hen we try to study the bacterial flora of the body, soil, water, or just about any environment, e realize that bacteria exist in natural environments as mixed populations. It is only in very re instances that they occur as a single species. are cultures are required to study the cultural, morphological, and physiological aracteristics of an individual organism. Several methods for obtaining pure cultures are ailable to the microbiologist today. Two commonly used methods are streak plate methods and pour plate methods. oth types of procedures involve repeatedly diluting the cells in a sample to an end point nere a single cell divides giving rise to single pure colony. The colony is therefore assumed be the identical progeny of the original cell and can be picked and used for further study of e bacterium. eaking e drop of bacterial solution contains millions of cells. To identify a species, individual strain ve to be grown separately, starting from a single cell. The most commonly. used method t ate strain originating from a single cell is called the streaking method. There are severa erent ways to streak a plate.
hen we try to study the bacterial flora of the body, soil, water, or just about any environment, e realize that bacteria exist in natural environments as mixed populations. It is only in very re instances that they occur as a single species. are cultures are required to study the cultural, morphological, and physiological aracteristics of an individual organism. Several methods for obtaining pure cultures are ailable to the microbiologist today. Two commonly used methods are streak plate methods and pour plate methods. oth types of procedures involve repeatedly diluting the cells in a sample to an end point nere a single cell divides giving rise to single pure colony. The colony is therefore assumed be the identical progeny of the original cell and can be picked and used for further study of e bacterium. eaking e drop of bacterial solution contains millions of cells. To identify a species, individual strain ve to be grown separately, starting from a single cell. The most commonly. used method t ate strain originating from a single cell is called the streaking method. There are severa erent ways to streak a plate.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Transcribed Image Text:Pure Culture Techniques
When we try to study the bacterial flora of the body, soil, water, or just about any environment,
we realize that bacteria exist in natural environments as mixed populations. It is only in very
rare instances that they occur as a single species.
Pure cultures are required to study the cultural, morphological, and physiological
characteristics of an individual organism. Several methods for obtaining pure cultures are
available to the microbiologist today. Two commonly used methods are
• streak plate methods and
• pour plate methods.
Both types of procedures involve repeatedly diluting the cells in a sample to an end point
where a single cell divides giving rise to single pure colony. The colony is therefore assumed
to be the identical progeny of the original cell and can be picked and used for further study of
the bacterium.
Streaking
One drop of bacterial solution contains millions of cells. To identify a species, individual strains
have to be grown separately, starting from a single cell. The most commonly, used method to
isolate strain originating from a single cell is called the streaking method. There are several
different ways to streak a plate.
Figure 1: Streaking pattern on the agar plate. The colored lines indicate the path of the wire loop.
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