Genetics: From Genes To Genomes (6th International Edition)
6th Edition
ISBN: 9781260041217
Author: Leland Hartwell Dr., ? Michael L. Goldberg Professor Dr., ? Janice Fischer, ? Leroy Hood Dr.
Publisher: Mcgraw-Hill
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Textbook Question
Chapter 9, Problem 9P
Consider a partial restriction digestion, in which genomic DNA is exposed to a small, limiting amount of a restriction enzyme for a very short period of time.
| a. | Would the resultant fragments be longer or shorter or the same size as those produced by a complete digestion? |
| b. | If you prepared genomic DNA from a tissue sample containing millions of cells, would the fragments produced by partial digestion of DNA from all of these cells be the same or different? |
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The shape of radishes may be long (SL/SL), oval (SL/SS), or round (SS/SS), and the color of radishes may be red (CR/CR), purple (CR/CW) or white (CW/CW). If a long, red radish plant is crossed with a round, white plant, what will be the appearance of the F1 and F2 generations?
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Question #3:
In the KeyGene paper, the authors state that it would be useful if pollen from an apomict would
transmit apomixis-inducing genes to the female in the cross (assuming the pollen is viable). Assuming
there was just one gene conferring gametophytic obligate apomixis, and that the two parents are
inbreds, what would be the consequences of such a cross if:
a) The apomixis was a dominant trait? Indicate the genotypes and phenotypes (apomict or non-
apomict) of the parents, F1 and F2 generations. Remember to include the expected genotypic
and phenotypic ratios (or percentages) in the F1 and F2 generations, and to position the female
first (left side) in the parental cross.
b) The apomixis was a recessive trait? Indicate the genotypes and phenotypes (apomict or non-
apomict) of the parents, F1 and F2 generations. Remember to include the expected genotypic
and phenotypic ratios (or percentages) in the F1 and F2 generations, and to position the female
first (left side) in the…
Chapter 9 Solutions
Genetics: From Genes To Genomes (6th International Edition)
Ch. 9 - Match each of the terms in the left column to the...Ch. 9 - For each of the restriction enzymes listed below:...Ch. 9 - The calculations of the average restriction...Ch. 9 - The DNA molecule whose entire sequence follows is...Ch. 9 - Why do longer DNA molecules move more slowly than...Ch. 9 - Agarose gels with different average pore sizes are...Ch. 9 - The following picture shows the ethidium...Ch. 9 - The linear bacteriophage genomic DNA has at each...Ch. 9 - Consider a partial restriction digestion, in which...Ch. 9 - The text stated that molecular biologists have...
Ch. 9 - a. What is the purpose of molecular cloning? b....Ch. 9 - a. DNA polymerase b. RNA polymerase c. A...Ch. 9 - Is it possible that two different restriction...Ch. 9 - A plasmid vector pBS281 is cleaved by the enzyme...Ch. 9 - A recombinant DNA molecule is constructed using a...Ch. 9 - Suppose you are using a plasmid cloning vector...Ch. 9 - Prob. 17PCh. 9 - The lacZ gene from E. coli encodes the enzyme...Ch. 9 - Your undergraduate research advisor has assigned...Ch. 9 - Which of the enzymes from the following list would...Ch. 9 - You use the primer 5 GCCTCGAATCGGGTACC 3 to...Ch. 9 - a. To make a genomic library useful for sequencing...Ch. 9 - Problem 15 showed part of the sequence of the...Ch. 9 - Eukaryotic genomes are replete with repetitive...
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