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10. The amino terminus of a wildtype enzyme in yeast has the amino acid sequence Met-Leu-His-Tyr-Met-Gly-Asp-Tyr-Pro A mutant, X, is found that contains an inactive form of the enzyme with the sequence 1_12_2021 Met-Gly-Asp-Tyr-Pro at the amino terminus and a wildtype sequence at the carboxyl terminus. A second mutant, Y, also lacks enzyme activity, but a full-length protein is not observed. Instead, mutant Y makes a short peptide containing just 3 amino acids. What mutations could account for these changes in mutant X and mutant Y? As part of your answer, show the sequences of the genes encoding the wildtype proteins and the 2 mutant proteins, and indicate in the wild type sequence where the mutations that gave rise to X and Y actually occurred. What is the sequence of the tripeptide produced by mutant Y? 4

10. The amino terminus of a wildtype enzyme in yeast has the amino acid sequence Met-Leu-His-Tyr-Met-Gly-Asp-Tyr-Pro A mutant, X, is found that contains an inactive form of the enzyme with the sequence 1_12_2021 Met-Gly-Asp-Tyr-Pro at the amino terminus and a wildtype sequence at the carboxyl terminus. A second mutant, Y, also lacks enzyme activity, but a full-length protein is not observed. Instead, mutant Y makes a short peptide containing just 3 amino acids. What mutations could account for these changes in mutant X and mutant Y? As part of your answer, show the sequences of the genes encoding the wildtype proteins and the 2 mutant proteins, and indicate in the wild type sequence where the mutations that gave rise to X and Y actually occurred. What is the sequence of the tripeptide produced by mutant Y? 4

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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**Transcription for Educational Website:** --- **10. Analysis of Enzyme Mutation in Yeast:** The amino terminus of a wildtype enzyme in yeast has the following amino acid sequence: **Met-Leu-His-Tyr-Met-Gly-Asp-Tyr-Pro** A mutant, X, has been identified with an inactive form of the enzyme marked by the sequence: **Met-Gly-Asp-Tyr-Pro** This sequence is present at the amino terminus, while the carboxyl terminus remains wildtype. A second mutant, Y, also lacks enzyme activity, and no full-length protein is observed. Instead, mutant Y produces a short peptide containing only 3 amino acids. **Questions for Analysis:** 1. **Mutational Analysis:** What genetic mutations could explain the alterations seen in mutant X and mutant Y? 2. **Gene Sequencing:** Demonstrate the sequences of the genes encoding the wildtype proteins and the two mutant proteins. 3. **Mutation Identification:** Indicate the specific wildtype sequence mutations that resulted in mutants X and Y. 4. **Trimer Peptide Identification:** What is the amino acid sequence of the tripeptide produced by mutant Y? --- **Note:** There are no graphs or diagrams associated with this text.
Transcribed Image Text:**Transcription for Educational Website:** --- **10. Analysis of Enzyme Mutation in Yeast:** The amino terminus of a wildtype enzyme in yeast has the following amino acid sequence: **Met-Leu-His-Tyr-Met-Gly-Asp-Tyr-Pro** A mutant, X, has been identified with an inactive form of the enzyme marked by the sequence: **Met-Gly-Asp-Tyr-Pro** This sequence is present at the amino terminus, while the carboxyl terminus remains wildtype. A second mutant, Y, also lacks enzyme activity, and no full-length protein is observed. Instead, mutant Y produces a short peptide containing only 3 amino acids. **Questions for Analysis:** 1. **Mutational Analysis:** What genetic mutations could explain the alterations seen in mutant X and mutant Y? 2. **Gene Sequencing:** Demonstrate the sequences of the genes encoding the wildtype proteins and the two mutant proteins. 3. **Mutation Identification:** Indicate the specific wildtype sequence mutations that resulted in mutants X and Y. 4. **Trimer Peptide Identification:** What is the amino acid sequence of the tripeptide produced by mutant Y? --- **Note:** There are no graphs or diagrams associated with this text.
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