Protein Extraction and Folding Lab report

docx

School

Marshall University *

*We aren’t endorsed by this school

Course

212

Subject

Chemistry

Date

Feb 20, 2024

Type

docx

Pages

5

Uploaded by MegaField2834

Report
Protein Extraction and Folding Alisha Varney 08/30/2023 CHM 218 Sec 104 Bill Price
I. Introduction In this experiment the protein Spirulina is tested using a buffer, to see under what conditions the protein will fold and denature. First the protein was mixed with sand and separated into 5 test tubes for five experiments before the buffer was added. Then multiple substances and environmental factors were tested to see what would denature the protein. The goal was to determine under what circumstances that spirulina would fold. II. Data Test tube #1: 1. Sand, buffer, and protein mixture is prepared. 2. Water is heated to 74.7 on hot plate. ℃ 3. Test-tube is inserted into water for 1 minute. 4. The red fluoresce of protein was gone and the solution turned a dark shade of green, therefore it was denatured by heat. Test Tube #2 1. Sand, buffer, and protein mixture is prepared. 2. 3 drops 0.1m NaCl added to mixture. 3. Protein was not denatured. Test Tube #3 1. Sand, buffer, and protein mixture is prepared. 2. 1 pump of dish soap added to mixture. 3. Protein was denatured and turned green. Test Tube #4 1. Sand, buffer, and protein mixture is prepared. 2. 3 drops NaOH 1.0m added to mixture. 3. Protein was denatured and turned light green. Test Tube #5 1. Sand, buffer, and protein mixture is prepared. 2. 4 pumps of acetone were added to mixture. 3. Protein did not denature.
III. Examples Calculations No calculations for this lab IV. Results and Discussion It was found that temperature increase, dish soap, and NaOH all are things that denatured the tested protein. This would be because the chemical make-up of these components has protein interactions that are stronger than the intermolecular forces of the protein, causing it to fold. In the same experiment it was concluded that acetone and NaCl could not denature the protein. This is because they do not have any protein interactions and proteins are insoluble in these components. All the components tested behaved as expected by chemical reactions, given that the protein solubility and intermolecular forces did or did not affect the protein and cause it to fold. In conclusion, it was found that heat and protein soluble substances could denature the protein while non protein soluble substances with weak forces could not denature the protein. V. Questions Pre Lab- 1. Structurally all amino acids consist of a hydrogen atom, -H, a carboxylic acid group, -COOH, and an amine group, -NH2, attached to a central carbon atom. amino acids differ from each other by nature of the R group. Shown below are the amino acids serine and isoleucine. Classify the R group in each amino acid as polar or non-polar. Provide and brief explanation. General Amino acid structure= Non-polar, no Nitrogen in the R group Serine= Polar, Carbon and Nitrogen in the R group Isoleucine= Polar, Carbon and Nitrogen in the R group 2. Describe hydrogen bonding and how it influences protein structure. A hydrogen bond is a type of dipole-dipole interaction between a hydrogen and an electronegative atom (O2, N, or Florine). These bonds influence protein structure by forming between amino acids that hold the protein in its shape. 3. Explain what is meant by the following terms: primary, secondary, tertiary, and quaternary- structure of a protein. Primary= strait amino acid sequence poly-peptide chain
Your preview ends here
Eager to read complete document? Join bartleby learn and gain access to the full version
  • Access to all documents
  • Unlimited textbook solutions
  • 24/7 expert homework help
Secondary= sequence forms into a helix/ sheet that make up the backbone of the protein Tertiary= 3D structure of a whole polypeptide chain Quaternary= 3d arrangement of subunits of a proteins entire structure. 4. Proteins exhibit colloidal characteristics in solution. a. What is a colloid? A mixture of 2 substances that do not completely combine. b. What simple test can distinguish a colloid from and solution? The Tyndall Effect Post Lab- 1. What is the role of the phosphate buffer in stabilizing the native structure of the phycocyanin. The buffer stops big changes in the solutions PH that prevent denaturing. 2. Find the structures for acetone, urea, and other reagents used in this lab. Discuss the interactions these molecules can have with both water and the protein. How does each of these affect the folding state? Use the nature of the intermolecular forces to explain. Acetone- CH3-C-CH3, can form H-bonds, but has no protein interaction and therefore will not fold the protein because proteins are insoluble in acetone. Urea- O , Urea is soluble in water and will break protein bonds, || causing them to fold. C / \ H2N NH2 . . NaCl- Na. :Cl: , NaCl is soluble in water and increases protein solubility which is why the proteins folded when it was added to the solution.
3. Develop a hypothesis to explain why the protein is folded in its native state. A protein will fold in its native state depending on its environment, such as temperature change, and if it is experiencing polar or nonpolar molecules in its environment. 4. A protein contains an amino acid with COOO- side chain group. Would this amino acid be more likely found on the interior or exterior of a protein in its native structure? Why? The amino acid would most likely be found on the exterior of the protein because it will have polar interactions with water, given it is hydrophilic. 5. Draws the Lewis structure of acetone and answer the following. H O H | | | H-C-C-C-H | | H H a. Which if any H-atoms in acetone can participate in hydrogen bonding? None b. Which if any non-H-atoms in acetone can participate in hydrogen bonding? None, hydrogen bonding requires an atom of Hydrogen(H) c. Is acetone capable of forming hydrogen bonds? If yes, does acetone function as a hydrogen donor, a hydrogen acceptor, or both? Explain. No, it cannot form a hydrogen bond because H is not Bonded directly to O.

Related Documents

lab 17 (1).docx.pdf
CHM 142 Exam 1A F23 key-revised-12-11-23.pdf
SITHASC024 Class activity book - Student_012023.docx
Chem1011GeneralChemistryII_EquilibriumandLeChteliersPrinciple_134692.pdf
Chem1011GeneralChemistryII_TitrationforAceticAcidinVinegar_134692.pdf
CH5-CH8.rtf
beer's law Report.docx
CHEM101L Lab 5 F.pdf
CHEM101L Lab 6 F.pdf
CHEM101L Lab 7 F.pdf
OCHEM Lab report 1.docx
CHEM101L Lab 4 F.pdf
Recommended textbooks for you
Text book image
Chemistry
Chemistry
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Cengage Learning
Text book image
Chemistry: An Atoms First Approach
Chemistry
ISBN:9781305079243
Author:Steven S. Zumdahl, Susan A. Zumdahl
Publisher:Cengage Learning
Text book image
Introductory Chemistry: A Foundation
Chemistry
ISBN:9781337399425
Author:Steven S. Zumdahl, Donald J. DeCoste
Publisher:Cengage Learning
Text book image
Chemistry: Matter and Change
Chemistry
ISBN:9780078746376
Author:Dinah Zike, Laurel Dingrando, Nicholas Hainen, Cheryl Wistrom
Publisher:Glencoe/McGraw-Hill School Pub Co
Text book image
General Chemistry - Standalone book (MindTap Cour...
Chemistry
ISBN:9781305580343
Author:Steven D. Gammon, Ebbing, Darrell Ebbing, Steven D., Darrell; Gammon, Darrell Ebbing; Steven D. Gammon, Darrell D.; Gammon, Ebbing; Steven D. Gammon; Darrell
Publisher:Cengage Learning
Text book image
Chemistry: The Molecular Science
Chemistry
ISBN:9781285199047
Author:John W. Moore, Conrad L. Stanitski
Publisher:Cengage Learning
SEE MORE TEXTBOOKS
Recommended textbooks for you
Text book image
Chemistry
Chemistry
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Cengage Learning
Text book image
Chemistry: An Atoms First Approach
Chemistry
ISBN:9781305079243
Author:Steven S. Zumdahl, Susan A. Zumdahl
Publisher:Cengage Learning
Text book image
Introductory Chemistry: A Foundation
Chemistry
ISBN:9781337399425
Author:Steven S. Zumdahl, Donald J. DeCoste
Publisher:Cengage Learning
Text book image
Chemistry: Matter and Change
Chemistry
ISBN:9780078746376
Author:Dinah Zike, Laurel Dingrando, Nicholas Hainen, Cheryl Wistrom
Publisher:Glencoe/McGraw-Hill School Pub Co
Text book image
General Chemistry - Standalone book (MindTap Cour...
Chemistry
ISBN:9781305580343
Author:Steven D. Gammon, Ebbing, Darrell Ebbing, Steven D., Darrell; Gammon, Darrell Ebbing; Steven D. Gammon, Darrell D.; Gammon, Ebbing; Steven D. Gammon; Darrell
Publisher:Cengage Learning
Text book image
Chemistry: The Molecular Science
Chemistry
ISBN:9781285199047
Author:John W. Moore, Conrad L. Stanitski
Publisher:Cengage Learning
SEE MORE TEXTBOOKS