EP MICROBIOLOGY:W/DISEASES BY..-MOD.ACC
EP MICROBIOLOGY:W/DISEASES BY..-MOD.ACC
5th Edition
ISBN: 9780134607894
Author: BAUMAN
Publisher: PEARSON CO
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Chapter 8, Problem 3MC
Summary Introduction

Introduction:

Change in the DNA sequence or structure of the genome is termed as mutation. Errors during replication and environmental factors can cause mutation. Usually DNA repair mechanism corrects the changes in the nucleotide sequence, however, uncorrected changes leads to mutation. The mutation inherits from one generation to the next generation and also, it actively involved in the evolutionary changes.

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From your previous experiment, you found that this enhancer activates stripe 2 of eve expression. When you sequence this enhancer you find several binding sites for the gap gene, Giant. To test how Giant interacts with eve, you decide to remove all of the Giant binding sites from the eve enhancer. What results do you expect to see with respect to eve expression?
What experiment could you do to see if the maternal gene, bicoid, is sufficient to form anterior fates?
You’re curious about the effect that gap genes have on the pair-rule gene, evenskipped (eve), so you isolate and sequence each of the eve enhancers. You’re particularly interested in one of the enhancers, which is just upstream of the eve gene.  Describe an experimental technique you would use to find out where this particular eve enhancer is active.
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