beer's law Report

In Beer's Law, when the absorbance of a solution is 0, this means that  a. all of the incident light passes through the solution and hits the dector b. all of the incident light is reflected by the solution, none hits the dector c. all of the incident light is absorbed by the solution, so it all hits the dector d. all of the incident light is absorbed by the solution, so none hits the dector

PLEASE show steps in your calculations

please can you please im begging can you please create graphs with Excel (or a similar program). Plot absorbance (v-axis) vs. A (×- axis) for the absorbance curve determined in part 2. Connect the points with a smooth curve. Plot absorbance (y-axis) vs. concentration (x-axis) for the Beer's Law data from part 3. Draw a best-fit line or use a program such as Excel and do a least-squares fit to determine the best-fit line through the points. please answer super super fast please please need it now its urgent just creat the graphs

Table II Cuvette # Concentration Absorbance 0.50 M 1 1.049 2 0.40 M 0.926 3 0.20 M 0.740 0.298 4 0.10 M 5 0.05 M 0.202 Unknown # 9 0.427 Unknown # 10 7 0.646 Blank (DI water) 0 1. If Beer's Law is valid, the value of "E" should be constant. Use A = E*C*L or E= A/(E*L) and the data from Table II to calculate values of E for the five solutions below The value of L is normally 1 cm so E = A/C in this experiment. Table III Solution # Calculated Values of E 1 2 3 4 5

in the images attach can you please calculate the Concentration of Allura Red M and can you also please please Create graphs with Excel (or a similar program). Plot absorbance (v-axis) vs. A (×- axis) for the absorbance curve determined in part 2. Connect the points with a smooth curve. Plot absorbance (y-axis) vs. concentration (x-axis) for the Beer's Law data from part 3. Draw a best-fit line or use a program such as Excel and do a least-squares fit to determine the best-fit line through the points. lastly can you please show a sample the concentration of the solutions made by dilution. Also use the slope of the best-fit line from your absorbance vs. concentration plot to calculate the concentration of Allura Red in each unknown. Also please dont forget to do the results box in the image attach please please answer super super fast i really need it its very urgent and important please answer everything i need it before the end of today before 11:59pm I would really appreciate it if…

Table 1. pH of water samples Sample No. Water Samples pH 1 Tap water from household 6.59 2 Water from creek 7.35 3 Water from river 7.35 4 Unknown sample 7.00 Table 2. Absorbance of Samples Table 3. Calibration Curve Data* Sample No. Absorbance Absorbance 3- Concentration of PO4 Ions, ppm (1 pt each) 1 1.33 1.0 0.050 2 3.26 2.0 0.093 0.89 3.0 0.137 4 0.099 4.0 0.185 5.0 0.230 *Provided by the instructor II. Graph Determination of Phosphate Concentration 3- 1. Using Excel, plot the concentration of PO4* (x-axis) vs. absorbance (refer to Table 3) The olot must show the equation of the best-fit (or trend) line of the graph. Attach the graph below

If a solution has an absorbance of 2 you can be certain that  the solution is highly diluted and needs to be concentrated  all the statements are correct  this is a reliable absorbance and downstream analysis can be done  the solution is highly concentrated and needs dilution

How did you decide on the wavelength at which you will read the absorbance of the diluted solutions? What is the importance of reading the absorbance of diluted solutions at λmax? What will vary if you chose a wavelength 20 nm lower or higher than your chosen wavelength? (Minimum of 8 sentences)

A. Copper sulfate pentahydrate is often used to teach students about Beer’s Law by making a standard curve (linear regression). The bright blue color of the solution has a peak absorbance in the red range of the electromagnetic spectrum. The absorbance value is proportional to concentration. How many grams of CuSO4 5H2O to make up 250 mL of a 0.2 M stock of this solution? B. Using M1V1 = M2V2 how many mL would a student need to measure out in graduated cylinder to make up a 50 mL (V2) solution of 0.1M, 0.05 M, 0.025M 0.0125M solutions for a standard curve at the peak wavelenth for this compound? I need help with part B of this question.

A. Copper sulfate pentahydrate is often used to teach students about Beer’s Law by making a standard curve (linear regression). The bright blue color of the solution has a peak absorbance in the red range of the electromagnetic spectrum. The absorbance value is proportional to concentration. How many grams of CuSO4 5H2O to make up 250 mL of a 0.2 M stock of this solution? B. Using M1V1 = M2V2 how many mL would a student need to measure out in graduated cylinder to make up a 50 mL (V2) solution of 0.1M, 0.05 M, 0.025M 0.0125M solutions for a standard curve at the peak wavelenth for this compound?

If A=absorbance, B=molar absorptivity, C=length of light path, D=concentration, and you plot a graph of absorbance (y-axis) vs concentration (x-axis), what does the slope of the Beer's Law (A=BCD) curve signify? Choose one:  a. absorbance b. molar absorptivity c. length of light path d. concentration

25. How would you set up and solve concentration problems like this which involve absorbance?

The protein content of the unknown samples were determined using BSA as standard. The absorbance values of the standard solutions and samples are as follows. BSA concentration (mg/mL) Absorbance 0.000 20 0.110 40 0.192 60 0.248 80 0.325 100 0.400 120 0.460 a. What is the equation of the line? b. Determine the protein concentration of samples in ug/mL. Sample dilutions 1 in 14 A540 0.150 1 in 5 1 in 8 0.380 0.235

When using a spectrophotometer to measure concentration of a solution, which of the following absorbance readings would indicate the highest concentration? 1. 0.56 2. 0.32 3. 1.34 4. 1.28

The best fit curve line to an absorbance vs. concentration plot for standard solutions of a dye has a slope of 0.1815 mL/mg and intercept of 0.0477. A sample solution prepared of the same dye has an absorbance of 0.464 at the wavelength used to establish the Beer's law plot. What is the concentration of dye in the prepared solution? Select one: O a. 0.132 mg/mL b. 2.29 mg/mL O c. 0.464 mg/mL O d. 2.82 mg/mL e. 0.0365 mg/mL Next page Law Tutorial Part 2 Jump to... Exp #9 - Laboratory Practical► Worksheet étv A

Riboflavin, also known as vitamin B2, has a molar absorptivity coefficient of 3 x 10 ^3 . If the absorbance reading at a Lmax = 350 nm is 0.90 using a 1 cm cell, what is the concentration of riboflavin in the sample. a. 3.0 x 10^-4 b. 8.57 c. 1.80 x 10^3 d. 9.45 x 10-5

You have prepared several dilutions of a standard compound. You measure the absorbance of each solution at 340 nm using a 1 cm cuvette and your results are listed in the table below. Determine the concentration of an unknown sample with an absorbance of 0.085 at 340 nm. Concentration (uM) Absorbance at 340 nm 2. 0.009 4. 0.020 0.061 16 0.111 32 0.189

please please im begging please please answer images attached super super super fast i really need it before 11:40pm today please answer fast i would really appreciate it

em1.png chem3.png Part 3: Q9 Average absorbance Flask 8 Flask 9 Flask 10 Flask 11 Part 3: Q9 Flask 8 Flask 9 Flask 10 Flask 11 Part 3: Q10 Flask 8 Flask 9 Flask 10 Flask 11 Q Part 3 0.146 [Fe] -0.002mol/L 0.166 volume 5.00mL 0.215 0.294 [FESCN mol/L) ✪ 4.59* 10 5.18*10* 6.64 x 10 9.08*10* [Fe(mol/L) 5.00 x 10 5.00*10* Y=3362x -0.0083 R¹ 0.9993 5.00 x 10 5.00*10* (Amounts of flask 8 (9 - 11's values are in the spreadsheet) 1:1 Ratio so, Molarity of Fe(NO3))3 = 0.2M Volume of Fe(NO3))3 = 5.00mL 71% Moles of Fe³= Molarity x volume(L) Moles of Fe = 0.2M × 0.005L = 1× 10-³mole 68% [SCN ](mol/ 4 x 10-4 5 x 10-4 7*10* 1*10* الخا chem2.png Concentration of Fe³ 9, X = mole of Fe³1x10³ mole Volume 0.0054 Ⓒ 8, Average absorbance for solution 8 (same for 9 - 11) Average absorbance for flask 8 = sum of all absorbances / number of readings (0.145 +0.147 +0.148) 3 Using equation of calibration curve's line(slope) of best fit, the average absorbances of the unknown concentrations to determine…

10. The concentration of haemoglobin in a blood sample was determined by spectrophotometry. A standard curve of the absorbance at 412 nm of several solutions of known haemoglobin concentrations was created. The data for the standard curve is shown below. a. Calculate the linearity coefficient (r), y-intercept and slope. b. What is the concentration (in ug/mL) of haemoglobin in your sample if the absorbance obtained at 412 nm was 0.303? Absorbance Concentration of standard solution (pg/ml) (412nm) 0.069 1 0.113 2 0.201 4 0.377 8. 0.730 16

P 1. Absorbance Given the following standard curve of Absorbance vs Concentration (mM), what in the concentration of Aspirin if the absorbance is O.822? 1.2 y = 0.0032x+0.1043 R2 0.9975 0.8 0.6 0.4 0.2 250 000 350 Concentration MacBook Air 08 ODO F4 110 F8 F5 DD 93 63 6. R.

When using cuvettes to measure the absorbance of a solution, why is it necessary to only hold the cuvettes near the top?