PhysioEx Exercise 1 Activity 1

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American River College *

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Chemistry

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Feb 20, 2024

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9/7/23, 2 : 53 PM PhysioEx Exercise 1 Activity 1 Page 1 of 4 https://media.pearsoncmg.com/bc/bc_0media_ap/physioex/10/ex1/act1/ PhysioEx Lab Report Exercise 1: Cell Transport Mechanisms and Permeability Activity 1: Simulating Dialysis (Simple Di ff usion) Name: So fi a Marquez Date: 7 September 2023 Session ID: session-df21f3a8-5b3a-26ad-030c-65072e5dbaae Pre-lab Quiz Results You scored 100% by answering 3 out of 3 questions correctly. Experiment Results Predict Questions Stop & Think Questions The driving force for di ff usion is You correctly answered: the kinetic energy of the molecules in motion. 1 In di ff usion, molecules move You correctly answered: from high concentration to low concentration. 2 Which of the following dialysis membranes has the largest pore size? You correctly answered: 200 MWCO. 3 Predict Question 1: The molecular weight of urea is 60.07. Do you think urea will di ff use through the 20 MWCO membrane? Your answer: No, not at all. 1 Predict Question 2: Recall that glucose is a monosaccharide, albumin is a protein with 607 amino acids, and the average molecular weight of a single amino acid is 135 g/mol. Which of the following will be able to di ff use through the 200 MWCO membrane? Your answer: neither glucose nor albumin. 2
9/7/23, 2 : 53 PM PhysioEx Exercise 1 Activity 1 Page 2 of 4 https://media.pearsoncmg.com/bc/bc_0media_ap/physioex/10/ex1/act1/ Experiment Data Solute MWCO Left Solute (mM) Right Solute (mM) Average Di ff usion (mM/sec) Na Cl 20 9.00 0.00 0.0000 Urea 20 0.00 0.00 0.0000 Albumin 20 0.00 0.00 0.0000 Glucose 20 0.00 0.00 0.0000 Na Cl 20 0.00 0.00 0.0000 Urea 20 9.00 0.00 0.0000 Albumin 20 0.00 0.00 0.0000 Glucose 20 0.00 0.00 0.0000 Na Cl 50 9.00 0.00 0.0150 Urea 50 0.00 0.00 0.0000 Albumin 50 0.00 0.00 0.0000 Glucose 50 0.00 0.00 0.0000 Na Cl 50 18.00 0.00 0.0273 Urea 50 0.00 0.00 0.0000 Albumin 50 0.00 0.00 0.0000 Glucose 50 0.00 0.00 0.0000 Na Cl 100 9.00 0.00 0.0150 Urea 100 0.00 0.00 0.0000 The reason sodium chloride didn't di ff use left to right is that You correctly answered: the membrane pore size was too small. 1 Glucose is a six-carbon sugar. Albumin is a protein with 607 amino acids. The average molecular weight of a single amino acid is 135 g/mol. There is no reason to run these solutes at the 20 MWCO because You correctly answered: glucose and albumin are both too large to pass. 2 The rate of di ff usion for urea You correctly answered: is slower than that for sodium because urea is a larger molecule. 3 + - + - + - + - + -
9/7/23, 2 : 53 PM PhysioEx Exercise 1 Activity 1 Page 3 of 4 https://media.pearsoncmg.com/bc/bc_0media_ap/physioex/10/ex1/act1/ Albumin 100 0.00 0.00 0.0000 Glucose 100 0.00 0.00 0.0000 Na Cl 100 0.00 0.00 0.0000 Urea 100 9.00 0.00 0.0094 Albumin 100 0.00 0.00 0.0000 Glucose 100 0.00 0.00 0.0000 Na Cl 200 0.00 0.00 0.0000 Urea 200 0.00 0.00 0.0000 Albumin 200 0.00 0.00 0.0000 Glucose 200 9.00 0.00 0.0042 Na Cl 200 0.00 0.00 0.0000 Urea 200 0.00 0.00 0.0000 Albumin 200 9.00 0.00 0.0000 Glucose 200 0.00 0.00 0.0000 Na Cl 20 0.00 0.00 0.0000 Urea 20 9.00 0.00 0.0000 Albumin 20 0.00 0.00 0.0000 Glucose 20 0.00 0.00 0.0000 Post-lab Quiz Results You scored 100% by answering 4 out of 4 questions correctly. + - + - + - + - The e ff ect of increasing the concentration of sodium chloride from 9 mM to 18 mM in the left beaker was to You correctly answered: increase the rate of di ff usion. 1 Describe the di ff erence between the rate of di ff usion seen for sodium and urea. You correctly answered: Urea di ff used more slowly because it is larger than sodium. 2 Which of the following solutes did not pass through any of the membranes? You correctly answered: albumin. 3
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9/7/23, 2 : 53 PM PhysioEx Exercise 1 Activity 1 Page 4 of 4 https://media.pearsoncmg.com/bc/bc_0media_ap/physioex/10/ex1/act1/ Review Sheet Results When di ff usion stops, we say the solution has reached You correctly answered: equilibrium. 4 Describe two variables that a ff ect the rate of di ff usion. Your answer: The concentration of the solute and MWCO membrane. 1 Why do you think the urea was not able to di ff use through the 20 MWCO membrane? How well did the results compare with your prediction? Your answer: The reason urea could not di ff use through the 20 MWCO membrane was that the molecular weight was too high to di ff use that membrane. The results were aligned with my prediction since urea was unable to di ff use. 2 Describe the results of the attempts to di ff use glucose and albumin through the 200 MWCO membrane. How well did the results compare with your prediction? Your answer: Glucose was the only one that was able to pass through the 200 MWCO membrane, but albumin was not able to. I predicted that neither one would be able to pass through, but glucose was able to. While my prediction that albumin would not be able to pass through was correct. 3 Put the following in order from smallest to largest molecular weight: glucose, sodium chloride, albumin, and urea. Your answer: Sodium chloride, urea, glucose, and albumin. 4