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1. What trend did you observe for the rate of peroxidase compared to the variable tested?

1. What trend did you observe for the rate of peroxidase compared to the variable tested?

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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mpioto JCitCICC3) 1. What trend did you observe for the rate of peroxidase compared to the variable tested? 2. What was the optimum condition tested for the enzyme peroxidase? 3. Did any of the conditions tested cause the enzyme to denature? Which one(s)?
Transcribed Image Text:mpioto JCitCICC3) 1. What trend did you observe for the rate of peroxidase compared to the variable tested? 2. What was the optimum condition tested for the enzyme peroxidase? 3. Did any of the conditions tested cause the enzyme to denature? Which one(s)?
K 1 1 H202 (mL) Rate (abs/secs at 500 nm) Absorbance tubes 2 &3 Absorbance tubes 4 &:Absorbance tubes 6 & Absorbance 8 & 9 Time 2. 2.5 0.001907143 0.065 0.044 0.007 0.059 0.041 3 1.75 0.001721429 20 0.017 0.027 0.008 4 1.25 0.001728571 40 0.074 0.063 0.044 0.037 5 0.75 0.001548214 60 0.117 0.103 0.085 0.079 6. 80 0.163 0.148 0.13 0.117 7. 100 0.211 0.195 0.178 0.16 8 120 Figure 1: 10 0.0025 11 12 13 etoxidaze rate 14 0.002 15 16 17 0.0015 18 19 20 21 0.001 22 23 24 25 0.0005 26 27 28 29 30 0.5 1.5 2.5 31 H202 Concentration (ml) 32 Sheet1 (wu 0os ze sas/sqe) aze
Transcribed Image Text:K 1 1 H202 (mL) Rate (abs/secs at 500 nm) Absorbance tubes 2 &3 Absorbance tubes 4 &:Absorbance tubes 6 & Absorbance 8 & 9 Time 2. 2.5 0.001907143 0.065 0.044 0.007 0.059 0.041 3 1.75 0.001721429 20 0.017 0.027 0.008 4 1.25 0.001728571 40 0.074 0.063 0.044 0.037 5 0.75 0.001548214 60 0.117 0.103 0.085 0.079 6. 80 0.163 0.148 0.13 0.117 7. 100 0.211 0.195 0.178 0.16 8 120 Figure 1: 10 0.0025 11 12 13 etoxidaze rate 14 0.002 15 16 17 0.0015 18 19 20 21 0.001 22 23 24 25 0.0005 26 27 28 29 30 0.5 1.5 2.5 31 H202 Concentration (ml) 32 Sheet1 (wu 0os ze sas/sqe) aze
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