102 Ex1 Chem (1)

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University of Victoria *

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102

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Chemistry

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Feb 20, 2024

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1 Report for Chem 102 Laboratory Exercise #1 Chromatographic separation and identification of compounds in analgesics 1 Using Microsoft Word, students are to insert responses in all yellow highlighted areas. It is recommended that the report be completed without changing font size, column width, row width, margins and highlights. No photographs or handwritten work is accepted, unless specifically indicated in the lab manual. The completed report must be uploaded to the 102 Brightspace site as a .pdf file by the due date posted on Brightspace. All answers must be the student’s own work without assistance from others. Only reports which are completed using the template will be marked. Name: _Anna Hale_ Lab Section: _B03_ Quad: _2_ Date: _17/01/24_ Abstract The identity of the components of an analgesic capsule was determined to be _Acetylsalicylic Acid_ by using the technique of _thin layer chromatography (TLC)_. Data/Results Table 1. Experimental data and calculated R f values for _Acetylsalicylic Acid_ (analgesic), and contents of unknown capsule -- . The observed data inserted in this table must be consistent with the observed data written in your In-lab notes with the correct units. Known analgesic _Acetylsalicylic Acid_ unknown analgesic _Unknown #20185_(label) Distance travel by solvent front in line with spot 1 6 cm 6 cm Distance travel by solvent front in line with spot 2 (if necessary) Distance travel by solvent front in line with spot 3 (if necessary) Distance travel by spot 1 3.3 cm 3.3 cm Distance travel by spot 2 (if necessary) Distance travel by spot 3 (if necessary) Calculated R f value for spot 1 0.55 0.55 Calculated R f value for spot 2 (if necessary) Calculated R f value for spot 3 (if necessary) Table 2. Experimental data and calculated R f values for the other two known analgesics. -- The observed data inserted in this table must be consistent with the observed data written in your laboratory notebook with the correct units. Known analgesic __Caffein__ Known analgesic __Phenacetin___ Distance travel by solvent front in line with spot 1 5 cm 5.3 cm
2 Distance travel by solvent front in line with spot 2 (if necessary) Distance travel by solvent front in line with spot 3 (if necessary) Distance travel by spot 1 1.9 cm 2.1 cm Distance travel by spot 2 (if necessary) Distance travel by spot 3 (if necessary) Calculated R f value for spot 1 0.38 0.40 Calculated R f value for spot 2 (if necessary) Calculated R f value for spot 3 (if necessary) Discussion Respond to the following: Explain how the contents of the analgesic capsule were determined (max 4 lines). _Utilizing TLC, both identified and unidentified compounds were observed on a TLC plate and introduced into a solvent. Subsequently, the plate was inspected either under a UV light or through the application of a detector solution. Rf values for the identified samples were computed using both individual data and information from colleagues. These values were then juxtaposed with the Rf value of the unknown, aiding in the determination of the present contents._ Conclusions The contents of an unknown analgesic capsule, _unkown #20185 _ were determined to be _Acetylsalicylic Acid_ by TLC. References 1. M. Reimer et al., Chemical Reactivity Fundamentals with Environmental Applications Laboratory Manual, Chemistry 102 , pp. 13-17. (University of Victoria: Victoria, B.C.). Spring 2024 . 2. _Unknown #20185. Dragon Pharmaceuticals, 9012 Jungle Ave Toronto, ON, T8T 101 Canada. LOT #AC675_ Marks max Pre-lab quiz 4 Laboratory Notebook: Have ALL data, procedures and observations been accurately recorded? 1 Report: Are ALL sections accurate and complete? Are the References formatted and cited correctly and complete? 1 Conclusions: Are the components of the unknown analgesic correctly identified? 2 Participation: 1 Student Performance: Did the student follow the safe practices guidelines without exception? 1 Total mark 10
3 © 2024, Department of Chemistry, Faculty of Science
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