1. What is the chemical basis for a positive test in Molisch test? 2. What is the chemical basis for a positive test in Benedict's test? 3. What is the chemical basis for a positive test in Iodine's test?
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- 1. Why is it necessary to avoid underfilled blood samples in anticoagulant evacuated tubes? 2. Explain the purpose of thixotropic gel in a blood collection tube. 3. What is the rationale behind the sequence of the order of draw? a. Sterile tube (blood culture) b. Blue-top coagulation tube C. Serum tube (glass) with no additive d. Serum tube with clot activator, with or without е. Heparin tube with or without gel plasma separator f. EDTA tube g. Glycolytic inhibitor tube 4. What are the potentially affected tests if an evacuate tube with silica clot activator was filled first before a blue-top coagulation tube?REACTIONS OF CARBOHYDRATES Reagents:Samples:10% glucose, galactose, fructose, xylose, maltose, sucrose, lactose, starch (5 ml each) ,2 ml Fehling's A, 2 ml Fehling's B, 8 ml Benedict's reagent, 8 ml Barfoed's reagent, 3 g sodium acetate, 2 g phenylhydrazineHCl Materials: 12( 20 ml) test tubes, test tube rack, test tube brush, test tube holder, alcohol lamp, tripod, wire gauze, water bath, (10 ml) graduated cylinder, dropper, stirring rod, beaker(250 ml), spatula, microscope,7 glass slide, 7 cover slip, watch glass, platform balance Procedure: 1. Osazone formation –Mix 3 g sodium acetate and 2 g phenylhydrazineHCL and 14 ml distilled water. Warm with stirring until solution clears. Place 1 ml sugar solution (glucose, fructose, galactose, maltose, xylose, sucrose, lactose) in separate tube. Add 2 ml of the hot solution in each of the sugar solution, stopper with cotton, mix well and heat in a boiling water bath for 30 minutes. Cool at room temperature and examine the crystals under the…1. Compare and contrast DAT from IAT based on procedure and clinical significance. 2. Explain the importance of the three phases of Indirect Coomb's test. 3. Differentiate the three potentiators used in Coomb's Test (PEG, LISS, 22% BSA)
- What is Hemin test and Benzidine test?Why is there a need to puncture the skin at 3mm depth in doing the Duke's bleeding time test?1. cite the differences between tube dilution and microtiter plate dilution. 2. why is it important to isolate the peripheral blood mononuclear cells completely from the other cellular components of the blood? 3. what laboratory assays can be performed to assess the efficiency of the sample preparation and isolation of the PBMC?
- 1. Conclude regarding the chemical composition of the formation of film in fresh milk in biuret test and acrolein test.111.What factors are involved in determining the significance of an isolate from a positive blood culture? 2.Is it important to have a full identification on every positive blood culture isolate?
- Draw the steps of Qualitative Slide Test in LATEX AGGLUTINATION TEST FOR THE DETECTION OF RHEUMATOID FACTOR: SCREEN AND TITER 1. Bring all reagents and test samples to room temperature before use. 2. Make a 1:20 dilution of the patient serum by pipetting 0.1 ml of patient serum into 1.9 mL of 1X diluent or saline in a test tube. Pipet 50 µl of the diluted sample onto one test circle on the slide. 3. Place one drop (about 50 mL) of positive control onto a separate test circle and one drop of negative control onto another test circle. 4. Invert the latex reagent bottle several times to mix the beads into the suspension. Add one drop of well-shaken reagent to each test area of the slide. 5. Mix each test area with a disposable stirrer, spreading each mixture over the entire section. Use a clean disposable stirrer for each test area. 6. Rock the slide gently with a rotary motion for 2 minutes and observe immediately for agglutination.What are the chemical reaction of the following Blood Additives/Anticoagulants? 1. None 2. Clot activator 3. Clot activator separation gel 4. K3 EDTA (liquid form) 5. K2 EDTA (spray-dried) 6. 3.2% sodium citrate 7. 3.8% sodium citrate 8. Sodium heparin, lithium heparin 9. Sodium flouride 10. Sodium polyanetholesulfonate (SPS)Draw the steps of Qualitative Test in LATEX AGGLUTINATION TEST FOR THE DETECTION OF RHEUMATOID FACTOR 1. Bring the test reagents and samples to room temperature (Note 1). 2. Shake the latex vial gently. Aspirate dropper several times to obtain a thorough mixing. 3. Place 1 drop (50 µL) of the serum under test into one of the circles on the card. Dispense 1 drop of positive control serum and 1 drop of negative control into two additional circles. 4. Add 1 drop of ASLO-Latex Antigen to each circle next to the sample to be tested. 5. Mix the contents of each circle with a disposable stirrer while spreading over the entire area enclosed by the ring. Use separate stirrers for each mixture. 6. Rotate the slide by means of a mechanical rotator (100 r.p.m.) for a period of 2 minutes (Note 2). 7. Observe immediately under a suitable light source for any degree of agglutination.