PhysioEx Exercise 8 Activity 4

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University of Cincinnati, Clermont College *

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1015L

Subject

Chemistry

Date

Jun 14, 2024

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pdf

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3

Uploaded by MasterFog13529

PhysioEx Lab Report Exercise 8: Chemical and Physical Processes of Digestion Activity 4: Assessing Lipase Digestion of Fat Name: Chrissy Alden Date: 9 June 2024 Session ID: session-e79d541d-5f31-9249-6345-ba62daf1bf10 Pre-lab Quiz Results You scored 100% by answering 4 out of 4 questions correctly. Experiment Results Predict Question Stop & Think Questions Which of the following is/are true of bile? You correctly answered: It works by a physical process. 1 The substrate used in this simulation is You correctly answered: vegetable oil. 2 When fatty acids are liberated by lipase, the pH You correctly answered: decreases. 3 One of the products of the chemical digestion of lipids is You correctly answered: fatty acids. 4 Predict Question: Which tube do you think will have the highest lipase activity? Your answer: tube 3 (lipase, deionized water, bile salts, pH 9.0 buffer). 1 What does the pH measure? You correctly answered: both lipase activity and fatty acid release. 1 Why is it difficult to detect whether lipase is active in tube 5 (lipase, vegetable oil, bile salts, pH 2.0 buffer)? You correctly answered: The pH is already very low, so a decrease in pH might be difficult to detect. 2
Experiment Data Tube No Reagent 1 Reagent 2 Reagent 3 Reagent 4 Time (min) Temp (°C) pH 1 Lipase Vegetable Oil Bile salts pH 7.0 Buffer 60 37 6.21 2 Lipase Vegetable Oil Deionized Water pH 7.0 Buffer 60 37 7.00 3 Lipase Deionized Water Bile salts pH 9.0 Buffer 60 37 9.00 4 Deionized Water Vegetable Oil Bile salts pH 7.0 Buffer 60 37 7.00 5 Lipase Vegetable Oil Bile salts pH 7.0 Buffer 60 37 7.00 6 Lipase Vegetable Oil Bile salts pH 9.0 Buffer 60 37 9.00 Post-lab Quiz Results You scored 100% by answering 4 out of 4 questions correctly. Review Sheet Results What is the product of lipase hydrolysis? You correctly answered: fatty acids. 1 From your results, which pH is ideal for pancreatic lipase digestion? You correctly answered: pH 7.0. 2 Which tube confirms that there is no lipase in bile salts or vegetable oil? You correctly answered: tube 4 (deionized water, vegetable oil, bile salts, pH 7.0 buffer). 3 Where in the body does pancreatic lipase hydrolyze triglycerides? You correctly answered: small intestine. 4 Explain why you can't fully test the lipase activity in tube 5 (lipase, vegetable oil, bile salts, pH 2.0 buffer). Your answer: By measuring the lipase, the pH would lower even more in the already low pH in tube 5. 1
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