mpioto JCitCICC3)1. What trend did you observe for the rate of peroxidase compared to the variable tested?2. What was the optimum condition tested for the enzyme peroxidase?3. Did any of the conditions tested cause the enzyme to denature? Which one(s)? K11 H202 (mL) Rate (abs/secs at 500 nm)Absorbance tubes 2 &3 Absorbance tubes 4 &:Absorbance tubes 6 & Absorbance 8 & 9Time2.2.50.0019071430.0650.0440.0070.0590.04131.750.001721429200.0170.0270.00841.250.001728571400.0740.0630.0440.03750.750.001548214600.1170.1030.0850.0796.800.1630.1480.130.1177.1000.2110.1950.1780.168120Figure 1:100.0025111213etoxidaze rate140.0021516170.0015181920210.001222324250.000526272829300.51.52.531H202 Concentration (ml)32Sheet1(wu 0os ze sas/sqe) aze

The enzyme peroxidase is responsible for catalyzing the reaction which converts H2O2 to H2O. Here…

Answered: 1. What trend did you observe for the…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

See similar textbooks

Similar questions

Can someone help me answer non-graded bio questions pertaining to lactase I'm trying to understand it more?
From the chart below, comment on the effect of substrate concentration on the rate of reaction.
3. The cell growth in problem 1 is now transitioned to a 12.0 L continuously-stirred tank reactor (a chemostat), but the feed only contains glucose as the carbon source, and the glucose is still the limiting nutrient. The initial cell concentration is 56 g cells/L The glucose is now supplied in a feed stream at 93 g/L, entering the tank at 2.5 L/h (also called the dilution rate, D). The reactor is operated at steady state in terms of flow rate, so the exit stream leaves as 2.5 L/h as well. The specific growth rate in the continuous rector follows the Monod equation, with μmax = 0.51 h¹ and a saturation constant (Ks) of 71.4 g/L. Cell yield on glucose is 0.46 g cells/g glucose. A. Use the Monod equation to determine the specific growth rate for the cells. (assume the reactor has an initial glucose concentration of 93 g/L, and that the cells are in the exponential growth phase) B. Using the initial concentration of cells and the growth rate found in part A, what is the rate of new cell…
Can I get help on finding the appropriate amount?
3. How would you use a 3-step serial dilution to produce 1ml of a luM Glucose solution from a 1M Glucose stock? What would be the total dilution factor?
1. Determine the inactivation rate constant (K) for a microorganism for the following treated effluent sample. The effluent temperature was 20°C. If the activation energy for the disinfection reaction is 60 kJ/mole, determine the inactivation rate constant (K) at 12°C. Assume Chick's law applies. In (N/N.) Time, min 1.5 3.9 3 2.5 5.6 7.7 9.2
Explain briefly why choosing a specific standard is less important for determining the concentration of protein in the cell lysate compared to the elution fraction?
Q 01 (1 pt.): A variation of centrifugation where the solution in the centrifuge tube varies in density (less dense at the top, more dense at the bottom) is called? differential centrifugation B. isopycnic centrifugation C. fractional centrifugation D. А. variable precipitant centrifugation E. density centrifugation Q 02 (1 pt.): Which of these can either stabilize or disrupt the native fold/structure of a protein? solution pH B. temperature С. А. presence of proteases D. concentration/amount of detergent Е. solution ionic strength Q 03 (5 pt.): Draw: Draw a single/in-solution L- histidine (@ solution pH = 7.5), using a Fischer projection. Make sure to specify all charge states and give the overall charge. (Free gift: pKa1 3D 1.8, pКa2 %3D9.2, pКа3 3D 6.0) ||| || |||||
16. What evidence do you have that diffusion occurs more rapidly under warmer conditions?
1. Which of the following organisms would you use to change alkaline conditions to acidic conditions? a. B. cereus-VP + b. S. aureus- Glucose metabolism test + c. E. coli- Decarboxylase + d. P. vulgaris- Simmon's citrate + e. E. aerogenes- Gelatinase + 2. Which of the following results is similar to your carbohydrate metabolism tests results when bacterial metabolic byproducts initiated a color change of red to yellow in the agar? a. B. cereus-MR + b. S. aureus-VP+ c. E. coli- Decarboxylase + d. P. vulgaris- Simmon's citrate + e. E. aerogenes- Gelatinase + 3. After an acid-fast stain, a gram positive organism would be a. blue b. green c. cranberry red d. yellow e. orange 4. Your patient is on a diet where he consumes a lot of protein. Due to this diet and the over consumption of proteins, he is dangerously close to his blood becoming acidic. So therefore, which of the following did you administer (hypothetically) to buffer the acids? a. B. cereus: VP b. S. aureus: Decarboxylase + c. L.…
4. TLC is still useful in the lab but is being replaced by automated chromatographic methods. For example, TLC, FPLC, and HPLC are now automated processes, and computers can control various parameters, such as flow rate, blends of solvent mixtures, etc. Can you think of how the ability to manipulate the parameters of these automated chromatographic methods (sample size, column plate length, flow rate, solvent gradients, etc.) might accentuate or improve an investigator's ability to separate amino acids or other biomolecules?
i. What are the advantages of biological value of protein method compared to N-balance and chemical score? ii. A bull consumes 7 kg DM of feed containing 60 g N/kg. The bull excretes 120 g N in the faeces and 50 g N in the urine. The feacal and urinary N had 20 g metabolic feacal nitrogen (MFN) and 6 g endogenous urinary nitrogen (EUN) respectively. Calculate the BV of the protein in the diet.

SEE MORE QUESTIONS

Recommended textbooks for you

Biochemistry

ISBN:

9781319114671

Author:

Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:

W. H. Freeman

Lehninger Principles of Biochemistry

Biochemistry

ISBN:

9781464126116

Author:

David L. Nelson, Michael M. Cox

Publisher:

W. H. Freeman

Fundamentals of Biochemistry: Life at the Molecul…

Biochemistry

ISBN:

9781118918401

Author:

Donald Voet, Judith G. Voet, Charlotte W. Pratt

Publisher:

WILEY

Biochemistry

ISBN:

9781319114671

Author:

Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:

W. H. Freeman

Lehninger Principles of Biochemistry

Biochemistry

ISBN:

9781464126116

Author:

David L. Nelson, Michael M. Cox

Publisher:

W. H. Freeman

Fundamentals of Biochemistry: Life at the Molecul…

Biochemistry

ISBN:

9781118918401

Author:

Donald Voet, Judith G. Voet, Charlotte W. Pratt

Publisher:

WILEY

Biochemistry

ISBN:

9781305961135

Author:

Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal

Publisher:

Cengage Learning

Biochemistry

ISBN:

9781305577206

Author:

Reginald H. Garrett, Charles M. Grisham

Publisher:

Cengage Learning

Fundamentals of General, Organic, and Biological …

Biochemistry

ISBN:

9780134015187

Author:

John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson

Publisher:

PEARSON

SEE MORE TEXTBOOKS