In-lab Ex15 Serial Dilutions Report

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Georgia State University *

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2310

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Biology

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Jan 9, 2024

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docx

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2

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BIOL 2310 In-Lab Ex. 15 Serial Dilution Name: Sara Fletcher Group #: 3 Lab Section: 034 1. What does CFU stand for and why is this term used instead of cells? CFU stands for colony forming unit. The term CFU is used instead of cells, because in some bacteria more than on cell may start a colony, or some visible cells may not be able to grow. 2. Why are some plates considered uncountable (TMTC or TFTC)? A countable plate is considered to have colonies between 30-300. Any plate that has too many to count (TMTC) or too few to count (TFTC) are considered uncountable. 3. After performing a viable plate count the following results were recorded. 10-5: 8632 colonies, 10-6: 854 colonies, 10-7: 87 colonies, 10-8: 7 colonies What was the original cell density for the bacterial culture tested? Original Cell Density: 8.7 x 10 9 CFU/mL Experimental : Perform the serial dilution as seen below (9.9ml tubes= yellow top, 9.0 ml= blue) starting with your 10 -4 dilution culture of Serratia marcescens. DF= 10 -4 DF= 10 -7 DF= 10 -8 What is the plated dilution factor for each of your two TSA plates? Plate 1 from the yellow top tube: 10 -6 Plate 2 from the blue top tube: 10 -7 RESULTS: Plated Dilution Factor Colony Count Countable Plate (Y/N) OCD Plate 1: 10 -6 854 colonies N 8.54 x 10 8 CFU/ ml 100µL 1 mL S. marcescens culture 9.0 mL H 2 O 9.9 mL H 2 O
Plate 2: 10 -7 87 colonies Y 8.7 X 10 9 CFU/mL
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