Diffusion Workbook

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Coastal Carolina Community College *

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Course

110

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Chemistry

Date

Feb 20, 2024

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docx

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5

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Diffusion TOTAL POINTS: 24 POINTS PRE-LAB QUESTIONS (3 POINTS) 1. A concentration gradient affects the direction that solutes diffuse. Describe how molecules move with respect to concentration. a. Diffusion of solutions typically move from areas of high to low concentration. 2. How does the size of a solute affect the rate of diffusion? a. Solute diffusion and size are inversely proportional, the bigger the solute is the slower it diffuses. 3. Does polarity affect the rate of diffusion? Explain your answer. a. Polarity has little effect on the rate of diffusion. Polar molecules do not fuse well with the lipid core of the phospholipid bilayer of the plasma membrane. Nonpolar molecules diffuse much easier. ©eScience Labs, 2018
Diffusion EXPERIMENT 1: DIFFUSION THROUGH A LIQUID (5 POINTS) Result Tables Table 1: Rate of Diffusion in Corn Syrup Time (sec) Blue Dye Red Dye 10 4 cm 4 cm 20 2.5 cm 2 cm 30 2 cm 1.7 cm 40 1.9 cm 1.5 cm 50 1.5 cm .5 cm 60 1.4 cm .4 cm 70 1.4 cm .3 cm 80 1.4 cm .3 cm 90 1.3 cm .2 cm 100 1.2 cm .2 cm 110 1.2 cm .1 cm 120 1 cm .1 cm ©eScience Labs, 2018
Diffusion Post-Lab Questions (4 points) 1. Write a hypothesis for this experiment. a. The red dye should diffuse faster than the blue dye because it is lighter in weight. 2. Which dye diffused the fastest? a. The red dye. 3. Does the rate of diffusion correspond with the molecular weight of the dye? a. Yes because as the solution diffused, it became less concentrated and diffused slower. Both were rapid in the beginning because of the high concentration. 4. Does the rate of diffusion change over time? Why or why not? a. The rate of diffusion does change over time; initially, it is rapid due to the high concentration, but as the solution becomes less concentrated, diffusion slows down. ©eScience Labs, 2018
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Diffusion EXPERIMENT 2: CONCENTRATION GRADIENTS AND MEMBRANE PERMEABILITY (5 POINTS) Result Tables Table 3: Indicator Reagent Data Indicator Starch Positive Control (Color) Starch Negative Control (Color) Glucose Positive Control (Color) Glucose Negative Control (Color) IKI Solution Dark brown Dark yellow n/a n/a Glucose Test Strip n/a n/a Yellow Yellow Table 4: Diffusion of Starch and Glucose Over Time Indicator Dialysis Bag After 1 Hour Beaker Water After 1 Hour IKI Solution Yellow with brown swirl Dark yellow Glucose Test Strip Yellow Yellow Post-Lab Questions (5 Points) 1. Why is it necessary to have positive and negative controls in this experiment? a. Because it ensures that the results are due to independent variables. 2. Draw a diagram of the experimental set-up. Use arrows to depict the movement of each substance in the dialysis bag and the beaker. a. 3. Which substance(s) crossed the dialysis membrane? Support your response with data- based evidence. ©eScience Labs, 2018
Diffusion a. The glucose did pass through based off the leftover starch in the bag. 4. Which molecules remained inside of the dialysis bag? a. The starch molecules. 5. Did all of the molecules diffuse out of the bag into the beaker? Why or why not? a. No, there were remnants of starch left in the bag. PICTURE (2 POINTS): Insert a picture of yourself completing this lab to this word document prior to uploading for full credit. ©eScience Labs, 2018