Copy of Lab 2 Equipment Post-lab_Sp23

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Post-lab Assignment LAB 2: Equipment Use Bio 1AL Spring 2023 Last Name First Name Lab Section # Lab 2 Post-Lab 1) Based upon your absorption spectrum of DCPIP, record your value for λ max . (0.5 pt) λ max : 606.5nm 2) DCPIP standard curve a) Graph the quantitative relationship between absorbance and DCPIP concentration using the DCPIP standard curve absorbance values that you recorded. See the Correlation and Regression Analysis page in bCourses. Use an xy scatterplot chart type in Excel, and fit the data with a linear trendline. Show the equation of the trendline and R 2 value on the graph. Save the graph in Excel as an image ( Ctrl Click on the graph > Save as Picture or take a screenshot) and paste the image below. (1 pt) b) Describe in your own words what your R 2 value means. (0.5 pt) Our R^2 value means that 99.07% of the variation in our absorbance can be explained by the correlation to DCPIP concentration. Which means absorbance is strongly correlated to DCPIP concentration and the line of best fit is a good estimate. 2-1

Post-lab Assignment LAB 2: Equipment Use Bio 1AL Spring 2023 c) Use your standard curve to determine the concentration (in µM) of the unknown DCPIP solution. Report the absorbance that you measured for the unknown DCPIP solution. Use the equation of the trendline for your calculation, and show your work. Include units. (0.5 pt) Absorbance of unknown DCPIP solution: __.721________ Concentration of unknown DCPIP solution: ____38.8µM_____ (Show your work below). 0.721= 0.0182x-0.024 y= 38.3µM 3) Pipetting : Record your five values for pipetting 100 μL and those of your lab station partner. Note if you did not have a station partner then get the data from another student. If there were three students in your group, then please record the data of one of them. a) You will be running a t-test on your data. State the null hypothesis for the t-test. (0.5 pts) There is no difference between my lab partners data and my data for the observed weights of pipetting 100 μL. b) Calculate the mean, standard deviation (see Excel Graphing Resources), and percent error from the expected weight for the 100 μL sample. Run an Independent Sample t-test using VassarStats, and record the relevant values below. See the Comparing Two Means page in bCourses for guidance on performing and interpreting the t-test. (2 pts) Your data Lab partner’s data Observed weights (g) .1035 .1023 .1028 .1026 .1053 .1027 .1027 .1025 .1073 .1028 Mean weight (g) 0.1043 0.1026 Standard deviation (g) 0.0019652 0.00019235 Percent error from expected (%) 4.3% 2.65 t-test results t = __1.97_____, df = __4.08____ , p (2-tailed) = _0.120112________ c) Based on the t-test results, what should you do with regard to the null hypothesis? (circle one) (0.5 pt) Reject the null hypothesis Fail to reject the null hypothesis 2-2

Post-lab Assignment LAB 2: Equipment Use Bio 1AL Spring 2023 4) In lecture, micropipette accuracy and precision were discussed. (0.5 pt) Which statistical measurement is most relevant and likely to be altered by errors arising from problems with: Pipettor accuracy ? (Circle/Highlight one): Mean Standard Deviation Pipettor precision ? (Circle/Highlight one): Mean Standard Deviation 5) Reticle calibration . Complete the table below using the data you recorded in Table 3 of the Lab 2 Procedures. (1.5 pt) Total Magnification # of Subdivisions in 100 μm Calibration (μm) = distance between each subdivision 100X # = _10_____ 100 μm/# = ___10____ μm 200X # = _20_____ 100 μm/# = __5_____ μm 400X # = _40_____ 100 μm/# = __2.5_____ μm 6) Optical sections . As described in the Microscopy background reading and lecture, at high power objectives, the depth of field is very small. By repositioning the midpoint of the depth of field, you can generate different images (optical sections) of the specimen. Draw predicted images for each of the three focal points of the cell shown on the right side of Figure 8 of the Lab 2D - Using a Compound Microscope page in bCourses (where it says FP1, FP2 and FP3 in the picture). The images should correspond to what you would see using the 40x objective. The circles below represent the field of view. Remember you are looking down on the cell, while the image in the lab manual shows a side view. (0.5 pt) Label the location of the cilia, cell membrane, nucleus, and cytoplasm in your drawings (Note: not all structures can be observed at each given focal point.) You do not need to include a scale bar in these drawings because one is not provided in Figure 8. (0.5 pt) Drawing instructions: If you hand-drew the drawings on your Lab 2 Procedures or on a blank piece of paper, you can take a picture of the drawings and insert the image below. You may also double click on the image below and complete the drawings using the Google Drawing tools. 2-3

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Post-lab Assignment LAB 2: Equipment Use Bio 1AL Spring 2023 7) a) Depth of Field . Examine the slide with the solid glass rod and hollow glass tube. In the left circle, draw the appearance of a solid rod when the 20X objective is focused in the midpoint of the solid rod. In the right circle, draw the appearance of the hollow tube when focused in the midpoint of the hollow tube. Include scale bars labeled with the distance that they represent. (1 pt) Drawing instructions: If you hand-drew the drawings on your Lab 2 Procedures or on a blank piece of paper, you can take a picture of the drawings and insert the image below. You may also double click on the image below and complete the drawings using the Google Drawing tools. Solid Rod = 30 subdivisions across = 150 μm Hollow Tube = 40 subdivisions across = 200μm b) Determine which object is on top - the rod or the tube. (0.5 pt) Which is on top : the solid rod or the hollow tube? (Circle/highlight one) Rod Tube 2-4

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