The reason for atomic emission to be more sensitive to flame instability than atomic absorption or fluorescence needs to be explained. Concept introduction: Spectral lines help in identifying the atoms and molecules. These are a result of absorption or emission of light in a comparatively narrower frequency range as compared to the frequencies nearby. Fluorescence is the process by which a substance absorbing electromagnetic radiation emits light. In a defined space, the amount of a substance gives its concentration. A capacity of substance to absorb specific wavelength light gives its absorbance.

Question

(a 4 shows scanning electron microscope (SEM) images of extruded actions of packing bed for two capillary columns of different diameters, al 750 (bottom image) and b) 30-μm-i.d. Both columns are packed with the same stationary phase, spherical particles with 1-um diameter. A) When the columns were prepared, the figure shows that the column with the larger diameter has more packing irregularities. Explain this observation. B) Predict what affect this should have on band broadening and discuss your prediction using the van Deemter terms. C) Does this figure support your explanations in application question 33? Explain why or why not and make any changes in your answers in light of this figure. Figure 4 SEM images of sections of packed columns for a) 750 and b) 30-um-i.d. capillary columns.³

Answer 1

Question

Chapter 9, Problem 9.9QAP

Interpretation Introduction

Interpretation:

The reason for atomic emission to be more sensitive to flame instability than atomic absorption or fluorescence needs to be explained.

Concept introduction:

Spectral lines help in identifying the atoms and molecules. These are a result of absorption or emission of light in a comparatively narrower frequency range as compared to the frequencies nearby.

Fluorescence is the process by which a substance absorbing electromagnetic radiation emits light.

In a defined space, the amount of a substance gives its concentration. A capacity of substance to absorb specific wavelength light gives its absorbance.

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(a 4 shows scanning electron microscope (SEM) images of extruded actions of packing bed for two capillary columns of different diameters, al 750 (bottom image) and b) 30-μm-i.d. Both columns are packed with the same stationary phase, spherical particles with 1-um diameter. A) When the columns were prepared, the figure shows that the column with the larger diameter has more packing irregularities. Explain this observation. B) Predict what affect this should have on band broadening and discuss your prediction using the van Deemter terms. C) Does this figure support your explanations in application question 33? Explain why or why not and make any changes in your answers in light of this figure. Figure 4 SEM images of sections of packed columns for a) 750 and b) 30-um-i.d. capillary columns.³

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