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1. You are interested in the eukaryotic protein/enzyme Thiolase, which is 200 amino acids in length. Because Thiolase is only active in the peroxisome (an organelle) you know there must be a peroxisomal localization signal sequence in the protein. Sub-cellular Location of GFP protein Construct GFP cytosol Thiolase GFP Peroxisome In order to be able to visualize the Thiolase protein in a cell, you create a fusion construct with the gene for Thiolase and the gene for Green Fluorescent Protein (GFP). You also make a series of deletions in the gene for Thiolase, all fused with the GFP gene. Numbers on top of the constructs represent amino acid positions. Peroxisome GFP 100 GFP Peroxisome cytosol GFP cytosol You express each construct in a mammalian cell and use a microscope to visualize where the Thiolase-GFP protein gets trafficked. GFP GFP Peroxisome Peroxisome GFP A. Using the data provided, where must the peroxisomal localization signal sequence be in the Thiolase protein? Report the amino acid number range in your answer. cytosol GFP 1 50 GFP cytosol 1 25 GFP cytosol B. The answer to above question A reveals amino acids that maybe necessary for trafficking to the proteosome. Propose an experiment which will indicate if these amino acids are sufficient for trafficking to the proteosome. 50 1 125 200 200 200 200 150 200 150 125 100

1. You are interested in the eukaryotic protein/enzyme Thiolase, which is 200 amino acids in length. Because Thiolase is only active in the peroxisome (an organelle) you know there must be a peroxisomal localization signal sequence in the protein. Sub-cellular Location of GFP protein Construct GFP cytosol Thiolase GFP Peroxisome In order to be able to visualize the Thiolase protein in a cell, you create a fusion construct with the gene for Thiolase and the gene for Green Fluorescent Protein (GFP). You also make a series of deletions in the gene for Thiolase, all fused with the GFP gene. Numbers on top of the constructs represent amino acid positions. Peroxisome GFP 100 GFP Peroxisome cytosol GFP cytosol You express each construct in a mammalian cell and use a microscope to visualize where the Thiolase-GFP protein gets trafficked. GFP GFP Peroxisome Peroxisome GFP A. Using the data provided, where must the peroxisomal localization signal sequence be in the Thiolase protein? Report the amino acid number range in your answer. cytosol GFP 1 50 GFP cytosol 1 25 GFP cytosol B. The answer to above question A reveals amino acids that maybe necessary for trafficking to the proteosome. Propose an experiment which will indicate if these amino acids are sufficient for trafficking to the proteosome. 50 1 125 200 200 200 200 150 200 150 125 100

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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1. You are interested in the eukaryotic protein/enzyme Thiolase, which is 200 amino acids in length. Because Thiolase is only active in the peroxisome (an organelle) you know there must be a peroxisomal localization signal sequence in the protein. Sub-cellular Location Construct of GFP protein In order to be able to visualize the Thiolase protein in a cell, you create a fusion Cytosol construct with the gene for Thiolase and the GFP gene for Green Fluorescent Protein (GFP). 200 Thiolase Peroxisome GFP You also make a series of deletions in the 50 200 Peroxisome gene for Thiolase, all fused with the GFP GFP 100 200 gene. Numbers on top of the constructs GFP Peroxisome represent amino acid positions. 125 200 Cytosol GFP You express each construct in a mammalian 150 200 cell and use a microscope to visualize where the Thiolase-GFP protein gets trafficked. cytosol GEP 150 Peroxisome GFP Using the data provided, where must the peroxisomal localization signal sequence be in the Thiolase protein? Report the amino acid number range in your answer. А. 125 Peroxisome GFP 100 Cytosol GFP 1 50 cytosol GFP 1 25 cytosol GFP B. The answer to above question A reveals amino acids that maybe necessary for trafficking to the proteosome. Propose an experiment which will indicate if these amino acids are sufficient for trafficking to the proteosome.
Transcribed Image Text:1. You are interested in the eukaryotic protein/enzyme Thiolase, which is 200 amino acids in length. Because Thiolase is only active in the peroxisome (an organelle) you know there must be a peroxisomal localization signal sequence in the protein. Sub-cellular Location Construct of GFP protein In order to be able to visualize the Thiolase protein in a cell, you create a fusion Cytosol construct with the gene for Thiolase and the GFP gene for Green Fluorescent Protein (GFP). 200 Thiolase Peroxisome GFP You also make a series of deletions in the 50 200 Peroxisome gene for Thiolase, all fused with the GFP GFP 100 200 gene. Numbers on top of the constructs GFP Peroxisome represent amino acid positions. 125 200 Cytosol GFP You express each construct in a mammalian 150 200 cell and use a microscope to visualize where the Thiolase-GFP protein gets trafficked. cytosol GEP 150 Peroxisome GFP Using the data provided, where must the peroxisomal localization signal sequence be in the Thiolase protein? Report the amino acid number range in your answer. А. 125 Peroxisome GFP 100 Cytosol GFP 1 50 cytosol GFP 1 25 cytosol GFP B. The answer to above question A reveals amino acids that maybe necessary for trafficking to the proteosome. Propose an experiment which will indicate if these amino acids are sufficient for trafficking to the proteosome.
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