Consider the following properties of the protein components of a sample mixture asprovided in the table below.ProteinMolecularIpHPercentage ofpolar aminoacid residuesWeight (kDa)(%)АСЕ200720CLU2565DIA1001040НЕА5080 1. If the mixture is subjected to gel filtration chromatography, which proteincomponent will elute first?2. If the mixture is subjected to isoelectric focusing, which protein will stop movingnearest to the positive electrode?3. If the mixture is subjected to cation-exchange chromatography using a buffer at pH7, which protein will bind to the resin?4. If the mixture is subjected to SDS-PAGE, which protein will be at the bottommostportion of the gel?5. If the mixture is subjected to hydrophobic interaction chromatography, whichprotein will bind most strongly to the resin?

In gel filtration chromatography the stationary phase is a porous matrix and the mobile phase is the…

Answered: 1. If the mixture is subjected to gel…

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter6: Basic Clinical Chemistry

Section6.6: Blood Cholesterol And Triglycerides

Problem 4CS

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a. Compounds A, B, C, and D are known to be intermediates in the pathway for production of protein E. To determine where the block in protein-E production occurred in each individual, the various intermediates were given to each individuals cel Is in culture. After a few weeks of growth with the intermediate, the cells were assayed for the production of protein E. The results for each individuals cells are given in the following table. A plus sign means that protein E was produced after the cells were given the intermediate listed at the top of the column. A minus sign means that the cells still could not produce protein E even after being exposed to the intermediate at the top of the column. Draw the pathway leading to the production of protein E.
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