About the feedback inhibition and the mechanism by which the figure 1 fits into the systems diagram, shown in figure 2. Given: The feedback inhibition mechanism of a metabolic pathway is represented in the form of a diagram as follows: The systems diagram representing different pathways and their intersections is represented in figure 2. A metabolic pathway catalyzed by an enzyme is represented by each line. Each node represents a metabolite (small molecule). Several metabolic pathways and metabolites intersect and overlap each other. Figure 2: A systems diagram representing complex interactions of several metabolic pathways, their points or intersection and overlap, which takes place inside the human body. Introduction: A feedback is the mechanism utilized by the end product of a reaction sends feedback about the continuity of reaction. It is the mechanism by which hormone in the blood/body is maintained. The mechanism can be positive or negative. The positive feedback mechanism consists of cycles when end product of a reaction sends feedback about continuity of reaction. For example, release of oxytocin hormone during childbirth. The negative feedback mechanism consists of cycles when end product of a reaction sends feedback about inhibition of reaction. For example, in case of insulin, hormone is used in regulation of glucose.

Question

You aim to test the hypothesis that the Tbx4 and Tbx5 genes inhibit each other's expression during limb development. With access to chicken embryos and viruses capable of overexpressing Tbx4 and Tbx5, describe an experiment to investigate whether these genes suppress each other's expression in the limb buds. What results would you expect if they do repress each other? What results would you expect if they do not repress each other?

Answer 1

Question

Chapter 8.5, Problem 1R

Summary Introduction

To review:

About the feedback inhibition and the mechanism by which the figure 1 fits into the systems diagram, shown in figure 2.

Given:

The feedback inhibition mechanism of a metabolic pathway is represented in the form of a diagram as follows:

The systems diagram representing different pathways and their intersections is represented in figure 2.

A metabolic pathway catalyzed by an enzyme is represented by each line.

Each node represents a metabolite (small molecule).

Several metabolic pathways and metabolites intersect and overlap each other.

Figure 2: A systems diagram representing complex interactions of several metabolic pathways, their points or intersection and overlap, which takes place inside the human body.

Introduction:

A feedback is the mechanism utilized by the end product of a reaction sends feedback about the continuity of reaction. It is the mechanism by which hormone in the blood/body is maintained. The mechanism can be positive or negative. The positive feedback mechanism consists of cycles when end product of a reaction sends feedback about continuity of reaction. For example, release of oxytocin hormone during childbirth. The negative feedback mechanism consists of cycles when end product of a reaction sends feedback about inhibition of reaction. For example, in case of insulin, hormone is used in regulation of glucose.

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