Chapter 9, Problem 2P

For each of the restriction enzymes listed below: (i) Approximately how many restriction fragments would result from digestion of the human genome (3 × 10⁹ bases) with the enzyme? (ii) Estimate the average size of the pieces of the human genome produced by digestion with the enzyme. (iii) State whether the fragments of human DNA produced by digestion with the given restriction enzyme would have sticky ends with a 5′ overhang, sticky ends with a 3′ overhang, or blunt ends. (iv) If the enzyme produces sticky ends, would all the overhangs on all the ends produced on all fragments of the human genome with that enzyme be identical, or not? (The recognition sequence on one strand for each enzyme is given in parentheses, with the 5′ end written at the left. N means any of the four nucleotides; R is any purine—that is, A or G; and Y is any pyrimidine—that is, C or T. ^ marks the site of cleavage.)

a.	Sau3A (^GATC)
b.	BamHI (G^GATCC)
c.	HpaII (C^CGG)
d.	SphI (GCATG^C)
e.	NaeI (GCC^GGC)
f.	BanI (G^GYRCC)
g.	BstYI (R^GATCY)
h.	BslI (CCNNNNN^NNGG)
i.	SbfI (CCTGCA^GG)