EBK BIOLOGY
5th Edition
ISBN: 8220101337627
Author: Maier
Publisher: PEARSON
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Question
Chapter 8, Problem 2AAATB
Summary Introduction
To write:
The DNA profile of two identical twin sisters and their biological parents.
Introduction:
DNA profile is a very commonly used technique to detect the paternity testing. The child gets some chromosomes of the mother and father. DNA profiling is also used in criminal investigations.
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Below is a DNA profile of an individual. Use the diagram below and your knowledge from lecture to answer the questions below.
1. The single peak at D16S539 means that the individual has only one copy of D16S539.
2. The individual is homozygous for both the D5S818 and D7S820 loci.
3. The individual is heterozygous for the D16S539 locus.
4. The numbered boxes at the bottom represent the number of repeats.
D5S818
D13S317
D7S820
D16S539
CSFIPO
Number of Base Pairs
100
200
300
11 12
9 13
13|1ג|
11
10 11
A. 1, 2 and 3
B. 1 and 3
C. 2 and 4
D. 4 only
E. All of 1, 2, 3 and 4 are correct.
Relative
Fluorescence
You are a doctor who has obtained the karyotype below from a child to be born to one of your patients. Give your patient a brief description of karyotypes and explain how any abnormalities might have occurred.
An important application of DNA fingerprinting is relationship testing. Persons who are related genetically have some bands or peaks in common. The number they share depends on the closeness of their genetic relationship. For example, an offspring is expected to receive half of his or her minisatellites from one parent and the rest from the other. The diagram shown here schematically illustrates traditional DNA fingerprints of an offspring, mother, and two potential fathers. In paternity testing, the offspring’s DNA fingerprint is first compared with that of the mother. The bands that the offspring have in common with the mother are depicted in purple. The bands that are not similar between the offspring and the mother must have been inherited from the father. These bands are depicted in red. Which male could be the father?
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- Dideoxy sequencing is one of the most important methods for DNA sequencing. What could be the impact of variability on dideoxy sequencing? Hypothesize the same.arrow_forwardGive typing answer with explanation and conclusion Short tandem repeats (STRs) are used for DNA profiling. A STR with _____ (how many) alleles is a better marker to be used for DNA profiling?arrow_forwardDefine the following terms:a. A-DNAb. B-DNAc. Z-DNAd. cruciforme. palindromearrow_forward
- A researcher used polymerase chain reaction (PCR) to test four individual nematodes for the presence of a mutation in Gene Y. When amplified, the fragments of DNA containing the mutated gene alleles were 250 base pairs long and the normal (wild type) alleles were 350 base pairs long. To identify the genotypes of the individual nematodes, the researcher used gel electrophoresis to visualize the PCR results. 1.000 bp 500 bp 300 bp 200 bp 100 bp Remember that these nematodes are diploid organisms and as such, inherited two copies of Gene Y. Which of the following statements is a correct analysis of these results a) Nematode As homozygous for this gene and has two copies of the mutated allele. O b) Nematode C is heterozygous for this gene and has one copy of the mutated allele and one copy of the normal (wild type) allele. O) Nematode D is heterozygous for this gene and has one copy of the mutated allele and one copy of the normal (wild type) allele. ) d) Nematode B is homozygous for this…arrow_forwardThree newborn bables have been separated from their parents. You need to make sure that each child is reunited with the proper set of parents. The data below was gathered by amplifying two VNTR loci in each baby and the possible parents. Fragments were then separated using gel electrophoresis. Assume that each parent couple had only one of the babies. Match the babies with the proper parents. VNTR Locus 1 Baby 1 Baby 2 Baby 3 Jeff Jane David Dianna Mike Maddle VNTR Locus 2 Baby 1 Baby 2 Baby 3 Jeff Jane David Dianna Mike Maddie Baby y Choose... Mike and Maddie Baby David and Dianna Jeff and Jane Babyarrow_forwardMatching DNA samples in forensics uses a specific set of small “genes” called________ . The alleles of these genes in different people vary in the__________ of the allele. The pattern of these alleles that a given person possesses is called his or___________ her .arrow_forward
- Contrast the early DNA sequencing methods with those used today.arrow_forwardExplain if the laboratory is a good environment for PCR analysis. If not, explain what are the problems in the lab such as lack of cleanliness, etc.arrow_forwardWhich lab technique can you use to verify? a.Nucleic Acid Purification b. PCR c.SNP Variationarrow_forward
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