BIO250 Lab 4 pdff123

pdf

School

Rasmussen College, Florida *

*We aren’t endorsed by this school

Course

G282/MCB22

Subject

Biology

Date

Jan 9, 2024

Type

pdf

Pages

Uploaded by MasterWaspMaster1018

Lab 4 Selective Media & Agar BIO250L ” Student Name: Brandon Estelle Access Code (located on the lid of your lab kit): ac-gif4su Lab Report Format Expecta0ons U"lize college level grammar and professional forma4ng when comple"ng this worksheet. Submissions without proper forma4ng, all required photos or suﬃcient responses will be rejected. Pre-lab Ques>ons 1. In this lab, you will conduct three different experiments that each cover the topic of selecAve media and agar. How are these experiments different from one another? Explain how the different media in each experiment are uAlized in the context of selecAve vs differenAal media. Click here to enter text. 2. What is the difference between chemically defined and chemically complex media? Give an example (a clinical or environmental research applicaAon) of when you would use each type of medium to culture different microorganisms. Chemically defined media is known chemical composiAon while chemically complex contains unknown chemical composiAon. 3. Why do we grow microorganisms on general growth media first, before inoculaAng differenAal media? To have a pure isolated colony that is able to grow without a medium affecAng growth, 4. What changes might you see on a differenAal plate that helps idenAfy the bacteria? Gram staining

Lab 4 Selective Media & Agar BIO250L ” EXPERIMENT 1: BIOPROSPECTING FOR STARCH DEGRADING BACTERIA Introductory Ques>ons 1. What results do you expect to see in Experiment 1 if your selected colony is amylase-producing? Small round spots of bacteria growth 2. What are some other potenAal sources of starch-degrading bacteria? Food, paper, soil, fermentaAon 3. Why did you perform Step 10, under the Prepare Stock SoluAon secAon, in this experiment? To weigh porAons by measuring appropriate volume 4. What kind of media did you use iniAally, and why? Starch agar because it contains bacteria that digests starches

Lab 4 Selective Media & Agar BIO250L ” Data and Observa>ons In the table below, report your observed growth corresponding to each plate. Denote whether there was growth on the starch plate, whether or not clear areas formed around the colonies that grew. Table 1: Starch Agar and Gram Iodine Results Sample Growth on Starch Agar? (Yes/N) Clear area around colonies? (Y/N) A Yes No B Yes No C Yes Yes D Yes Yes

Your preview ends here

Eager to read complete document? Join bartleby learn and gain access to the full version

Access to all documents
Unlimited textbook solutions
24/7 expert homework help

Lab 4 Selective Media & Agar BIO250L ” Provide a clear, high resoluAon photo of your plates aWer incubaAon with the lids removed. For credit on this lab, your photo must show the growth that is reported in your tabulated data, must depict the correct media with the iodine added and must also include your handwriXen name in the background. Plate A Plate B Plate C Plate D

Lab 4 Selective Media & Agar BIO250L ”

Lab 4 Selective Media & Agar BIO250L ” Results and Discussion 1. Describe the results of your starch test. Color changed and made it darker 2. Do the plates contain starch degrading bacteria? How do you know? Yes cow manure is starch degrading bacteria EXPERIMENT 2: SELECTION AND DIFFERENTIATION OF GRAM POSITIVE BACTERIA Introductory Ques>ons 1. What are the objecAves of Experiment 2? What media will you use? How does this media pertain to the topic of selecAvity? Agar to see bacteria growth 2. What results do you expect to see from Experiment 2 if the microbes are non-fermenters? What if they are fermenters? 3. What chemical in MSA confers selecAvity? How? It’s selecAve because sodium chloride has a high salt. ConcentraAon and bacteria cannot grow in these condiAons. 4. What component(s) makes MSA differenAal? How? Mannitol used for fermentaAon 5. Why are the nutrient agar plates used in this experiment? Allows growth of bacteria

Your preview ends here

Eager to read complete document? Join bartleby learn and gain access to the full version

Access to all documents
Unlimited textbook solutions
24/7 expert homework help

Lab 4 Selective Media & Agar BIO250L ” Data and Observa>ons In the table below, report the growth that was observed on each of the plates and their corresponding source. Denote whether there was growth on the nutrient plate and MSA plates, and then denote the color of the MSA around the colonies. Also denote any other perAnent observaAons. Table 2: MSA Growth Observa>ons Provide a clear, high resoluAon photo of your plates aWer incubaAon with the lids removed. For credit on this lab, your photo must show the growth and color that is reported in your tabulated data and must also include your handwriXen name in the background. Surface Tested Growth (Y/N) – Nutrient Agar Growth (Y/N) – MSA Agar MSA color around colonies (Red/Yellow) Other Observa>ons Skin No No No None Nose Yes No No cloudy has the most colonies Table Yes No No White, and cloudy Control No No No None

Lab 4 Selective Media & Agar BIO250L ”

Your preview ends here

Eager to read complete document? Join bartleby learn and gain access to the full version

Access to all documents
Unlimited textbook solutions
24/7 expert homework help

Lab 4 Selective Media & Agar BIO250L ” Skin/Nose MSA agar Surface/Control Nutrient Agar Surface/Control MSA Agar Skin/Nose Nutrient agar

Lab 4 Selective Media & Agar BIO250L ” Results and Discussion 1. Review the photo of your agar plates. Describe the color around the colonies. Did any of the colonies change the color of the MSA? Small dark 2. What does the color of the agar tell you about the bacteria taken from each area of your body? Color is very light EXPERIMENT 3: SELECTION AND DIFFERENTIATION OF GRAM-NEGATIVE BACTERIA Introductory Ques>ons 1. What are the objecAves of Experiment 3? What media will you use? How does this media pertain to the topic of selecAvity? Click here to ent 2. What results do you expect from Experiment 3 if your growth ferments lactose? Bacteria growth in different forms and size 3. What types of bacteria are inhibited on MacConkey agar? What ingredient(s) in MacConkey agar selects against those bacteria? Bile salts and crystal violet are selecAve agent, inhibit growth of gram-posiAve bacteria 4. What ingredient(s) makes MacConkey agar differenAal? Color and lactose 5. Look up the formula for MacConkey agar either in a microbiology textbook or online . Is this a chemically defined or a chemically complex media? Why is that important? chemically complex media because I can provide nutrients to the micro organisms Data and Observa>ons In the table below, report the growth that was observed on each of the plates. Also denote

Lab 4 Selective Media & Agar BIO250L ” Table 3: MacConkey Agar Results Provide a clear, high resoluAon photo of your plates aWer incubaAon with the lids removed. For credit on this lab, your photo must show the growth and color that is reported in your tabulated data and must also include your handwriXen name in the background. Sample Growth? (Yes or No) Colony Color (Clear or Red) Sink faucet Yes No Stream Yes Clear Toilet water Yes No

Your preview ends here

Eager to read complete document? Join bartleby learn and gain access to the full version

Access to all documents
Unlimited textbook solutions
24/7 expert homework help

Lab 4 Selective Media & Agar BIO250L ” Results and Discussion 1. Describe the growth you observed on the MacConkey Agar. Dark pink in color 2. For each plate, provide an analysis of the results. What does the color of the colonies for each of the samples mean regarding the interpretaAon of the results? Plate 1 Plate 2 Plate 3

Lab 4 Selective Media & Agar BIO250L ” Darker color

Related Documents

In-lab Ex15 Serial Dilutions Report.docx

intro to vet tech exam 6.docx

social impact of tech exam 4 and 5.docx

Screenshot 2024-01-06 at 5.44.39 PM.png

BIO201 Lab 6 pdff23.pdf

BIO250 Lab 2 micro pdf...pdf

BIO 101.docx

BIO202 Lab 18 Complete.docx

Recommended textbooks for you

Understanding Health Insurance: A Guide to Billin...

Health & Nutrition

ISBN:9781337679480

Author:GREEN

Publisher:Cengage

Principles Of Pharmacology Med Assist

Biology

ISBN:9781337512442

Author:RICE

Publisher:Cengage

3-2-1 Code It

Biology

ISBN:9781337660549

Author:GREEN

Publisher:Cengage

Essentials Health Info Management Principles/Prac...

Health & Nutrition

ISBN:9780357191651

Author:Bowie

Publisher:Cengage

Case Studies In Health Information Management

Biology

ISBN:9781337676908

Author:SCHNERING

Publisher:Cengage

Medical Terminology for Health Professions, Spira...

Health & Nutrition

ISBN:9781305634350

Author:Ann Ehrlich, Carol L. Schroeder, Laura Ehrlich, Katrina A. Schroeder

Publisher:Cengage Learning

SEE MORE TEXTBOOKS

Recommended textbooks for you

Understanding Health Insurance: A Guide to Billin...
Health & Nutrition
ISBN:9781337679480
Author:GREEN
Publisher:Cengage
Principles Of Pharmacology Med Assist
Biology
ISBN:9781337512442
Author:RICE
Publisher:Cengage
3-2-1 Code It
Biology
ISBN:9781337660549
Author:GREEN
Publisher:Cengage
Essentials Health Info Management Principles/Prac...
Health & Nutrition
ISBN:9780357191651
Author:Bowie
Publisher:Cengage
Case Studies In Health Information Management
Biology
ISBN:9781337676908
Author:SCHNERING
Publisher:Cengage
Medical Terminology for Health Professions, Spira...
Health & Nutrition
ISBN:9781305634350
Author:Ann Ehrlich, Carol L. Schroeder, Laura Ehrlich, Katrina A. Schroeder
Publisher:Cengage Learning