BIO201 Lab 2

Lab 2 Cell Structure and Function BIO201L Student Name: MA ERICKA RENEE SAUVE Access Code (located on the lid of your lab kit): 2XVADW Lab Report Format Expectations Utilize college level grammar and professional formatting when completing this worksheet. Submissions without proper formatting, all required photos or sufficient responses will be rejected. Pre- lab Questions 1. Identify the major similarities and differences between prokaryotic and eukaryotic cells. Both cells have DNA but the DNA of Eukaryotic cell is found in nucleu while that of prokaryotes is found in the cytoplasm Eukaryotes contain Membrane bound organelles while Prokaryotes don't have these Both eukaryotes and prokaryotes have cell membrane and cell wall which is absent in animal cell present in plant cell but composition is different from that of prokaryotes Both have ribsomes but Eukaryotes have Ribosomes of 80s while prokaryotes have ribsomes of 70S . 2. Where is the DNA housed in a prokaryotic cell? Where is it housed in a eukaryotic cell? In prokaryotes Cytoplasm is place where DNA is found While in Eukaryotes it is found in Nucleus which is double membrane bound oragenelle. 3. Name three structures which provide support and protection in a eukaryotic cell. 1. CYTOSKELETON 2 Cell wall 3. Cell memane . CYOPLASM

Lab 2 Cell Structure and Function BIO201L EXPERIMENT 1: CELL STRUCTURE AND FUNCTION Introduction Questions 1. What is the difference between the rough and smooth endoplasmic reticulum? Rough endoplasmic Reticulum(RER) contains Ribosmes and functions in synthesis of Protien which is being transported outside cell Smooth endoplasmic reticulum do not contain ribosomes and it functions mainly in lipid metabolism and detixfying toxins. 2. Would an animal cell be able to survive without a mitochondria? Why or why not? No Anima cells would not survive with mitochondria as Animal needs energy to survive and Mitochondria are only organelles which functions to provide energy 3. What is the function of a lysosome? Lysosomes contains digesting enzymes so its function is digest and degrade bigger molecules and substance that enter cells and it also eat worn out organelles of cell by process known as Autophaggy.

Lab 2 Cell Structure and Function BIO201L EXPERIMENT 2: EXPLORING CELL SIZE Introduction Questions 1. State the surface area equation used in this experiment. Use an equation editor to insert this equation below, using correct mathematical formatting to include symbols, fractions and superscripts. Your response must be formatted correctly for credit . Surface area = 4πr^2 2. State the volume equation used in this experiment. Use an equation editor to insert this equation below, using correct mathematical formatting to include symbols, fractions and superscripts. Your response must be formatted correctly for credit . Volume= 4/3 πr^3 3. If you were to double the size of a cell, how would its surface area and volume change? Use the two equations you stated above to discuss this. ( Hint : Think of which of these two terms grows faster with an increase in radius.) Surface area will be 4 times and volum will be 6 times larger

Lab 2 Cell Structure and Function BIO201L Data and Observations In the table below, input the radius you selected with the dial in the experiment. (Note, the experiment will report the diameter, but the table requires the radius.) For each of the radii, calculate the values in the table and record the observed “Time to Center of Cell” that you observed. Table 1: Surface Area and Volume in Relation to Cell Size Radius (µm) Surface Area (µm 2 ) Volume (µm 3 ) Surface Area:Volume Ratio Time to Center of the Cell (s) 15 2827.43 14137.16 0.20 0.075 30 11309.16 28137.10 0.1 0.15 45 25446.90 381703.50 0.067 0.225 60 45238.93 904777.79 0.05 0.3 75 70685.83 1767144.8 0.04 0.375 90 101787.60 3053628.30 0.03 0.45 105 138544.23 4849048.30 0.028 0.525 120 180955.73 7232829.29 0.025 0.6 135 229022.10 10305994.70 0.022 0.675 150 282743.3 14137166.94 0.02 0.75

Lab 2 Cell Structure and Function BIO201L area to volume ratio causes more efficient absorption of nutrients and excretion of wastes. With the growth of cell volume of cell increase at greater rate than surface area which leads to reduced in surface area to volume ratio. This changes reduces the cells ability to take nutrient and excrete wasts at efficient rate. That is the reason Cell adopt a complex shape which increases surface area without significant changes in volume. .