EP MICROBIOLOGY:W/DISEASES BY..-MOD.ACC
5th Edition
ISBN: 9780134607894
Author: BAUMAN
Publisher: PEARSON CO
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Chapter 8, Problem 8MC
Summary Introduction
Introduction:
The recombinant DNA technology involves isolation of genetic materials (DNA) from donor organisms (human, plant, or microorganism) and inserts them to recipient organisms after genetic manipulation. The main tools involved in the techniques are
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____ cut(s) DNA molecules at specific sites.
a.
DNA probes
b.
Restriction enzymes
c.
DNA polymerase
d.
DNA ligase
Features of restriction enzymes include the following, except __________ .
a. They are produced by both prokaryotes and eukaryotesb. may be type IIc. make single and double stranded breaks in DNAd. operate at a single temperaturee. Are used in nature to destroy bacteriophage DNA
_____________ are sequences of identical DNA when it's read from the 5' to 3' direction on one strand and the 5' to 3' direction on the other strand.
a. Palindromes
b. snRNPs
c. RFLPs
d. Restriction fragments
e. Restriction endonucleases
Chapter 8 Solutions
EP MICROBIOLOGY:W/DISEASES BY..-MOD.ACC
Ch. 8 - Why arent the terms recombinant DNA technology...Ch. 8 - Prob. 2TMWCh. 8 - Why wasnt polymerase chain reaction (PCR)...Ch. 8 - Why dont doctors routinely insert genes into their...Ch. 8 - Prob. 5TMWCh. 8 - Which of the following statements is true...Ch. 8 - A DNA gene synthesized from an RNA template is...Ch. 8 - Prob. 3MCCh. 8 - Prob. 4MCCh. 8 - Prob. 5MC
Ch. 8 - Prob. 6MCCh. 8 - Prob. 7MCCh. 8 - Prob. 8MCCh. 8 - Prob. 9MCCh. 8 - Prob. 10MCCh. 8 - Modified True/False 1. ________ Restriction...Ch. 8 - Modified True/False 2. ________ Restriction...Ch. 8 - Prob. 3MTFCh. 8 - Prob. 4MTFCh. 8 - Prob. 5MTFCh. 8 - Label the reagents and steps of PCR on the figure...Ch. 8 - Describe three artificial methods of introducing...Ch. 8 - Prob. 2SACh. 8 - Prob. 3SACh. 8 - Prob. 4SACh. 8 - List three potential problems of recombinant DNA...Ch. 8 - Examine the restriction sites listed in Table 8.1...Ch. 8 - CRITICAL THINKING 2 A cancer-inducing virus,...Ch. 8 - A thermocycler uses DNA polymerase from...Ch. 8 - Prob. 4CTCh. 8 - Prob. 5CTCh. 8 - Prob. 6CTCh. 8 - Prob. 7CTCh. 8 - Prob. 8CTCh. 8 - Prob. 9CTCh. 8 - Using the following terms, fill in the following...
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- Match the method with the appropriate enzyme.___ PCR a. Taq polymerase___ cutting DNA b. DNA ligase___ cDNA synthesis c. reverse transcriptase___ DNA sequencing d. restriction enzyme___ pasting DNA e. DNA polymerase (not Taq)arrow_forwardDNA can be separated based on fragment size using ________. a. restriction enzymes b. gel electrophoresis c. PCRarrow_forwardA DNA probe with sequence TCAGGCTTCAG would bind most strongly to which of the following DNA fragments? a. AGTCCGAAGTC c. GACTTCGGACT b. TCAGGCTTCAG d. UGAGGCUUGAGarrow_forward
- A DNA fingerprint is based on _____. a repeating sequences found on noncoding regions b the presence of restriction enzymes c a single repeating region d repeating sequences found on coding regionsarrow_forwardRNA can be used as a template for the production of DNA through the action of ____. a. ligase b. DNA polymerase c. reverse transcriptase d. restriction endonuclease e. RNA polymerasearrow_forwardDefinition of Terms( This is all about Applications of Recombinant DNA){ 2-3 sentences only) a. Clone b. Plasmids c. Biotechnology a. PCR Amplification b. Detection c. Modified Trait d. Human Genome e. Genetic Modified Organismarrow_forward
- Enzymes used to cut genes in recombinant DNA research are known as ____. a. DNA polymerases b. transcriptases c. replicases d. ligases e. restriction enzymesarrow_forwardcatalyzes the cleavage of DNA at a specific ii fragments. The statement given above is completed nucleotide sequence. splices DNA into by the information in row Row i ii A. DNA polymerase DNA ligase B. restriction DNA ligase endonuclease DNA Ligase restriction endonuclease restriction endonuclease С. D. restriction endonuclease A OB C Darrow_forwardin Cohen-Boyer's recombinant DNA procedure _______ must be used for both the bacterial DNA and the amphibian DNA _______. a. the same restrictions enzyme, so that the the restriction site are identical in the DNA of each species b. different restriction enzymes, so that the genes outside the restriction site are maintained c. the same restriction enzymes, to ensure that the newly formed DNA can replicate d. different restriction enzymes, to ensure that the newly introducted genes are maintained in the bacterial DNAarrow_forward
- Please explain right answerarrow_forwardWhich DNA polymerase in prokaryotes is involved in the replacement of primers with deoxynucleotides? a.DNA-Pol I b.DNA-Pol II c.DNA-Pol Ill d.DNA-Pol IVarrow_forwardRFLP analysis involves the following, except ________ . a. more than one restriction sites b.gene therapy c.intercalation of dye d.electrophoresis e.restrictionarrow_forward
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