Lab 5 REPORT Gregory Chekerdjian

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Purdue University *

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11500

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Health Science

Date

Feb 20, 2024

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Lab 5: Which Cereal Contains the Most Iron? REPORT FORM Question #0. Insert a photo of your data sheet from lab below. Question #1. Insert a screenshot of your calibration plot (standard curve) with the best-fit line. Include a title, axis labels and R 2 value. These components can be written on the graph with a stylus or a text-box if needed. Title: How does change in concentration influence the absorbance R = 0.9994 R 2 =0.9988 A = 8933C + 0.02124
Question #2. Using the absorbance value for your cereal sample calculate the concentration of Fe 2+ in your original 100.00 mL solution. You diluted your sample solution twice: when you transferred a measured volume from the 100mL volumetric flask to another volumetric flask and again when you transferred a measured volume to a test tube and added KSCN. Use the concentration (molarity) of iron from above and the dilution factors to determine the concentration of iron in the original 100 mL volumetric flask. The absorbance value for your cereal sample is: 0.293
Question #3. Calculate the experimental mass of Fe(s) (mg) in your cereal sample using your experimental results. Use the concentration of [Fe(SCN) 6 ] 3- in the original 100-mL volumetric flask to determine the mass of iron (in milligrams) in your original cereal sample (of about 3 grams). Question #4. Calculate the experimental mass of Fe(s) (mg) in a serving size of your cereal using your experimental results. Refer to the information you recorded earlier in your laboratory notebook for the mass (in g) of a serving size of the cereal you analyzed. Using the mass of iron in your cereal sample calculated above; determine the experimental mass (in mg) of iron in a serving size .
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Question #5. Compare the experimental mass of Fe(s) in a serving size to the actual value on the cereal box. Show a calculation to support your answer. (Hint: Which formula from Lab 1 is appropriate for this comparison?) Question #6. Based on the class results, what conclusions can you draw about the iron content of these three types of cereal? Which type has the most iron according to class results? Which cereal has more iron according to the cereal labels? Refer to specific results in your explanation. (To answer this question, you will need to calculate and report the mass of iron in a serving size of the other types of cereal using the data provided by the other groups in your lab section.) Question #7. Based on your results and the class results, comment on the effectiveness of this technique for extracting iron from cereal. What do you think were possible sources of error in this experiment? Note whether the source of error would cause the result to be falsely low or falsely high. Explain. Although we each did different cereal and might have each done different mistake the overall procedure was the same so we can find out which type of cereal had the most iron. According to our class results as seen in the picture on the left the cereal with the most iron was the Total cereal. As many of us know it is almost impossible for us to have a perfect experiment however, we can work towards getting close. The techniques used for extracting iron from cereal were effective and resulted with us having a 28% error. The reason why I got a high percent error was because I might have a made a mistake in my calculations. Another issue that caused a high percent error is that we know we should always do several tries and find the average of them however we didn’t have enough class time, so we only had to take the result of one
Question #8. What is the recommended amount of iron that should be consumed daily? Based on this number and your experimental results is cereal a good source of iron for the average adult? Why or why not? Cite your sources and refer to your data. The recommended amount of iron that should be consumed daily varies based upon age and sex. However, when looking at the average intake recommended for adults from ages 19 to 50 the recommend amount was around 8 milligrams for males and 18 milligrams for females. Therefore, if we look at our data then it is evident that cereal is a good source of iron for both children and adults. To add to that the national institute of health said that “ In the United States, about half of dietary iron comes from cereal and other grain products.” So, for a fact many people use cereal as their main source of iron. Sources: National Institute of Health. “Office of Dietary Supplements - Iron.” NIH Office of Dietary Supplements , U.S. Department of Health and Human Services, 5 Apr. 2022, https://ods.od.nih.gov/factsheets/Iron-HealthProfessional/.