MICROBIOLOGY: EVOLV.SCI.-W/ACCESS>CI<
MICROBIOLOGY: EVOLV.SCI.-W/ACCESS>CI<
4th Edition
ISBN: 9780393622805
Author: SLONCZEWSKI
Publisher: Norton custom
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Chapter 9, Problem 3RQ
Summary Introduction

To review:

The term competence and its role in the population.

Introduction:

Gene transfer is the method by which the foreign gene is inserted into the cell. There are two methods of gene transfer: horizontal gene transfer and vertical gene transfer. Transformation can occur naturally or the genes can be artificially introduced into the host to modify its genome. A special protein complex called transformasome is required in natural gene transfer.

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You aim to test the hypothesis that the Tbx4 and Tbx5 genes inhibit each other's expression during limb development. With access to chicken embryos and viruses capable of overexpressing Tbx4 and Tbx5, describe an experiment to investigate whether these genes suppress each other's expression in the limb buds. What results would you expect if they do repress each other? What results would you expect if they do not repress each other?
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You implant an FGF10-coated bead into the anterior flank of a chicken embryo, directly below the level of the wing bud. What is the phenotype of the resulting ectopic limb?  Briefly describe the expected expression domains of 1) Shh, 2) Tbx4, and 3) Tbx5 in the resulting ectopic limb bud.
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QCE Biology: Introduction to Gene Expression; Author: Atomi;https://www.youtube.com/watch?v=a7hydUtCIJk;License: Standard YouTube License, CC-BY