17. Which pair of scientists discovered the structure of the DNA double helix and helped to make recombinant DNA technology possible?

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
icon
Related questions
icon
Concept explainers
Question
17. Which pair of scientists discovered the structure of the DNA double helix and helped to make
recombinant DNA technology possible?
18. In this experiment, which of the following DNA molecules carries the gene for kanamycin resistance?
A) the TN903 transposon
B) the pUC plasmid
19. In this experiment, which of the following DNA molecules carries the gene for ampicillin resistance?
A) the TN903 transposon
B) the pUC plasmid
20. In this experiment, both the transposon DNA and the plasmid DNA are cut with which of the
following restriction enzymes?
А) НаеШ
В) ЕcoRI
C) SacI
D) PvuII
21. The restriction enzyme used to cut the DNA generates:
A) blunt ends
B) sticky ends
22. In this experiment, what is required to join the transposon fragment with the linearized plasmid?
A) the enzyme DNA ligase
B) a buffer solution containing ATP
C) an incubator set within the temperature range of 4-22°C
D) all of the above
23. What is the purpose of treating the linearized plasmid with the enzyme alkaline phosphatase?
A) to prevent any unwanted ligation products
B) to provide the energy needed for the ligation reaction
C) to maintain an alkaline pH for the ligation reaction
D) all of the above
24. The initial E. coli strain (before transformation) used in this experiment is:
A) ampicillin-resistant
B) kanamycin-resistant
C) ampicillin-resistant and kanamycin-resistant
D) neither ampicillin-resistant nor kanamycin-resistant
25. In this experiment, a bacterial "lawn" would result from plating bacterial cells onto which of the
following types of agar growth media?
A) media containing ampicillin
B) media containing kanamycin
C) media containing both ampicillin and kanamycin
D) media containing neither ampicillin nor kanamycin
Transcribed Image Text:17. Which pair of scientists discovered the structure of the DNA double helix and helped to make recombinant DNA technology possible? 18. In this experiment, which of the following DNA molecules carries the gene for kanamycin resistance? A) the TN903 transposon B) the pUC plasmid 19. In this experiment, which of the following DNA molecules carries the gene for ampicillin resistance? A) the TN903 transposon B) the pUC plasmid 20. In this experiment, both the transposon DNA and the plasmid DNA are cut with which of the following restriction enzymes? А) НаеШ В) ЕcoRI C) SacI D) PvuII 21. The restriction enzyme used to cut the DNA generates: A) blunt ends B) sticky ends 22. In this experiment, what is required to join the transposon fragment with the linearized plasmid? A) the enzyme DNA ligase B) a buffer solution containing ATP C) an incubator set within the temperature range of 4-22°C D) all of the above 23. What is the purpose of treating the linearized plasmid with the enzyme alkaline phosphatase? A) to prevent any unwanted ligation products B) to provide the energy needed for the ligation reaction C) to maintain an alkaline pH for the ligation reaction D) all of the above 24. The initial E. coli strain (before transformation) used in this experiment is: A) ampicillin-resistant B) kanamycin-resistant C) ampicillin-resistant and kanamycin-resistant D) neither ampicillin-resistant nor kanamycin-resistant 25. In this experiment, a bacterial "lawn" would result from plating bacterial cells onto which of the following types of agar growth media? A) media containing ampicillin B) media containing kanamycin C) media containing both ampicillin and kanamycin D) media containing neither ampicillin nor kanamycin
Expert Solution
steps

Step by step

Solved in 2 steps

Blurred answer
Knowledge Booster
Molecular techniques
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:
9780815344322
Author:
Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:
W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:
9781260159363
Author:
Martin, Terry R., Prentice-craver, Cynthia
Publisher:
McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Inquiry Into Life (16th Edition)
Biology
ISBN:
9781260231700
Author:
Sylvia S. Mader, Michael Windelspecht
Publisher:
McGraw Hill Education