CH223_Ch_24_Intro_Spectroscopy

pptx

School

University of Alabama *

*We aren’t endorsed by this school

Course

101

Subject

Chemistry

Date

Oct 30, 2023

Type

pptx

Pages

17

Uploaded by KidSwanPerson995

Report
Chapter 24 – Introduction to Spectroscopy Spectroscopy (or Spectrometry or Spectrophotometry) – uses absorption, emission, or reflectance of light by a material to gain info on concentration (quantitative analysis) or structure (qualitative analysis). Light: Has particle and wave characteristics. Electrical component – force from charge build-up Magnetic component – force from charge movement
Describing Light
Your preview ends here
Eager to read complete document? Join bartleby learn and gain access to the full version
  • Access to all documents
  • Unlimited textbook solutions
  • 24/7 expert homework help
The Electromagnetic Spectrum We’ll focus on the ultraviolet (UV, 180-380 nm) and visible regions (380-780 nm). Which is higher in energy?
Another View of the E-M Spectrum Wavelengths in the UV-visible region are about the width of bacteria. Which is higher in energy: red light or blue light?
Quantum Theory - Absorption Electrons are in discrete energy levels (electronic, vibration, rotational). Absorption of light: M + h M* - Energy is added to the molecule, atom, or ion. - An electron goes from ground state (M) energy level to an excited state (M*) energy level. - The time involved is usually 10 -9 to 10 -8 seconds.
Your preview ends here
Eager to read complete document? Join bartleby learn and gain access to the full version
  • Access to all documents
  • Unlimited textbook solutions
  • 24/7 expert homework help
Quantum Theory - Emission Emission of light: M* M + h - Energy is given off from an excited molecule, atom, or ion. - An electron goes from an excited state (M*) energy level to a lower energy level, which is often the ground state (M). - When the analyte was excited by a chemical reaction, the process is known as chemiluminescence .
Visible Absorption Spectra of 1,2,4,5-Tetrazine Absorbed photons must have exactly the energy difference between 2 levels. (a) Gas phase: Molecules move freely. Rotational, vibrational, and electronic energy levels are all accessible. (b) Non-polar solvent (hexane): Restricted movement, plus interactions between solvent and analyte, yield many vibrational levels. Fine structure is lost. Only electronic transitions are seen. (c) Polar solvent (water): Intermolecular forces (e.g., H-bonding) result in a large number of vibrational levels, which blend to give a single very broad peak.
Colors of Materials - White light contains all visible wavelengths. - Black is the absence of visible light. - Light absorbed by a material is not seen. - Light transmitted (passed through) or reflected (bounced off) is seen and gives the color of the material.
Your preview ends here
Eager to read complete document? Join bartleby learn and gain access to the full version
  • Access to all documents
  • Unlimited textbook solutions
  • 24/7 expert homework help
Wavelength for Quantification - Use wavelength of maximum absorbance, max . - Only exception is when there’s an interference at max . - Scan of absorbance as a function of wavelength is a spectrum .
Relating Concentration to Absorption As light passes through an absorbing species, the intensity of the light is attenuated (reduced). P 0 = power (intensity or number of photons, NOT energy) of monochromatic incoming light P = power of light that passes through the sample and is not absorbed T = transmittance = fraction of light passing through the sample = P/P 0 %T = Percent transmittance = T x 100% b = cell path length in cm A = absorbance = - log T = log(P 0 /P) A and T are unitless
Beer-Lambert Law (or Beer’s Law) Relationship of concentration to measured absorbance (A) in spectroscopy experiments: A = abc a = absorptivity, relates to the ability of the analyte to absorb at the selected λ When, b = path length in cm c = concentration in M Then, a = ε = molar absorptivity in L mol -1 cm -1 (aka M -1 cm -1 ) Under these conditions, Beer’s Law is written as: If a sample doesn’t absorb at the selected λ, ε = 0 and A = 0. bc A
Your preview ends here
Eager to read complete document? Join bartleby learn and gain access to the full version
  • Access to all documents
  • Unlimited textbook solutions
  • 24/7 expert homework help
In-Class Problem 1 Trace amounts of iron in drinking water can be determined by forming an intensely colored (orange-red) 1:3 complex of Fe(II) with o-phenanthroline. A standard solution containing 5.36 x 10 -6 M Fe(II) exhibits a percent transmittance of 76% in a 2.00-cm cell at 510 nm. What is the molar absorptivity for the Fe(II)-o-phenanthroline complex at 510 nm? You’ll make this complex in CH 223 lab!
In-Class Problem 2 A 20.0 mL aliquot of drinking water is treated with hydroquinone to reduce all dissolved iron to Fe(II). o-Phenanthroline is added and the aliquot is diluted to 50.0 mL. The absorbance in a 2.00 cm cell is 0.085 at 510 nm. What is the iron content of the water in molarity? We’ll use Beer’s Law for more complicated problems and other applications in Chapter 26.
Beer’s Law is a Limiting Law A law that is true under limited conditions. Beer’s law only works at low concentrations. As concentration increases, absorbing molecules/ions are close together and may interact, changing molar absorptivity ( ε) or shifting equilibria. Also, as ε increases, Beer’s law breaks down sooner. A calibration curve: Absorbance on the y-axis. Concentration on the x-axis. Slope is bε, intercept is near zero. Slope can be used to obtain ε.
Your preview ends here
Eager to read complete document? Join bartleby learn and gain access to the full version
  • Access to all documents
  • Unlimited textbook solutions
  • 24/7 expert homework help
Light Losses Affecting Beer’s Law
Other Factors Causing Deviations - Stray light - Mismatched cells (cuvettes) - λ selection not narrow enough (polychromatic radiation) - Particles in the solution being analyzed - Fingerprints or dirt on the cuvettes pH control is often important in spectroscopy: Ex: If an analyte has HA and A forms, the forms will have different ε and λ max . The solutions must be buffered so that the same form is present in all solutions. Rule of Thumb: UV-visible spectroscopy works best when Absorbance is in the range of 0.05 - 1.5.

Browse Popular Homework Q&A

Q: Which compound has the lowest melting point? O NaCl O CaO O KBr O BaO
Q: What do you mean by Nyquist rate?
Q: M[Q6] What are the two main advantages of pulse modulation over continuous wave modulation? M[Q7]…
Q: Image of size 1024 x 800 needs to resize to one that has 640 pixels width with the same aspect…
Q: Find the Critical Values of the Standard Normal Distribution needed for a 98% confidence interval…
Q: In humans, Tongue roller and widows peak are dominant over non-tongue roller and straight hairline.…
Q: Group 1 (Ultima) 25 20 30 22 32 45 15 16 29 25 Group 2 (Placebo) 60 45 54 68 70 46 55 57 48 49…
Q: 50 tickets numbered A bag contains 1,2,3,4......50 of which five are drawn at random and arranged in…
Q: Sixty-five percent of men consider themselves knowledgeable soccer fans. If 10 men are randomly…
Q: Negative aspects of the Character Count Method and illustrations of other framing techniques And…
Q: ion of n (n ranging between 1 and 80) for (a) x = 15 and (b) x = −15. [You can plot the two cases…
Q: ne that has 640 pixels wid ame aspect ratio, what
Q: Explain Hidden Form Fields. List their advantages and disadvantages.
Q: What is Real Time Systems? Explain With Examples.
Q: M[Q6] What are the two main advantages of pulse modulation over continuous wave modulation? M[Q7]…
Q: 8. What does the number 0.05 represent in the following: t(58) = 2.001, p < 0.05? 9. How many groups…
Q: 6. Suppose scores on exams in statistics are normally distributed with an unknown population mean…
Q: Which CSS properties must be defined to create an animation? Choose two    animation-duration…
Q: P4-2) Create an orthographic projection of the following object. Draw the three standard views. 1.75…
Q: What quantity in moles of nitrogen gas at 130.0 °C and 13.3 atm would occupy a vessel of 23.4 L?
Q: M[Q6] What are the two main advantages of pulse modulation over continuous wave modulation? M[Q7]…
Q: List the applications of minimum spanning tree?