1. If the mixture is subjected to gel filtration chromatography, which protein component will elute first? 2. If the mixture is subjected to isoelectric focusing, which protein will stop moving nearest to the positive electrode? 3. If the mixture is subjected to cation-exchange chromatography using a buffer at pH 7, which protein will bind to the resin? 4. If the mixture is subjected to SDS-PAGE, which protein will be at the bottommost
1. If the mixture is subjected to gel filtration chromatography, which protein component will elute first? 2. If the mixture is subjected to isoelectric focusing, which protein will stop moving nearest to the positive electrode? 3. If the mixture is subjected to cation-exchange chromatography using a buffer at pH 7, which protein will bind to the resin? 4. If the mixture is subjected to SDS-PAGE, which protein will be at the bottommost
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
Related questions
Question
100%
![Consider the following properties of the protein components of a sample mixture as
provided in the table below.
Protein
Molecular
IpH
Percentage of
polar amino
acid residues
Weight (kDa)
(%)
АСЕ
200
7
20
CLU
25
65
DIA
100
10
40
НЕА
50
80](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F7f0f0d0f-7a91-48f0-b947-d8de72a9c807%2Fa3b89807-f942-49d2-88cb-4b331c807c8c%2Fezagcfk_processed.jpeg&w=3840&q=75)
Transcribed Image Text:Consider the following properties of the protein components of a sample mixture as
provided in the table below.
Protein
Molecular
IpH
Percentage of
polar amino
acid residues
Weight (kDa)
(%)
АСЕ
200
7
20
CLU
25
65
DIA
100
10
40
НЕА
50
80
![1. If the mixture is subjected to gel filtration chromatography, which protein
component will elute first?
2. If the mixture is subjected to isoelectric focusing, which protein will stop moving
nearest to the positive electrode?
3. If the mixture is subjected to cation-exchange chromatography using a buffer at pH
7, which protein will bind to the resin?
4. If the mixture is subjected to SDS-PAGE, which protein will be at the bottommost
portion of the gel?
5. If the mixture is subjected to hydrophobic interaction chromatography, which
protein will bind most strongly to the resin?](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F7f0f0d0f-7a91-48f0-b947-d8de72a9c807%2Fa3b89807-f942-49d2-88cb-4b331c807c8c%2Fgeqz1q_processed.jpeg&w=3840&q=75)
Transcribed Image Text:1. If the mixture is subjected to gel filtration chromatography, which protein
component will elute first?
2. If the mixture is subjected to isoelectric focusing, which protein will stop moving
nearest to the positive electrode?
3. If the mixture is subjected to cation-exchange chromatography using a buffer at pH
7, which protein will bind to the resin?
4. If the mixture is subjected to SDS-PAGE, which protein will be at the bottommost
portion of the gel?
5. If the mixture is subjected to hydrophobic interaction chromatography, which
protein will bind most strongly to the resin?
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