PhysioEx Exercise 1 Activity 1
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:
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PhysioEx Exercise 1 Activity 1
Page 1 of 4
https://media.pearsoncmg.com/bc/bc_0media_ap/physioex/10/ex1/act1/
PhysioEx Lab Report
Exercise 1: Cell Transport Mechanisms and Permeability
Activity 1: Simulating Dialysis (Simple Di
ff
usion)
Name: So
fi
a Marquez
Date: 7 September 2023
Session ID: session-df21f3a8-5b3a-26ad-030c-65072e5dbaae
Pre-lab Quiz Results
You scored 100% by answering 3 out of 3 questions correctly.
Experiment Results
Predict Questions
Stop & Think Questions
The driving force for di
ff
usion is
You correctly answered: the kinetic energy of the molecules in motion.
1
In di
ff
usion, molecules move
You correctly answered: from high concentration to low concentration.
2
Which of the following dialysis membranes has the largest pore size?
You correctly answered: 200 MWCO.
3
Predict Question 1: The molecular weight of urea is 60.07. Do you think urea will di
ff
use
through the 20 MWCO membrane?
Your answer: No, not at all.
1
Predict Question 2: Recall that glucose is a monosaccharide, albumin is a protein with 607
amino acids, and the average molecular weight of a single amino acid is 135 g/mol.
Which of the following will be able to di
ff
use through the 200 MWCO membrane?
Your answer: neither glucose nor albumin.
2
9/7/23, 2
:
53 PM
PhysioEx Exercise 1 Activity 1
Page 2 of 4
https://media.pearsoncmg.com/bc/bc_0media_ap/physioex/10/ex1/act1/
Experiment Data
Solute
MWCO
Left Solute
(mM)
Right Solute
(mM)
Average
Di
ff
usion
(mM/sec)
Na
Cl
20
9.00
0.00
0.0000
Urea
20
0.00
0.00
0.0000
Albumin
20
0.00
0.00
0.0000
Glucose
20
0.00
0.00
0.0000
Na
Cl
20
0.00
0.00
0.0000
Urea
20
9.00
0.00
0.0000
Albumin
20
0.00
0.00
0.0000
Glucose
20
0.00
0.00
0.0000
Na
Cl
50
9.00
0.00
0.0150
Urea
50
0.00
0.00
0.0000
Albumin
50
0.00
0.00
0.0000
Glucose
50
0.00
0.00
0.0000
Na
Cl
50
18.00
0.00
0.0273
Urea
50
0.00
0.00
0.0000
Albumin
50
0.00
0.00
0.0000
Glucose
50
0.00
0.00
0.0000
Na
Cl
100
9.00
0.00
0.0150
Urea
100
0.00
0.00
0.0000
The reason sodium chloride didn't di
ff
use left to right is that
You correctly answered: the membrane pore size was too small.
1
Glucose is a six-carbon sugar. Albumin is a protein with 607 amino acids. The average
molecular weight of a single amino acid is 135 g/mol. There is no reason to run these
solutes at the 20 MWCO because
You correctly answered: glucose and albumin are both too large to pass.
2
The rate of di
ff
usion for urea
You correctly answered: is slower than that for sodium because urea is a larger molecule.
3
+
-
+
-
+
-
+
-
+
-
9/7/23, 2
:
53 PM
PhysioEx Exercise 1 Activity 1
Page 3 of 4
https://media.pearsoncmg.com/bc/bc_0media_ap/physioex/10/ex1/act1/
Albumin
100
0.00
0.00
0.0000
Glucose
100
0.00
0.00
0.0000
Na
Cl
100
0.00
0.00
0.0000
Urea
100
9.00
0.00
0.0094
Albumin
100
0.00
0.00
0.0000
Glucose
100
0.00
0.00
0.0000
Na
Cl
200
0.00
0.00
0.0000
Urea
200
0.00
0.00
0.0000
Albumin
200
0.00
0.00
0.0000
Glucose
200
9.00
0.00
0.0042
Na
Cl
200
0.00
0.00
0.0000
Urea
200
0.00
0.00
0.0000
Albumin
200
9.00
0.00
0.0000
Glucose
200
0.00
0.00
0.0000
Na
Cl
20
0.00
0.00
0.0000
Urea
20
9.00
0.00
0.0000
Albumin
20
0.00
0.00
0.0000
Glucose
20
0.00
0.00
0.0000
Post-lab Quiz Results
You scored 100% by answering 4 out of 4 questions correctly.
+
-
+
-
+
-
+
-
The e
ff
ect of increasing the concentration of sodium chloride from 9 mM to 18 mM in the
left beaker was to
You correctly answered: increase the rate of di
ff
usion.
1
Describe the di
ff
erence between the rate of di
ff
usion seen for sodium and urea.
You correctly answered: Urea di
ff
used more slowly because it is larger than sodium.
2
Which of the following solutes did not pass through any of the membranes?
You correctly answered: albumin.
3
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9/7/23, 2
:
53 PM
PhysioEx Exercise 1 Activity 1
Page 4 of 4
https://media.pearsoncmg.com/bc/bc_0media_ap/physioex/10/ex1/act1/
Review Sheet Results
When di
ff
usion stops, we say the solution has reached
You correctly answered: equilibrium.
4
Describe two variables that a
ff
ect the rate of di
ff
usion.
Your answer:
The concentration of the solute and MWCO membrane.
1
Why do you think the urea was not able to di
ff
use through the 20 MWCO membrane?
How well did the results compare with your prediction?
Your answer:
The reason urea could not di
ff
use through the 20 MWCO membrane was that the molecular weight was too high to di
ff
use that membrane. The results were aligned with my prediction since urea was unable to di
ff
use.
2
Describe the results of the attempts to di
ff
use glucose and albumin through the 200
MWCO membrane. How well did the results compare with your prediction?
Your answer:
Glucose was the only one that was able to pass through the 200 MWCO membrane, but albumin was not able to. I predicted that neither one would be able to pass through, but glucose was able to. While my prediction that albumin would not be able to pass through was correct.
3
Put the following in order from smallest to largest molecular weight: glucose, sodium
chloride, albumin, and urea.
Your answer:
Sodium chloride, urea, glucose, and albumin.
4
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