cellular_respiration_report_sheet 2-3

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CELLULAR RESPIRATION REPORT SHEET Download the report sheet, complete it, and then submit it via Blackboard email as an attachment. Do not change the format. All blue shaded areas require answers. I. Oxygen Consumption Experiment (dry lab) Plot a graph relating metabolic rate and mass. Name: Daya Trevizo Unit # 4 Organism type (common name) Mouse Organism mass (g) 22.5 g Trial No. Volume O 2 used (ml) Time interval (seconds) Time interval (hour) ml O 2 / g / hr 1 2ml 150 0.042 2.11 2 2ml 180 0.050 1.78 3 2ml 175 0.049 1.81 average 2ml 168.3 0.047 1.90 Animal Metabolic rate (ml O2/g/hr) Approximate mass Kangaroo mouse 1.8 0.02 kg Rat 0.87 1 kg Cat 0.68 2 kg Dog 0.33 7 kg Man 0.21 70 kg Elephant 0.07 1000 kg

(Fill table using Microsoft insert shapes or manually graph, scan, embed image into report.) II. CO 2 production in plant embryos Experiment (dry lab) As body mass increases, oxygen consumption ____. Decreases

Experiment (at home lab) III. The mitochondrion Experiment (dry lab) Match the descriptions to the labels below. seeds Phenol red color at 0 min (initial color) Phenol red color at 60 min (red, yellow, or fuchsia) Was there a change in pH? (yes or no) Is CO 2 present or absent? Bottle #1: (GERM) Red Yellow Yes Present Bottle #2: (GERM - Boil) Red Red No Absent Color of 5 ml of cabbage juice Purple Color of 5 ml of cabbage juice after blowing CO2 bubbles Lighter Purple seeds Cabbage juice color at 0 min Cabbage juice color at 60 min (lighter, darker or same color) Was there a change in pH? (yes or no) Is CO 2 present or absent? Jar #1: germinating ( boiled ) Violet Same No Absent Jar #2: germinating ( not boiled) Violet Lighter Yes Present 3 a. CO 2 production 2 b. Site for electron transport chain and ATP-synthesizing enzymes 4 c. Surrounds the organelle 1 d. Compartment between outer and inner membranes

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IV. Fermentation in yeast Experiment (at home lab) Record the amount of CO 2 (as none, some or most). Container # Amount of sucrose Estimate amount of CO 2 at 0 minutes Estimate amount of CO 2 at 12 hours (none, some or most) 1 1 teaspoon 0 Some 2 10 teaspoons 0 Most 3 0 0 None QUESTIONS: 1. We know that yeast utilizes glucose (monosaccharide) for energy and produces carbon dioxide. You added sucrose (disaccharide) to your yeast solution. Understanding the structure of sucrose, explain why your yeast solutions produce carbon dioxide? 4. Outer membrane 3. Matrix 2. Inner membrane/cristae 1. Intermembrane space

End of Lab ☺ Yeast can break down sucrose and fructose yeast also uses glucose for energy through the fermentation process 2. Which cup has the maximum CO 2 production? 1 3. Why? Higher sucrose concentration causing increased carbon dioxide.

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