2. Suppose you had allowed your dialysis experiment to continue for several days(with the beaker covered to prevent evaporation). At the end of that time period,you cut open the dialysis bag and pour the contents of the sausage into a cleanbeaker. If you were to test that sausage solution, what would you expect to bepresent in it?Why?OSU

As given in the question, the dialysis experiment was continued for days with the beaker covered,…

Answered: 2. Suppose you had allowed your…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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3. Equal numbers of fibroblasts and endothelial cells are present initially in a culture. a. If the endothelial cells double every 40 hours and the fibroblasts double every 20 hours, draw a graph showing the percentage of cells in the culture that are fibroblasts as a function of time (you can use excel). b. Come up with an equation to determine the time at which 90% of the cells will be fibroblasts and solve for time. Explain how you came up with your equation.
7. Which of the following can be achieved using dialysis? I. Desalting II. Concentration III. Buffer exchange IV. Cell harvesting A. I and III B. All except II C. All except IV D. All of the above
1.Determine one kind of biological molecule that Benedict's regent, Biuret reagent, or iodine-potassium-iodide (l2-KI) solution cannot detect. 2. What variable influences the rate at which solutes and water pass through the dialysis tubing?
4. TLC is still useful in the lab but is being replaced by automated chromatographic methods. For example, TLC, FPLC, and HPLC are now automated processes, and computers can control various parameters, such as flow rate, blends of solvent mixtures, etc. Can you think of how the ability to manipulate the parameters of these automated chromatographic methods (sample size, column plate length, flow rate, solvent gradients, etc.) might accentuate or improve an investigator's ability to separate amino acids or other biomolecules?
A donor underwent physical examination and was assessed as healthy with no known illnesses. The donor’s kidney was subsequently transplanted to a recipient. Transplantation was successful. Not long after the transplantation, the recipient was diagnosed with kidney cancer. Can you make an educated speculation as to why the recipient, who had no history of kidney cancer or any other types of cancer, developed kidney after the transplantation?
3. Explain the reason for the following rules:a. Always set the micropipette within its designated range f. Always watch to see the solution enter the tipg. After expelling liquid, always hold the plunger at the second stop while removing the pipette from the liquidh. Always balance a centrifuge before turning it on
35. Actually the interstitial fluid is formed through the process of: a. Capillary reabsorption b. Capillary filtration c. Cellular metabolism d. Genetic mutation
5. A patient receives a donor kidney to replace diseased kidney tissue. Consider and answer the following: A) Describe the mechanism that was used to identify a matching donor.
1. An erythrocyte placed into a hypertonic solution will display which of the following effects? flaccidity plasmolysis crenation hemolysis turgidity
What would happen if you had a hole in the membrane of the dialysis cassette and put it in the dialysis buffer?
4. What is the primary purpose of haemodialysis? Oa. To remove excess fluids from the body Ob. To remove waste products from the blood Oc. To increase the production of red blood cells Od. To decrease the production of white blood cells

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