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Hyrolysis of DNA Test Results 1. Inorganic Phosphate 2. Purines 3. Deoxyribose

Hyrolysis of DNA Test Results 1. Inorganic Phosphate 2. Purines 3. Deoxyribose

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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1. Explain the formation of precipitate in the test for purines.

2. Fill in the table

Procedure: Preparation of precipitating solution: 1. Place 1 spoonful of NaCl and 5 ml of liquid soap in 150 ml beaker. 2. Fill half full with hot water and mix. This is the precipitating solution. 3. In a separate 250 ml beaker, place slice banana and half fill with distilled water. 4. Add 5 tablespoon of precipitating solution and blend using blender. 5. Filter in a 250 ml beaker using cheesecloth. 6. Fill the beaker cold isopropyl alcohol 7. Let it stand and collect the fibrous mass that appears. 8. This is the DNA. HYDROLYSIS OF DNA A. To the fibrous mass, add 10 ml of 10% H2SO4 in a beaker. Boil gently or 5 minutes. Perform the following test: 1. Inorganic phosphate – to 1 ml NHẠOH, add 1 ml filtrate. Acidify by placing a small piece of blue litmus paper using 10% HNO; until the litmus paper changes to red. Then add 2 ml ammonium molybdate. Boil the solution and allow to stand for a few minutes. Note color of ppt. 2. Purines - to 2 ml filtrate add 3 ml of 3M NH,OH. Add 2-3 drops of 0.1M AGNO3 solution to it. Note color of precipitate. 3. Deoxyribose – to 1 ml filtrate add 1 ml of diphenylamine reagent. Heat for 10 mins in a boiling water bath. Cool and note color produced.
Transcribed Image Text:Procedure: Preparation of precipitating solution: 1. Place 1 spoonful of NaCl and 5 ml of liquid soap in 150 ml beaker. 2. Fill half full with hot water and mix. This is the precipitating solution. 3. In a separate 250 ml beaker, place slice banana and half fill with distilled water. 4. Add 5 tablespoon of precipitating solution and blend using blender. 5. Filter in a 250 ml beaker using cheesecloth. 6. Fill the beaker cold isopropyl alcohol 7. Let it stand and collect the fibrous mass that appears. 8. This is the DNA. HYDROLYSIS OF DNA A. To the fibrous mass, add 10 ml of 10% H2SO4 in a beaker. Boil gently or 5 minutes. Perform the following test: 1. Inorganic phosphate – to 1 ml NHẠOH, add 1 ml filtrate. Acidify by placing a small piece of blue litmus paper using 10% HNO; until the litmus paper changes to red. Then add 2 ml ammonium molybdate. Boil the solution and allow to stand for a few minutes. Note color of ppt. 2. Purines - to 2 ml filtrate add 3 ml of 3M NH,OH. Add 2-3 drops of 0.1M AGNO3 solution to it. Note color of precipitate. 3. Deoxyribose – to 1 ml filtrate add 1 ml of diphenylamine reagent. Heat for 10 mins in a boiling water bath. Cool and note color produced.
Hyrolysis of DNA Test Results 1. Inorganic Phosphate 2. Purines 3. Deoxyribose
Transcribed Image Text:Hyrolysis of DNA Test Results 1. Inorganic Phosphate 2. Purines 3. Deoxyribose
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