Experiment 7 (1)

q41 IF YOU DO NOT KNOW THE ANSWER PLEASE DO NOT REJECT IT please calculate the unknown concentration of the protein D wih an absorbance value of A412 given the standard curve indicated in the table. write your answers in numbers only with 2 decimals. protein concentration (ug/ml) absorbance 0 0.000 0.02 0.161 0.04 0.284 0.06 0.438 0.08 0.572 0.10 0.762

Calculate the number of replicate analyses

PART ONE: Preparation of FECNS* Solution Prepare the two solutions in table 1 by accurately measuring the required volumes of distilled water and CNS into two labeled test tubes. The absorbance of the solutions must be measured soon after adding the Fe" solution from a buret. TABLE 1 Fe 5 ml 5S mL Solution Distilled Water CNS 2 mL Total Volume 3 ml 2 ml 10 ml 3 mL 10 ml In this part of the experiment you are to explore what the above quote means. As a PRELIMINARY Exercise, formulate a hypothesis as to what would happen under each of the following circumstances: 1. 1ml of 0.0020 M CNS' solution is added to the unused portion of solution 1 in the test tube 2. One drop of 0.20 M CNS solution is added to the unused portion of solution 2 in the test tube.

Use the simulation lab to obtain the distance for the solvent front and the distance for each spot and then calculate the Rf 35ig figs acetaminoph Unknown Unknown Sample caffeine ibuprofen aspirin en Mixture #1 Mixture #2 13.0 cm13.0 cm 13. O cm 13.0 cm 13.0cm Distance to 130 cm solvent front 1.77cm 1.89cm 11.1 12.6 cm 1.78cm la.6 cm 11.0 cm 6.27cm Distance Spot(s) traveled 5.58cm Calculated R:(s) Components of Unknowns D. Additional Exercises 1. Which of the substances tested is most polar? What parts of the structure of this substance is polar? 2. Which of the substance tested is most non-polar? What parts of the structure of this substance in non-polar?

If an analytical method relies on a measurable property of an analyte that can be correlated with the amount of the said analyte, what type of analytical method is it?   Oxidative methods   Instrumental methods   Blank Correction methods   Classical methods

Spot in R lane Spot in Y lane Upper spot in U lane Distance traveled by spot (cm) Lower spot in U lane 3.00 Spot in B 5.00 lane 1.20 4.70 2.90 Solvent Rf front (cm) 5.90 5.90 5.90 5.90 5.90 Based on your TLC, which best describes the identity of the unknown food dye?

Data Collection Kinetic Data Collection Time, t (s) Volume of added base (mL) First addition 40 0.50 Second addition 101 1.00 Third addition 160 1.52 Fourth addition 224 1.99 Fifth addition 278 2.49 Sixth addition 334 3.01 Seventh additlon 389 3.48 Calculations Concentration of NaOH stock solution: 0.01 M Concentration of t-BuCl stock solution: 0.2 M Volume of t-BuCl solution: 10 mL stock stock solution added to 25 mL acetone Table view List view Kinetic Data Volume of Moles of Amount of t-Bucl (mol) [-Buc] In[-BuC Time, t (s) added base added base (mL) (mol) First addition 40 0.50 Second additlon 101 1.00 Third addition 160 1.52 Fourth addition 224 1.99 Flfth additlon 278 2.49 Sixth additlon 334 3.01 Seventh addition 389 3.48 Use the data in the table above to plot In[t-BuC]]; vs time (s). Include labelled axis, a title, and a linear trendline. Upload your plot here.

please help me find concentration   Concentration of TZ stock solution (μMμM) 42.06

Gas chromatography requires a sample size. O Much, much less than a gram A gram or less 1 to 10 grams 10 to 100 grams Gas chromatography does not have a restriction on its sample size QUESTION 11 Which of the following would not have a characteristic functional group absorption in an IR spectrum? butanal benzoic acid O dimethyl ether O propanone O 1-pentanol QUESTION 12 All of the following except for is a required step in ion-exchange chromatography. The packed column is equilibrated with a buffer. O Determination of the distance traveled by the analyte. O The column is washed with additional buffer to remove unbound components. O The elution of the bound analyte. O The sample is loaded onto the column.

Find the Rf value from given data. Thank you

Based on your value of Ksp1 what is the solubility of borax in the room temperature solution? Trial 1: 0.0141 T2: 0.015 AVG: 0.0139  Temp: 293.15K

Hello, the priority to be answered would be page 1 (1st image). However, I can put part 2 (2nd image) on a new question/post. Just wanted to include that in this post for reference to whoever will answer it. Thank you!

Suzy Baker What three areas are affected by an "irritant"? (refer to ACS Safety in the Academic Chemistry Laboratories Vol. 1)    [use the answer format= ans 1, ans 2, ans 3]

1) Which of the diet sodas have the highest concentration of caffeine? Which standard best reflects this concentration?

Table #2 Worktable for BCA Assay Standard Water (μl) Tube # 1 2 3 4 5 6 7 8 9 0 125 325 175 325 325 325 400 400 BSA source (μl) 300 stock 375 stock 325 stock 175 of tube #2 325 of tube #3 325 of tube #5 325 of tube #6 100 of tube #7 0 Protein (ug) Add the volumes listed in Table 2 to each tube. Add the water first and then the appropriate standard. Pipette each of the protein samples directly into the water, do not dribble on the side of the test tubes. The standard is at a concentration of 2 mg/ml and contains bovine serum albumin (BSA). Calculate the amount of BSA protein added to each of the tubes and write this in Table 2 (Protein (ug)).

Please refer to the image attached below and answer the following question. Thanks What referrals would you give to her and in what order?

match to the corresponding molecule

I. Look at the MS/MS for paracetamol (a drug), explain the peaks seen at 134 and 110, and draw the structure for the 110 peak. Paracetamòl N-(4-hydroxyphenyliacetamide ESI+, MS/MSs Kky pan_03_c80720211368 1-38 RT: 0.00024 AV 35 N: 107ES T TMSES F ms 12.00g24 00 0 00-10.0g 100g 110.0 CH но 70 (M.H]* 162.0 16 1340 130 100

. Be sure to understand what the dilutionfactor is for each sample (dilution factor = the number used to determine the actualconcentration of the stock UK after the diluted samples are analyzed) ?

Color BEFORE adding Color AFTER adding Intensity of color AFTER adding FeCl3 Test Tube # FeCl3 FeCl3 #1 (salicylic acid) clear lilac 100% #2 (commercial aspirin A) clear slightly pink 50% #3 (commercial aspirin B) clear lilac 95% #4 (aspirin from your clear pink 20% synthesis above) #5 (control) clear clear 0% Based on this data and the melting point of Aspirin, how pure is the synthesized product?

Table 1. Data on EDTA Standardization Weight of CaCO3 (g): 0.2003g Trial Volume of EDTA (mL) Molarity of EDTA (M) 1 8.60 2 8.50 8.55 Average Molarity of EDTA (M)

Using the data provided please help me answer this question.  Determine the concentration of the iron(Ill) salicylate in the unknown directly from to graph and from the best fit trend-line (least squares analysis) of the graph that yielded a straight line.