Osmosis Workbook

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Coastal Carolina Community College *

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110

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Biology

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Feb 20, 2024

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docx

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Uploaded by MajorSummerKoala3

Osmosis TOTAL POINTS: 25 POINTS PRE-LAB QUESTIONS (2 POINTS) 1. Why don’t red blood cells swell or shrink in blood? a. Because the osmotic balance of blood plasma is isotonic to the blood cells cytoplasm. 2. What organelles or structures protect plant and animal cells from damage resulting from osmotic pressure? Explain what they are, how they work, and where they are located. a. Plant cells utilize a rigid cell wall made of cellulose to protect against osmotic pressure changes, preventing cell deformation. Animal cells depend on the cell membrane's selective transport mechanisms and contractile vacuoles to manage water content and maintain osmotic balance, ensuring cellular stability. EXPERIMENT 1: TONICITY AND THE ANIMAL CELL Data Tables (5 points) Table 1: Osmosis Results Solution Type Volume (mL) Before Osmosis Volume (mL) After Osmosis 100% Distilled Water 50 mL 30 mL 100% Corn Syrup 60 mL 79 mL 10% Corn Syrup/90% Distilled Water 50 mL 60 mL Control 0 mL 10 mL Table 2: Observations of Eggs After Osmosis © eScience Labs, 2018

Osmosis 100% Distilled Water 100% Corn Syrup 10% Corn Syrup/ 90% Distilled Water Control Soft Shriveled and Sticky Soft and a little sticky Looks the same Post-Lab Questions (6 points) 1. Write a hypothesis for each solution being tested. a. 100% water: Increase in size due to osmosis, water will move into the egg. b. 100% corn syrup: Hypertonic, decreases in size as water moves out of the egg to balance the high concentration of the corn syrup outside. c. 90/10 mix: Hypertonic, with water moving out of the egg, but might be less pronounced than in the 100% corn syrup solution due to the dilution with water. 2. How do each of the three eggs placed in solution compare to the control egg? a. The size of all the eggs changed. 3. For each beaker, identify whether the solution inside was hypotonic, hypertonic, or isotonic in comparison to the control beaker. a. 100% water: Isotonic b. 100% corn syrup: Hypertonic © eScience Labs, 2018

Osmosis c. 90/10 mix: Hypertonic 4. What was the direction of osmosis in the beaker labeled “100% distilled water”? Did the egg in this beaker burst? a. Moved into the egg. It grew but did not bust. 5. Was there any liquid in the “control” beaker after 24-48 hours? If so, why do you think this is? If not, why do you think this is? a. Yes, the water moved out of the egg to a less concentrated area. 6. Which solution contained a dehydrated cell? Think about someone with a high sugar diet. Hypothesize how their cells might be affected by osmosis. a. The 100% corn syrup. High sugar levels will expose cells to an osmotic imbalance, leading to dehydration, altered cellular function, and could possibly cause other metabolic and inflammatory disruptions due to the movement of water out of cells to balance concentrations of the outside to the inside cell environment. © eScience Labs, 2018

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Osmosis EXPERIMENT 2: TONICITY AND THE PLANT CELL Data Tables (5 points) Table 3: Water Displacement per Potato Sample Potato Type Potato Observations Sample Initial Displacement (mL) Final Displacement (mL) Net Displacement (mL) Sweet Orange, no skin A 6 mL 6 mL 0 mL Sweet Orange, no skin B 7 mL 4 mL -3 mL Honey Gold Light yellow, no skin A 5 mL 5 mL 0 mL Honey Gold Light yellow, no skin B 5 mL 3 mL -2 mL Post-Lab Questions (5 points) 1. How did the physical characteristics of the potato vary before and after the experiment? Did it vary by potato type? a. Before, the only difference was the type of potato being used. After, the slice that was in the NaCl was soft and somewhat bendable. Both types of potato had the same thing happen with the water and NaCl. 2. What does the net change in the potato sample indicate? a. More water entered the potato sample that was in actual water while the sample that was in the NaCl solution lost water. 3. Based on the data from this experiment, hypothesize which potato has the highest natural sugar concentration. Explain your reasoning. a. The sweet potato because its net displacement was higher. 4. Did water flow into or out of the plant cells (potato cells) in each of the samples examined? How do you know this? © eScience Labs, 2018

Osmosis a. The first sample had no displacement of water because the initial and final displacements were equal. The second sample, water flowed out of the potato with a net displacement of -3 mL. The third sample was the same as the first sample. The fourth sample had a net displacement of -2 mL, and the water flowed out of the potato. 5. Would this experiment work with other plant cells? What about with animal cells? Why or why not? a. Yes, because both plant and animal cells would shrink when placed in a hypertonic solution. PICTURE (2 POINTS): Insert a picture of yourself completing this lab to this word document prior to uploading for full credit. © eScience Labs, 2018

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