pH and Buffers Datasheet

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School

Clemson University *

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Course

1010

Subject

Biology

Date

Feb 20, 2024

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pdf

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2

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Biology 1101 Lab pH & Buffers Page 1 of 2 Bio 1101 Acids and Bases Lab Name:_________________ Lab Section & Time: Procedure 1. Litmus pH indicator What can you conclude about the pH tap water here at Clemson ? As you add acid, describe the color change. What is the current pH of the solution? If you were going to make a cabbage salad and you wanted the red cabbage to be bright red, what dressing could you use? (you may want to use information from the following section) Procedure 2, 3 and 4. pH of common solutions, pH meter and pH calculation Solution pH paper pH meter pH calculate vinegar household ammonia lemon juice cola black coffee household bleach nonfat milk HCl 100 mM HCl 10 mM HCl 1.0 mM Comment on the measured and calculated values for the HCl solutions. Table 2. pH of some common solutions measured by paper, meter and calculated
Biology 1101 Lab pH & Buffers Page 2 of 2 Procedure 5. Antacid effectiveness Product Number of drops of acid Rolaids Tums Maalox Generic Baking Soda Which antacid neutralizes the greatest amount of acid? Which one neutralizes the least? What can you conclude regarding the advertisements for these products? How did the baking soda behave in this test? Could you use baking soda instead of an OTC antacid? Procedure 6. Measuring the effect of a buffer on pH No. of drops of 0.1M HCl Pure Water Bicarbonate Sample 0 2 4 6 8 10 12 14 16 18 20 22 24 26 Table 3. Effectiveness of some common antacids (one dose dissolved in 100 ml water) measured by dropwise neutralization of 0.1ml HCL Table 4. pH change after acid addition to water and bicarbonate solution
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