Lab Report 2 Week 4

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Central Michigan University *

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215

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Biology

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Feb 20, 2024

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HSC 215 Wallace Lab Report Turn in your own college level work. Complete sentences, correct spelling, correct grammar and citations must be used. All work must be solely your own. Any plagiarism will result in a zero for the entire assignment. Labs must be submitted to lab instructors by the beginning of the lab period. Late labs will be accepted with a 50% reduction in the grade. Any lab reports submitted later than one week from the due date will not be awarded points. 1-3. (3 points) 1. (3 points) Convey your results and data relevant to the goals of the experiment by filling out the following table and preparing an additional alternate figure (bar graph, line graph etc.) to further depict the results of your experiment. Include titles for all tables and figures. DATA TIME Activity/ Intensity HEART RATE RPE 0 mins resting 84 BPM 6 1 2 3 4 Low Intensity 108 BPM 10 5 6 Low Intensity 108 BPM 10 7 Moderate to High 130 BPM 17 8 9 Recovery 10 110 BPM 13 11 12 110 BPM 10 13 14 4. (1 point) According to your heart rate data, did you achieve steady state exercise during the low intensity exercise? Did you experience EPOC? Explain your reasoning. No, my partner did not achieve steady-state exercise during the low-intensity exercise. She did experience EPOC with heavy breathing after the exercise. 5. (1 point) According to your heart rate data, did you achieve lactate threshold during the moderate to high intensity exercise? What proof do you have?
HSC 215 Wallace My partner achieved the lactate threshold in 10 minutes when she reached level 12 of exertion levels. %HRtarget= (200-84*.60) + = 153BPM 6-8. (3 points) A. Describe or hand draw the aerobic production of ATP including glycolysis, Krebs, and ETC. B. Include how many NADH, FADH2 and ATP are produced in each step. C. Attach the questions completed in class from Part 1-Small Group Recitation ATP Production to the back of your lab report. 1. Write the “big picture” formula for Aerobic metabolism of glucose. C6H12O6 (glucose) + 6 O2 (oxygen) → 6 CO2 (carbon dioxide) + 6 H2O (water) + Energy (in the form of ATP) 2. Provide the chemical formulas for glucose and pyruvate and lactate. Glucose: C6H1206 Pyruvate: C3H403 Lactate: C3H603 3. List the products produced during glycolysis if oxygen is present Pyruvate, 2 NADH (Nicotinamide Adenine Dinucleotide), 2 ATP (Adenosine Triphosphate) , and 2 NADH 4. List the products produced during glycolysis if oxygen is NOT present Lactic Acid, 2 ATP and 2 NADH molecules 5. Draw out the Krebs cycle, use your text or notes to include acetyl coA, oxaloacetic acid and citric acid. Indicate how many carbons each contain. Show how many ATP, NADH and FADH2 are produced during this step of energy production .
HSC 215 Wallace 6. Outline the steps of the ETC beginning with one NADH arriving from the mitochondrial matrix and ending with the electron being passed to the final electron acceptor and the production of water. ETC NADH and FADH2 arrive H is dropped off and an electron is removed – H+ Electrons are transferred to electron acceptors thus generating energy H+ is moved to intermembranous space H+ concertation increases H+ moves to matrix Channels contain ATP synthesis ADP+Pi
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HSC 215 Wallace 7. How many ATP are produced during the aerobic metabolism of one glucose molecule? 32-38 ATP 9. (1 point) Case study: Bernie is a 81 year old man who undergoes the same experiment we just performed. Calculate his max heart rate and his target heart rate and RPE to induce lactate threshold? His target heart rate should be approximately 92 BPM, and his maximum heart rate should be close to 107 BMP. When he reaches his lactate threshold, his RPE level should be between 6 and 8. 220-81=139 139-60*.4+60=92 target heart rate 220-81=139 139-60*.6+60=107 max heart rate 10. (1 point) Include correct and complete citations. Ferretti G;Fagoni N;Taboni A;Bruseghini P;Vinetti G; (n.d.).  The physiology of submaximal exercise: The Steady State Concept . Respiratory physiology & neurobiology. https://pubmed.ncbi.nlm.nih.gov/28818484/#:~:text=The%20steady%20state%20concept %20implies,physiological%20consequences%2C%20which%20are%20analysed.