Chapter 8, Problem 8.69P

Interpretation Introduction

Interpretation:

An equation for the reaction of acidic form of TRIS with NaOH should be explained.

Concept Introduction:

The acidic form of TRIS is written as TRIS- ${\text{H}}^{+}$ or ${\left({\text{HOCH}}_2\right)}_2{\text{CNH}}_3^{+}$. This is buffer that consists of zwitter ions molecules which have both positive and negative charges. They are concentration and temperature change resistant. To understand this buffer it is not important to know their structure only the $\text{p}{K}_{\text{a}}$ values of the buffer and the needed concentration are enough to calculate its pH using Handerson-Hasselbalch equation.