photosynthesis_report_sheet 2

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BIO2420

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Feb 20, 2024

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PHOTOSYNTHESIS REPORT SHEET Download the report sheet, complete it, and then submit it via Blackboard email as an attachment. Do not change the format. All blue shaded areas require answers. I. Using the spectroscope Experiment (dry lab) II. Absorption spectrum Experiment (dry lab) Name: Daya Trevizo Date: View List colors not absorbed by the filter/extract (colors you see) Red filter Red Blue filter Blue Green filter Green Spinach extract Green, Yellow ,orange What is the relationship between the color of the filter and that filter’s absorption of light? Each filter absorbs most color that is not its own Both the spinach extract and the green filter are green in color. How does the spinach extract compare to the absorption spectrum of the green filter? The extract has some green yellow orange and red. 400 nm 425 450 475 500 525 550 575 600 625 650 675 700 Carrot extract 24.2 14.0 11.0 15.4 53.0 92.0 96.8 99.8 100.0 99.2 100.0 99.0 100.0 Spinach extract 20.2 11 20.6 43.8 77.6 80.6 85.4 79.4 73.6 74.6 46.4 66.8 93.0
Absorption Spectrum Graph for carrot extract ( orange line ) and spinach extract ( green line ), superimposed. (Fill table using Microsoft insert shapes or manually graph, scan, embed image into report.) What wavelengths and colors of light are not absorbed well (reflected/transmitted) by the carrot extract (carotenoids)? Yellow,orange.red
III. Pigment chromatography Experiment (dry lab) What wavelength and colors of light is not absorbed well (reflected/transmitted) by the spinach extract (chlorophylls mainly)? Green Which pigment has the greatest affinity for the solvent (is most soluble in the solvent and travels right along with the solvent)? Carotene Which pigment has the greatest affinity for the paper (is most attracted to the paper)? Chlorophyll b Distance traveled by the solvent in cm 54 mm = 5.4 cm Pigment color Distance traveled in cm (from baseline to pigment band) *Rf value (pigment distance divided by solvent distance) carotenes Orange 4.8 cm 4.8/5.4 = 0.89 xanthophylls Yellow 4.1cm 4.1/5.4=0.76 chlorophyll a Blue/green 2.5cm 2.5/5.4=0.46 chlorophyll b Yellow/green 1.5 cm 1.5/5.4=0.28
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IV. Starch localization Experiment (dry lab) V. Chloroplast structure What pigment do you think is present in the highest concentration during the spring season when many leaves of deciduous plants are green? Chlorophyll is responsible for the color. In the fall, leaves turn red, orange, yellow, and eventually brown. Where did the green color go? Break down of chlorophyll and carotenoids which make yellow and orange and anthocyanin which makes anthocyanin. What would be your hypothesis on the localization of starch? Center Does this correspond to the localization of chlorophyll pigments? Yes The ends of the leaf Which part of the leaf stores starch? (darker outer region or lighter inner region)
Experiment (dry lab) Match the descriptions to the labels below End of Lab ☺ Thylako id a. site for light reactions, electron transport chain and ATP-synthesizing enzymes Stroma b. Site for Calvin cycle Outer membr ane c. Surrounds the organelle Thylakoid Stroma Outer membrane

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