Microbiology and fermentation (lab exam 1) Flashcards _ Quizlet

3/25/24, 11:13 AM Microbiology and fermentation (lab exam 1) Flashcards | Quizlet https://quizlet.com/36381054/microbiology-and-fermentation-lab-exam-1-flash-cards/?funnelUUID=34fe10fe-6e62-4061-9971-3ccf01de499e 1/6 Microbiology and fermentation (lab exam 1) Leave the first rating Students also viewed Terms in this set (132) Others also viewed these textbooks Search for a textbook or question Hole's Human Anatomy and Physiology 13th Edition • ISBN: 9780073378275 (15 more) David N. Shier, Jackie L. Butler, Ricki Lewis 1,458 solutions Human Microbiome 61 terms ceabennett01 Preview Bio Test #2 74 terms MayoIzYum Preview BIOL 1720 Exam 3 35 terms amypritchard6712 Preview Microbiolog 9 terms Eric_Tand What is the maximum number of excused absences allowed? one week worth of class How many lab sessions can one miss before they are withdrawn? two consecutive lab sessions or a total of three Is lab mandatory Yes Can a student make up a lab if they miss their normal lab session yes as long as they attend a lab that is conducting the same lab work Is taping of lecture and lab permitted? NO If a culture is spilled should the student attempt to clean it up themselves? NO In the case of a culture spill, alert the instructor immediately!! The procedure for decontaminating a spill and the must be strictly followed!!! If misplaced items are found in your storage drawer, such as another students slide, where should you place them? In the "orphaned slides, tubes and plates" area Should one turn off the water faucets with bare clean hands after washing? No, always use a paper towel to turn off faucets! When cleaning up a spill how should you wipe down the bench? always use a rotating motion of the hand to bring the paper towels toward the middle of the spill area .... Never use the "windshield wiper" method!! The working distance on your microscope ... decreases with increasing magnification Microbiology and fermentation (lab exam 1) Study

3/25/24, 11:13 AM Microbiology and fermentation (lab exam 1) Flashcards | Quizlet https://quizlet.com/36381054/microbiology-and-fermentation-lab-exam-1-flash-cards/?funnelUUID=34fe10fe-6e62-4061-9971-3ccf01de499e 2/6 Why should you use only fine focus knob while viewing a specimen with the 100x objective? Because working distance is minimal and if the coarse adjustment is used you are likely to shatter the slide or the objective lens In case of accidental spill you would... remain at your work station until instructor comes to your assistance Why is a wet film of disinfectant left on the lab bench to air dry? If you wipe the lab bench completely dry there is no disinfectant left on the bench top which leaves the bench top open for contamination from the paper towel you're wiping with, aerosols and many other things To prevent aerosols from forming, wires that from loops and needles are flamed... from handle connection to tip (loop) Why are loops flame sterilized from handle to tip (loop) to prevent aerosols from forming and being spread When do you sterilize the inoculating loop? Before obtaining a sample and after transferring the bacteria to another medium or slide At a minimum how many times are you required to wash your hands for this lab? 2 (one at the start and one at the end) The iris diaphram of the microscope controls the amount of light passing through the condenser Which microscope is used to visualize viruses? Electron The total magnification of the specimen is 200x. If the ocular lens used on the microscope was 20x, then which objective lens was used? 10x Which staining technique results in an unstained cell against a dark or colored background? Negative staining The counterstain of the Gram stain is Safranin Iodine of the Gram stain serves as the mordant The basic categories of colony morphology are shape, margin, texture, elevation, pigment production A device used for counting colonies is called a colony counter a word that means in the absence of oxygen anaerobic The process for removing oxygen in the anaerobic jar works how? a packet containing chemiclas that react oxygen gas with hydrogen gas producing water a device used for producing an oxygen free environment is called an anaerobic jar How will you know when the anaerobic jar chamber is void of oxygen? the methylene blue indicator strip will appear clear or white Is the counter stain in an acid fast stain basic or acidic? basic Acid fast stains aid in the diagnosis of which diseases? leprosy, tuberculosis, and Nocardiosis The Gram and acid-fast stains are referred to as differential stains because They color various structures in bacterial cells contrasting colors The Schaeffer-Fulton staining procedure is used to stain cells with Endospores Microbiology and fermentation (lab exam 1)

3/25/24, 11:13 AM Microbiology and fermentation (lab exam 1) Flashcards | Quizlet https://quizlet.com/36381054/microbiology-and-fermentation-lab-exam-1-flash-cards/?funnelUUID=34fe10fe-6e62-4061-9971-3ccf01de499e 4/6 What type of stain would be used on cells that are too delicate to be heat fixed negative stain why do the cells look white in a negative stain? uses a dye (chromogen) solution in which the dye is acidic and carries a neg charge. The dye gives the cells a neg charge on the cell surface. The neg charge repels neg charged dye so the cells remain unstained. Is there a mordant or counterstain used in the negative stain no explain the acid fast staining procedure heat fix cells, stain with Carbolfuchsin (primary stain), decolorize (acid alcohol), counterstain with methylene blue or brilliant green, blot dry with bibulous paper what color are acid fast positive cells pink/ red regardless of counterstain used what color are acid fast neg cells blue/ green depending on which counter stain was used Explain how a capsule stain is done 1. Congo red (primary stain) this is acidic stain that stains the background 2. cells added to slide (not heat fixed) 3. Maneval's stain ( this is the basic stain that colorizes the cell) 4. blot with bibulous paper are cells heat fixed in an capsule stain no, they are applied into a drop of water or serum on the slide with Congo Red explain an endospore stain cells are heat fixed to a slide in an emulsion, 1. malachite green (primary stain) 2. safranin ( counter stain) in an endospore stain what color do the spores stain? the rest of the cell? spore- green rest of cell- pink/ red who was the father of Infection Control Ignaz Semmelweis what is resident microbiota flora of the skin that is present in an equilibrious state about how many resident microbiota are found to exist per square centimeter of the skin on hands and arms about 8 million microbial cells what is the process in which microbial cells split? binary fission do spirillium form chains? no what does the oil do in oil immersion microscopy it eliminates refractive rays and it essentially gathers all light rays and directs it into lens What does the iodine mordant do in gram staining it seals the crystal violet into the peptidolycan cell wall is there any stage in the gram staining process where a gram neg cell will be purple Yes at the begining when the crystal violet is added also when iodine is placed on slide, what catalyst is used in the anaerobic jar to remove any remaining oxygen palladium facultative anaerobe can grow in both aerobic and anaerobic conditions is E coli gram neg or gram pos Neg because its pink micro aerotolerant the microbe can tolerate small amounts of oxygen another name for the light microscope is bright field microscope Why is the price of using a stain on our cells staining usually kills the cells what is the practical limit of maginification of a light microscope 1300x Microbiology and fermentation (lab exam 1)

3/25/24, 11:13 AM Microbiology and fermentation (lab exam 1) Flashcards | Quizlet https://quizlet.com/36381054/microbiology-and-fermentation-lab-exam-1-flash-cards/?funnelUUID=34fe10fe-6e62-4061-9971-3ccf01de499e 5/6 The measurement of how far apart two points must be for the microscope to view them as being separate is called limit of resolution numerical aperture a measure of the lens ability to "capture " light coming from the specimen and use it to make an image resolution The ability of a lens to distinguish two points as separate and distinct at a specified distance apart is the ocular lens part of the primary lens system? yes and objective lens are secondary lenses parfocal lens that stays approximately in focus when the magnification is changed what are sphere shaped cells called coccus, cocci what are rod shaped cells called bacillus, bacilli what are spiral shaped cells called spirilla, spirillium what are flexible spiral shaped cells called spirochetes a cell shape of a slightly curved rods is called vibrios a cell that exhibits a variety of cell shapes is called pleomorphism tetrads and sarcina are only seen in what type of cell cocci when this becomes too small the cell usually divides to increase its surface area surface to volume ratio the portion of the chromogen that gives it its color is the chromophore what is a basic stain staining solution with a positively charged chromophore allowing them to be attracted to the neg charge on the cell surface thus staining the cell list several common basic stains methylene blue, crystal violet, safranin why do we heat fix cells to kill the bacteria to make them stick to the slide, and to coagulate cytoplasmic proteins to make them more visible and for better staining what is it called when you transfer something without contamination of the sample, the sterile medium, or the surroundings aseptic transfer/ technique where should media tubes be placed when not in use in a test tube rack why is an open broth tube held at an angle to minimize airborne contamination Microbiology and fermentation (lab exam 1)