........ cen not be true about in ditu hybridization. Use to reveal where gene expressed at mrna lvel.   perform in none live animal/ tissues. reveal whether 2 genes are active in same cell. determine the function of gene

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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........ cen not be true about in ditu hybridization.

Use to reveal where gene expressed at mrna lvel.

 

perform in none live animal/ tissues.

reveal whether 2 genes are active in same cell.

determine the function of gene

Expert Solution
Step 1

In-situ hybridization spots specific nucleic acid sequences within intact chromosomes, eukaryotic cells, or bacterial cells through the use of specific nucleic acid-labeled probes. It is used to reveal the location of specific nucleic acid sequences on chromosomes or in tissues, a crucial step for understanding the organization, regulation, and function of genes.

In-situ probes are:

  • Double-stranded DNA (dsDNA) probes
  • Single-stranded DNA (ssDNA) probes
  • RNA probes (riboprobes)
  • Synthetic oligonucleotides (PNA, LNA)

Application of In-situ-hybridization are:

  • Microbiology (classic target - 16S rRNA) 
  • Pathology (pathogen profiling, abnormal gene expression)
  • Developmental biology (gene expression profiling in embryonic tissues)
  • Karyotyping and phylogenetic analysis 
  • Physical mapping 
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