10. Examine this image of a sample that was streaked using the quadrant streakisolation technique. Answer the questions below. (Please see color image in theelectronic version of this document in our Lab Week 4 folder. You may want to alsoincrease the magnification of the image too.) (50 pts each, 100 pts total)Harold BensonImage Description: Quadrant streakon Nutrient Agar. Examine theimage for colonial morphologyobservations.a. Is this an example of a PURE culture or a MIXED culture?b. Briefly explain how you can support your answer in part a of this question.

Detailed Analysis of a Mixed Culture on Nutrient AgarA mixed culture, as seen in the image provided,…

Answered: 10. Examine this image of a sample that…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Similar questions

Based only on your EMB and MSA results, build a dichotomous key that could differentiate between these four strains.
Answer and the image below and provide explanations.
Isolate #2: Cell Morphology (shape) . a. b. Isolate #2: Gram stain result Gram Postive. (The color I see on my screen is purple.) c. List at least two genera (not species) of bacteria that are consistent with the Gram reaction and morphology you observed. d. Are any of these genera are normal flora on the skin? If so which one(s)? (1 pt) ***Please answer the four questions base on the picture provided.***
What type of medium is best used to maintain a preserve specimen before clinical analysis? A. Enriched b. Selective c. Differential d. Reducing e. Nutrient
1.What advantage(s) does the pour plate method have over the streak-plate method? 2.Why is the loop flamed before it is placed in a culture tube? Why is it flamed after completing the inoculation? 3.Before inoculating and pouring molten nutrient agar into a plate, why must the agar first be cooled to 50° C? 4.Explain why plates should be inverted during incubation. 5.Explain why plates should be inverted during incubation.
Describe characteristics of Proteus Vulgaris in the Agar: (How does colonies look like (color) and explain does it grow on that agar. Blood Agar (Aerobic) MacConkey EMB PEA Mannitol Salt Agar Chocolate Agar Nutrient Agar
1. You were given a mixed nutrient agar broth culture of bacteria. a. How will you determine the different types of bacteria present in the mixed culture? b. How will you make a pure culture of these bacteria in a slant nutrient agar? c. How will you identify these bacteria?
Describe the results for each test indicated below. Use full sentences and write your answers in proper paragraph form. Gram stain Positive or Negative (How did you know?) Cell arrangement Cell morpholog Phenol Red Fermentation Did fermentation occur in each sugar? What products were produced? (How could you tell?) Streak for Isolation Successful or unsuccessful? (How could you tell?) Pigmented or non-pigmented? describe any other characteristics of Colony Morphology
a.Describe what makes thioglycollate medium suitable for culturing anaerobes. What would the growth patterns of Clostridium sporogenes and Micrococcus luteus be in this medium? b. In the Kligler test, why do we inoculate the surface of the agar slope and then stab into the butt of the slope? What does a pink coloured colony indicate when using MAC (MacConkey Agar)?
LAB SECTION I WAS PRESENT AND PERFORMED THIS EXERCISE (INITIALS) The Lethal Effect of Ultraviolet Radiation on Microbial Growth OBSERVATIONS AND INTERPRETATIONS Enter your class data below. Score the relative amount of growth on each plate. Use the numbers 0, 1, 2, or 3 (0 is no growth; 3 is abundant growth). Organism B. subtilis 5 seconds Imin 5+ 1 3 minutes 10 minutes 10 seconds 5+ 3min 30 seconds 5min 4+ minute 10min S. aureus 5 + 3 + 4 니- + 2 1 Micrococcus 1 10 sec 4 M 31m 10min 3'ın Questions of this exercise is to demonstrate the comparative effect of UV on two bacterial populations. The purpose This could have been accomplished without the cardboard cover. Why was the cover used? control site 1 Expe SECTION TWO MICROBIAL GROWTH DATA SHEET 2-1
1. how does colony appearance and location with respect to the agar differ on the spread and pour plate? 2. list two additional factors that contribute to error in the spread plate method:
1. A bacterial culture was grown for 12 hours. At 4-hour interval, the culture was sampled to determine the population ofthe culture, by transferring 25 mlof the suspension to 225 ml 0.90% NaCl. Three consecutive dilutions were further made by using 1 mlaliquot in 9 mlof 0.90% NaCl. One ml from each dilution was plated in each of duplicate plates. The following table shows the results of the plating method. EDI Sampling COUNTS 1st dilution 54; 61 TNTC 2nd dilution 3; 7 TNCT TNTC 3rd dilution 0; 0 242; 233 TNTC 4th dilution 0, 0 28: 37 249;246 1st 2nd 3rd TNTC * TNTC = Too numerous to count i. Determine the bacterial count (CFU/ml) every 4 hours of incubation for 12 hours. Show all computations. a. 1s* Sampling b. 2d Sampling c. 3* Sampling

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