Chapter 8, Problem 50P

A mutant B. adonis bacterium has a nonsense suppressor tRNA that inserts glutamine (Gln) to match UAG (bu tnot other nonsense) codons. [This species does not modify wobble position C residues to k C and does not havetRNA^Pyl (see Problem 57).]

a.	What is the anticodon of the suppressing tRNA? Indicate the 5′ and 3′ ends.
b.	What is the sequence of the template strand of the wild-type tRNAGln encoding gene that was altered to produce the suppressor, assuming that only a single-base-pair alteration was involved?
c.	What is the minimum number of tRNAGln genes that could be present in a wild-type B. adonis cell? Describe the corresponding anticodons.