Lab Quiz 2

pdf

School

Arizona State University *

*We aren’t endorsed by this school

Course

206

Subject

Biology

Date

Feb 20, 2024

Type

pdf

Pages

Uploaded by SargentSeahorseMaster249

1. We used sodium dodecyl sulfate (SDS) in this lab. What is the purpose of using SDS? [3 points] The purpose of using SDS is because it denatures proteins and breaks up any polymers into their subunits. It also covers the protein with negative charges. 2. What is the purpose of using bromophenol blue dye in the SDS-PAGE sample? [2 points] Bromophenol blue is the tracking dye that makes it possible to see the protein as it is loaded into the gel, and indicates when the run is complete as it runs ahead of the protein bands. 3. The SDS-Polyacrylamide Gel Electrophoresis (SDS-PAGE) of a protein yields two bands corresponding to molecular weights of 15,000 Da and 35,000 Da. In a separate experiment, the total molecular weight of the native protein was determined to be 85,000 Da. Determine how many of each subunit are present in the native protein. [3 points] There would be two 35,000 Da and one 15,000 Da because 35,000 + 35,000 + 15,000 = 85,000 Da. 4. Explain why beta-mercaptoethanol (BME) is used in denaturing proteins. [2 points] BME is used to reduce the disulfide bonds so that it can irreversibly denature proteins. 5. Explain why SDS-PAGE gel needs to be rinsed in ultrapure water three times for five minutes each before the gel is stained in Coomassie Blue dye solution. [3 points]

6. The following molecular weight standard curve was obtained. Calculate Rm for each protein sample. Determine the molecular weight of the following protein samples. Which is a larger protein, A or B? [9 points] Protein Sample ID Distance protein band traveled in cm Distance tracking dye traveled in cm A 4.15 8.30 B 2.90 8.30 Protein A: Rm = 4.15/8.30 = 0.5 Y = -1.71x + 5.57 X (Rm) = 0.5 Y = -1.71 (0.5) + 5.57 Y = 4.715 Y = Log(MW)

MW = 10 Y = 10 4.715 = 51880 Protein B: Rm = 2.90/8.30 = 0.35 Y = -1.71x + 5.57 X (Rm) = 0.35 Y = -1.71 (0.35) + 5.57 Y = 4.9715 Y = Log(MW) MW = 10 Y = 10 4.9715 = 93648 7. You have a protein that is composed of five subunits. Two subunits are 19,000 Da each, two are 73,000 Da each, and the last one is 15,000 Da. How many bands will show up on SDS-PAGE gel? Sketch the protein bands on SDS-PAGE gel next to the molecular weight standards. [3 points]

Your preview ends here

Eager to read complete document? Join bartleby learn and gain access to the full version

Access to all documents
Unlimited textbook solutions
24/7 expert homework help

Related Documents

Chapter_013.docx

pierce_genetics7e_solutionsmanual_ch04.docx

pierce_genetics7e_solutionsmanual_ch05.docx

Recitation worksheet #3.docx

Micro exam 5.pdf

Physicology.pdf

Lab 3 Final, T6 update.pdf

Chap12 Exam Review.docx

Ch 2 Self-Paced Study Medterm.docx

Osmosis Workbook.docx

Intro to Science Workbook.docx

202301 lab 01 stats worksheet3.docx

Recommended textbooks for you

Aquaculture Science

Biology

ISBN:9781133558347

Author:Parker

Publisher:Cengage

Nutrition Now

Health & Nutrition

ISBN:9781337415408

Author:Brown

Publisher:Cengage

Biology (MindTap Course List)

Biology

ISBN:9781337392938

Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Publisher:Cengage Learning

Curren'S Math For Meds: Dosages & Sol

Nursing

ISBN:9781305143531

Author:CURREN

Publisher:Cengage

Biology 2e

Biology

ISBN:9781947172517

Author:Matthew Douglas, Jung Choi, Mary Ann Clark

Publisher:OpenStax

Biology: The Dynamic Science (MindTap Course List)

Biology

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Cengage Learning

SEE MORE TEXTBOOKS

Recommended textbooks for you

Aquaculture Science
Biology
ISBN:9781133558347
Author:Parker
Publisher:Cengage
Nutrition Now
Health & Nutrition
ISBN:9781337415408
Author:Brown
Publisher:Cengage
Biology (MindTap Course List)
Biology
ISBN:9781337392938
Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg
Publisher:Cengage Learning
Curren'S Math For Meds: Dosages & Sol
Nursing
ISBN:9781305143531
Author:CURREN
Publisher:Cengage
Biology 2e
Biology
ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:OpenStax
Biology: The Dynamic Science (MindTap Course List)
Biology
ISBN:9781305389892
Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher:Cengage Learning