Lab week 3

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Chamberlain University College of Nursing *

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BIOS251

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Biology

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Feb 20, 2024

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pdf

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BIOS251 OL, Week 3 Lab Name: LaShaye Gardner OL Lab 3: Cell Membrane and Transport: Learn how transporters keep cells healthy Learning Objectives: • Describe the plasma membrane structure using the fluid mosaic model • Recognize the relative permeability of lipid bilayers to different classes of molecule • Compare active and passive transport of molecules • Identify the 3 modes of active transport and the different classes of ion channel and carrier molecules • Relate the expression of specific transport proteins to the cell’s role Introduction : A variety of substances move in and out of the cells through the plasma membrane. Membrane transport is vital for cellular activities. The cells need to be healthy for the body to work efficiently. From filtration in the kidneys to the contraction of muscles during exercise, membrane transport contributes to many processes. In this simulation, you will learn about the structure and function of the cell membrane, and discover why membrane transporters are vital for healthy cells and the function of organ systems. In the first part of your mission, you will be introduced to the concepts of selective permeability and the fluid mosaic models of the plasma membrane. You will explore why cells need specialized transporter proteins to transport cargo molecules across their membranes. While some molecules can diffuse across the cell membrane, most molecules require a transporter protein to enter or leave the cell. In the later part of the simulation, you will set up a fluorescence microscopy experiment to learn how the channels, carriers, and pumps that exist in the membrane ensures that only the right molecules enter under the right conditions and help not only to keep the cell healthy but also help organ systems to function. Part 1: Complete Labster Cell Membrane and Transport
BIOS251 OL, Week 3 Lab Name: LaShaye Gardner Part 2: Report and Reflection Purpose: Describe in your own words and in complete sentences, the purpose of this experiment. The purpose of this experiment was to see how different molecules move across the cell membrane. We were able to observe special transport proteins in action.We were also able to determine why certain molecules need transport and other need diffusion. Observations: List 2 observations you have made in this simulation. Observation 1: My first observation was that all of the molecules couldn’t get through certain parts of the cell. This furthered my insight on transport and diffusion. Observation 2: When viewed through microscope I didn’t understand the use of the florescent colors. please explain this to me. Answer all the questions below: 1. In complete sentences and in your own words define the following cellular transport: (2 points) a. Diffusion: Diffusion is a form of passive transport where molecules go from a high concentration to a low concentration. Diffusion doesn’t require energy from the cell. Diffusion makes molecules on both sides of the cell membrane equal. b. Osmosis: Osmosis is another example of passive transport. Osmosis is when water molecules move across the cell membrane. Water moves from a lower solute concentration to a higher solute concentration. Osmosis goal is to maintain balance and equalize each side. c. Facilitated diffusion: Facilitated diffusion is also a form of passive transports that uses special proteins to move molecules across the cell membrane. These proteins act as channel proteins or carrier proteins allowing specific molecules to lower concentration gradient.
BIOS251 OL, Week 3 Lab Name: LaShaye Gardner d. Active transport: Active transport is a for of cellular transport that relies on the energy from ATP. Active transport moves substances against their concentration to a higher concentration. 2. Using the figure below, identify the cellular structures and its function.(6 points) Cell Structure Functions A nucleus Control center for cell B EPR Store and move things made by the cell
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BIOS251 OL, Week 3 Lab Name: LaShaye Gardner C DNA hold coded information D cytoplasm hold cells organelles E golgi stores and transports protein F cell membrane allow for in and out passage to cell G mitochondria powerhouse, produces energy H ribosome chemical messenger that runs cell I lysosome digests cell nutrients
BIOS251 OL, Week 3 Lab Name: LaShaye Gardner 3. What is tonicity and how does it affect the condition of the cell? (1 point) Tonicity is the ability of a solution to affect the fluid volume and pressure in a cell. A hypotonic solution has a low concentration of non permeating solutes than ICF. Hypotonic cells may swell due to absorption of water, this can cause the cell to burst. Hypertonic solutions have higher concentration of non permeating solutes than ICF. This causes a cell to shrivel due to loss of water. Isotonic solutions have the total concentration of non permeating solutes and are the same ICF. Isotonic solutions cause no change in the cells shape nor the cells volume. 4. When is a hypotonic IV solution administered to patients? Why? (1 point) A hypotonic IV solution is administered when the body is in need of fluids. This causes osmosis and the cell is able to hydrate itself. Water molecules flow from outside the cell to the inside cause the cell to expand. Hypotonic solutions are administered in times of dehydration. 5. Reflection: Reflect on at least 2 key concepts you have learned from this simulation. How would you relate it to the physiological functions of the body? The first major concept I learned was taking things for one concentration to another. This was necessary for me to visualize this process to gain a deeper knowledge of it. another concept I was able to take from this simulation was better understanding on how transport and diffusion is happening everyday and how this also ties in to homeostasis. Grading Rubric:
BIOS251 OL, Week 3 Lab Name: LaShaye Gardner Activity Deliverable Points Part 1 Complete simulation 15 Part 2 Complete lab report and answer questions • Purpose (1 point) • Observation (2 points) • Questions (10 points) • Reflection (2 points) 15 Total Complete all lab activities 30
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