(1) Enzy Matic, a Biochemistry student is studying the mode of inhibition of maleate to fumarase enzyme. Fumarase is the enzyme in the Kreb's cycle that converts fumarate to malate. He obtained the following kinetics data from her experiment: [S], mM 1.75 2.17 3.00 5.50 10.5 Velocity (mM/min) 1/[S], mM-1 Uninhibited 1.94 2.26 2.85 3.55 4.39 Inhibited 1.38 1.67 2.13 2.97 3.83 (a) Compute for the values of 1/[S] and 1/v for both the uninhibited and inhibited reactions. (10 pts) 1/Velocity (min/mm) Uninhibited Inhibited (b) Use MS Excel® program to construct the Lineweaver-Burk plots for both reaction systems. Conduct linear regression analysis and compute for the equation of the lines and their respective R² values. Compute for the Vmax and KM values of the uninhibited and inhibited reaction. Determine the mode of inhibition based on the generated graph and the values of the kinetic parameters. Print your result and attach it to this work sheet. (20 pts)
(1) Enzy Matic, a Biochemistry student is studying the mode of inhibition of maleate to fumarase enzyme. Fumarase is the enzyme in the Kreb's cycle that converts fumarate to malate. He obtained the following kinetics data from her experiment: [S], mM 1.75 2.17 3.00 5.50 10.5 Velocity (mM/min) 1/[S], mM-1 Uninhibited 1.94 2.26 2.85 3.55 4.39 Inhibited 1.38 1.67 2.13 2.97 3.83 (a) Compute for the values of 1/[S] and 1/v for both the uninhibited and inhibited reactions. (10 pts) 1/Velocity (min/mm) Uninhibited Inhibited (b) Use MS Excel® program to construct the Lineweaver-Burk plots for both reaction systems. Conduct linear regression analysis and compute for the equation of the lines and their respective R² values. Compute for the Vmax and KM values of the uninhibited and inhibited reaction. Determine the mode of inhibition based on the generated graph and the values of the kinetic parameters. Print your result and attach it to this work sheet. (20 pts)
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
Related questions
Question
Paste your Microsoft Excel Graph of the kinetic analysis. Compute for Vmax and Km values and identify the mode of enzyme inhibition involved.
![(1) Enzy Matic, a Biochemistry student is studying the mode of inhibition of maleate to fumarase enzyme.
Fumarase is the enzyme in the Kreb's cycle that converts fumarate to malate. He obtained the
following kinetics data from her experiment:
[S], mM
1.75
2.17
3.00
5.50
10.5
Velocity (mm/min)
1/[S], mM-1
Uninhibited
1.94
2.26
2.85
3.55
4.39
(a) Compute for the values of 1/[S] and 1/v for both the uninhibited and inhibited reactions. (10 pts)
1/Velocity (min/mm)
Uninhibited
Inhibited
1.38
1.67
2.13
2.97
3.83
Inhibited
(b) Use MS Excel® program to construct the Lineweaver-Burk plots for both reaction systems.
Conduct linear regression analysis and compute for the equation of the lines and their respective
R² values. Compute for the Vmax and KM values of the uninhibited and inhibited reaction. Determine
the mode of inhibition based on the generated graph and the values of the kinetic parameters.
Print your result and attach it to this work sheet. (20 pts)](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F24afbc89-6a82-4988-8a09-9fcaf4ed2f90%2Faf6b47f5-6abd-4077-b427-f49ceff12e15%2Fdhncjzm_processed.png&w=3840&q=75)
Transcribed Image Text:(1) Enzy Matic, a Biochemistry student is studying the mode of inhibition of maleate to fumarase enzyme.
Fumarase is the enzyme in the Kreb's cycle that converts fumarate to malate. He obtained the
following kinetics data from her experiment:
[S], mM
1.75
2.17
3.00
5.50
10.5
Velocity (mm/min)
1/[S], mM-1
Uninhibited
1.94
2.26
2.85
3.55
4.39
(a) Compute for the values of 1/[S] and 1/v for both the uninhibited and inhibited reactions. (10 pts)
1/Velocity (min/mm)
Uninhibited
Inhibited
1.38
1.67
2.13
2.97
3.83
Inhibited
(b) Use MS Excel® program to construct the Lineweaver-Burk plots for both reaction systems.
Conduct linear regression analysis and compute for the equation of the lines and their respective
R² values. Compute for the Vmax and KM values of the uninhibited and inhibited reaction. Determine
the mode of inhibition based on the generated graph and the values of the kinetic parameters.
Print your result and attach it to this work sheet. (20 pts)
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