Lab Assignment 3 Nerve Velocity Hand Dominance (1)

docx

School

University of Missouri, Columbia *

*We aren’t endorsed by this school

Course

3700

Subject

Chemistry

Date

Jan 9, 2024

Type

docx

Pages

Uploaded by MajorJackalMaster971

Names of all group members present: Group members NOT present: Refer to the documents “Nerve Velocity – Hand Dominance Labs and Nerve Velocity – Hand Dominance background” located on Canvas as needed. OBJECTIVE : To build upon previous lab knowledge on action potentials, stimulus strength, and nerve conduction velocity. To determine the effect of handedness on nerve conduction velocity. To complete this assignment, you only need to collect data from ONE group member. In this lab we will be following similar instructions as in Lab 2: stimulating the median nerve with an electrical impulse, measuring the time to generate an action potential and its amplitude. Follow the LabScribe instructions for collecting action potential data using “Nerve Velocity Hand Dominance Labs – Exercise 1 and Exercise 2” . However, stop at Data Analysis and refer instead to the Data Analysis instructions below. Then, follow the instructions for Exercise 3 and 4 in the labs document in their entirety. Record the results of reaction times in the corresponding Data Analysis section below. Lab 3 Assignment (15 pts total) A Priori Hypothesis: State a scientific hypothesis before collecting data for each of our three comparisons. Provide a sentence or two to explain why these are your hypotheses. (1 point) 1. Nerve conduction velocity between hands: 2. Reaction time between hands: 3. Dominant hand nerve conduction velocity versus dominant hand reaction time (i.e., what differences do you expect with each comparison). DATA ANALYSIS Exercise 1: Dominant Hand 1. What is your subject’s maximum stimulus amplitude (the highest shock stimulus needed to produce the tallest CAP (action potential)? (0.5 point): _______________mV Note: This is the amplitude that you will use at ALL arm locations

After you have 3 action potentials from each location (short distance & long distance = 6 action potentials total) from the dominant hand, follow the instructions below to measure each action potential and complete the Table in question 2. Click the 2-Cursor icon in the LabScribe toolbar. Drag one cursor to the left edge of the stimulus artifact signal and the second cursor to the peak of the muscle response. The value for V2-V1 (V=Amplitude) is in the top right of the Muscle Wave channel. The value for T2-T1 (T=Conduction Time) is in the top right above the V2-V1 value. Record measurements in the table below. 2. Enter the results for your subject’s dominant hand action potentials in the table below. (1 point) Group Member Name: Short Distance from black ROAM electrode (mm):_______ Be sure to specify distance above Long Distance from black ROAM electrode (mm):_______ Be sure to specify distance above Replicate Conduction Time Amplitude Conduction Time Amplitude 1 2 3 Which hand is this individual’s dominant hand? ___________ Please show your work for the calculations below and be sure to label units: 1. Calculate the average amplitude of the three action potentials for each distance. (0.5 point) Short Distance _________ Long Distance __________ 2. Calculate the ∆D, the difference between the long (B) and short (A) distances (mm). (0.5 point) DB-DA = ∆D Result:

3. Calculate the ∆T, the difference in the conduction time between the two distances (ms) for each of the three replicates (0.5 point) T B -T A = ∆T Replicate 1 Replicate 2 Replicate 3 4. Calculate the NCV, the Nerve Conduction Velocity (mm/ms or m/s) for each of the 3 replicates. Then calculate the average NCV. (0.5 point) NCV= ∆D/∆T Replicate 1 Replicate 2 Replicate 3 Average NCV: Exercise 2: Non-dominant Hand 1. Enter the results for your subject’s non-dominant hand action potentials in the table below. (1 point) Note: This is the same amplitude used on the dominant arm Group Member Name: Short Distance from black ROAM electrode (mm): this is the same distance you used in the dominant arm Long Distance from black ROAM electrode (mm): this is the same distance you used in the dominant arm Replicate Conduction Time Amplitude Conduction Time Amplitude 1 2 3 Please show your work for the calculations below and be sure to label units:

Your preview ends here

Eager to read complete document? Join bartleby learn and gain access to the full version

Access to all documents
Unlimited textbook solutions
24/7 expert homework help

5. Calculate the average amplitude of the three action potentials for each distance. (0.5 point) Short Distance _________ Long Distance __________ 6. Calculate the ∆D, the difference between the long (B) and short (A) distances (mm). (0.5 point) DB-DA = ∆D Result: 7. Calculate the ∆T, the difference in the conduction time between the two distances (ms) for each of the three replicates (0.5 point) T B -T A = ∆T Replicate 1 Replicate 2 Replicate 3 8. Calculate the NCV, the Nerve Conduction Velocity (mm/ms or m/s) for each of the 3 replicates. Then calculate the average NCV. (0.5 point) NCV= ∆D/∆T Replicate 1 Replicate 2 Replicate 3 Average NCV:

Exercise 3 & 4: Reaction Time and Visual Signals with the Dominant and Non-Dominant Hands Record the reaction times (in msec) for each of the subject’s 10 trials below (1 point): Trial Dominant Hand Non-Dominant Hand 1 2 3 4 5 6 7 8 9 10 Calculate the average reaction time for each hand (0.5 point). Dominant Hand ____________ Non-Dominant Hand ____________ Once you’ve collected your data, add your subject’s average NCV and average reaction time for each hand to the class dataset on Canvas. You will use the class dataset to answer the questions below. Answer the following questions completely: 1. Based on reviewing the class dataset, is nerve velocity different in the dominant vs. non- dominant arm? How did you confidently determine this? Be specific about your analysis methods and your conclusion. Provide any and all calculations, statistical results, and graphs that strengthen your claim. (1.5 points) 2. Based on reviewing the class dataset, does handedness affect the reaction time to the visual stimulus? How did you confidently determine this? Be specific about your analysis methods and your conclusion. Provide any and all calculations, statistical results, and graphs that strengthen your claim. (1.5 points) 3. What could be some biological reasons for your answers to question 2 above? Cite any sources you referenced for your answer. (1 point)

4. Plot the average class data for dominant hand nerve conduction velocity as a scatter plot over dominant hand reaction time . Place a picture of your graph below. Just by looking at this plot, does nerve velocity seem to have any effect on handedness or is it the other way around? Are there any patterns (i.e. both measures increasing or decreasing together, or no relationship) that you notice? Do you notice any outliers? Explain what you think this data means. (2 points)

Your preview ends here

Eager to read complete document? Join bartleby learn and gain access to the full version