into a plasmid cloning vector? Be specific, but be brief. No more than 2 (non-run-on) sentences please, and do not exceed this space. 2) A geneticist has transformed a vector with an ampicillin resistance gene into E. coli cells and plated those cells on media containing ampicillin. She comes back 24 hours later and sees only blue colonies on the media. a) How does she know that the cells growing on the media were successfully transformed with the vector? b) What does it mean that the colonies are blue?
When an E. coli donor cell duplicates a strand of plasmid DNA, and passes this DNA strand to a recipient E. coli cell, without the use of naked DNA in solution or of a viral vector, this is: an example of horizontal gene transfer by means of lysogenic bacteriophages an example of horizontal gene transfer by means of lytic bacteriophages an example of horizontal gene transfer by means of transformation an example of horizontal gene transfer by means of transduction an example of horizontal gene transfer by means of conjugation
All of the following lead to horizontal gene transfer EXCEPT. O plasmids transfer of genetic material from parent to progeny transposable elements viral infection 0000
The idea behind PCR-based diagnostics is that a very small number of microbial genomes in a patient sample can be multiplied by PCR and more easily detected by the clinical team managing the patient’s care. Also, genetic-based diagnostics are very useful for viral infections because we don’t have biochemical tests, etc. to distinguish one virus from another (remember, viruses are metabolically inactive). However, a lot of work goes into the development of these tests. For instance, PCR requires primers that are complementary to the viral genome that is being copied. If primers are complementary to the target genome, what must scientists know to design primers that bind to the viral genome to be copied? (I mean this to be a general question; don’t look up the details of designing primers)
Gene cloning vectors should have the property of self-replication so that DNA insert can be copied as the vector replicates. Lütfen birini seçin: O a. True O b. False
A _______ is a molecule that can be used to carry a fragment of DNA into a host organism. a. cloning vector c. GMO b. chromosome d. cDNA