Genetics: From Genes To Genomes (6th International Edition)
6th Edition
ISBN: 9781260041217
Author: Leland Hartwell Dr., ? Michael L. Goldberg Professor Dr., ? Janice Fischer, ? Leroy Hood Dr.
Publisher: Mcgraw-Hill
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Chapter 9, Problem 15P
A recombinant DNA molecule is constructed using a plasmid vector called pMBG36 that is 4271 bp long. The pMBG36 plasmid contains a so-called polylinker that has a single site for each of several restriction enzymes, including BamHI (5′ G^GATCC 3′) and EcoRI (5′ G^AATTC 3′). The sequence of the polylinker region of pMBG36 is shown below; the dots indicate the large majority of the vector that is not shown. You now cut
pMBG36 with EcoRI and insert into it a fragment of the DNA previously shown in Problem 4 that is also cut with EcoRI.
| a. | Write out as much of the DNA sequence of the resultant recombinant DNA molecule as is possible. Two answers are possible; you need to show only one. |
| b. | Why are there two possible answers to part (a)? |
| c. | How many recognition sites for BamHI will be found in the recombinant DNA molecule shown in your answer to part (a)? |
| d. | If you cut this recombinant DNA molecule with BamHI and run the digest on a gel, how many bands would you see and how large would they be? |
| e. | How many recognition sites for EcoRI will be found in the recombinant DNA molecule shown in your answer to part (a)? |
| f. | If you cut this recombinant DNA molecule with EcoRI and run the digest on a gel, how many bands would you see and how large would they be? |
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Chapter 9 Solutions
Genetics: From Genes To Genomes (6th International Edition)
Ch. 9 - Match each of the terms in the left column to the...Ch. 9 - For each of the restriction enzymes listed below:...Ch. 9 - The calculations of the average restriction...Ch. 9 - The DNA molecule whose entire sequence follows is...Ch. 9 - Why do longer DNA molecules move more slowly than...Ch. 9 - Agarose gels with different average pore sizes are...Ch. 9 - The following picture shows the ethidium...Ch. 9 - The linear bacteriophage genomic DNA has at each...Ch. 9 - Consider a partial restriction digestion, in which...Ch. 9 - The text stated that molecular biologists have...
Ch. 9 - a. What is the purpose of molecular cloning? b....Ch. 9 - a. DNA polymerase b. RNA polymerase c. A...Ch. 9 - Is it possible that two different restriction...Ch. 9 - A plasmid vector pBS281 is cleaved by the enzyme...Ch. 9 - A recombinant DNA molecule is constructed using a...Ch. 9 - Suppose you are using a plasmid cloning vector...Ch. 9 - Prob. 17PCh. 9 - The lacZ gene from E. coli encodes the enzyme...Ch. 9 - Your undergraduate research advisor has assigned...Ch. 9 - Which of the enzymes from the following list would...Ch. 9 - You use the primer 5 GCCTCGAATCGGGTACC 3 to...Ch. 9 - a. To make a genomic library useful for sequencing...Ch. 9 - Problem 15 showed part of the sequence of the...Ch. 9 - Eukaryotic genomes are replete with repetitive...
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