Chapter 8, Problem 3P

Summary Introduction

To examine: Whether the statement, “If each cycle of PCR doubles the amount of DNA synthesized in the previous cycle, then 10 cycles will give a 10³-fold amplification, 20 cycles will give a 10⁶-fold amplification, and 30 cycles will give a 10⁹-fold amplification.” is true or false.

Concept introduction:

Polymerase chain reaction (PCR) is a molecular biology technique that operates to amplify a single copy or few copies of a DNA segment through various orders of magnitude. That generates millions of particular DNA sequence copies. PCR technique is used in research laboratory for various applications. These involve DNA cloning for sequencing, gene manipulation, gene cloning, functional analysis of genes, amplification of ancient DNA, analysis of genetic fingerprints for DNA profiling.