Macromolecules_Result_Record (1)

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Feb 20, 2024

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Record Results for Macromolecules Lab Sample Monosaccharide (Benedict’s Test) Starch (IKI Test) Protein (Biuret Test) Lipid (Sudan IV Test) Positive Control *Glucose did not react successfully; a positive result would have shown a dark red color* Starch: Reacted strongly with the IKI reagent, became a bluish black. Egg White: This solution became a dark violet shade, showing that a protein-rich solution should display a strong purple hue Canola/Salad Oil: The red reagent separated to the top layer of the liquid, clearly displaying the top layer of lipids and a positive result. Negative Control Water: A light blue solution, transparent in appearance, shows a clear negative result. Water: Became slightly yellow but no darkening was seen. A solution without starch should remain light possibly with a yellow tint. Water: The water remained light blue, showing a solution with a lack of protein will not become darker or become purple. Water: The reagent remained distributed throughout the solution, displaying a clear negative result. Unknown 1 Unknown 1: Mild positive, bright green appearance. Unknown 1: Did not react, looks identical to the Unknown 1: Became a greyish color with no blue Unknown Solution 1 : (Theory is white vinegar) shows a

This shows there are more than traces of reducing sugars but there are not “moderate” results. negative control. or purple hues. Has been interpreted as negative and the color is believed to be because of the initial color of the unknown solution. negative result with the same distribution as the control negative test. This shows this unknown is not lipid heavy. Unknown 2 Unknown 2: Slightly opaque dark green, traces of reducing sugars present. Unknown 2: Became dark, almost purple color. Shows there is starch present but not as much as the positive control. Unknown 2: Became a dark purple color showing a positive result. Unknown 2: Negative as well, showing a consistent distribution of the reagent throughout the solution. Unknown 3 Unknown 3: Milky white and opaque, a small amount of blue present due to the reagent. Unknown 3: Did not react, remained light in color with no color shift other than the slight yellows shown in the other negative results. Unknown 3: Due to the milky color of the original solution, the reagent has turned the solution to a light purple color. This is interpreted as a positive result. Unknown 3 : Has become a light pink color, mixing the milky appearance of the unknown and the reagent has distributed evenly throughout the solution. Answer the following questions based on your results:

1. If any of the tests are positive for distilled water, what will be your conclusion? More than likely the water was contaminated, As the distilled water should contain none of the observed macromolecules there should be no observable reaction. 2. Diabetic patients are prescribed a low-carb diet. Based on your observations, how will you choose the food for a person with diabetes? I would recommend the use of Benedict’s test as it quickly will show if the object contains reducing sugars or foods that only had a negative or mild reaction to the IKI test. 3. A green leaf is partially covered with an opaque mask (black circle in Leaf A) for a week. During that week the region of the leaf under the mask receives no sunlight. The mask is removed and the leaf is stained using IKI. Color Leaf B to indicate the staining pattern seen in this leaf The area of the leaf under the mask would be stained a negative color. This is due to a lack of photosynthesis occurring under the mask and there being a lack of polysaccharides that would positively react.

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1: 0.740 2: 0.566 3: 0.325 4: 0.192 U: 0.5962

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