Biology (MindTap Course List)
10th Edition
ISBN: 9781285423586
Author: Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg
Publisher: Cengage Learning
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Chapter 8, Problem 14TYU
PREDICT Could the inner mitochondrial membrane carry out its functions in the coupling of electron transport and ATP synthesis if its lipid bilayer were readily permeable to hydrogen ions (protons)?
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Based on your results, which suspect's DNA best matches the DNA found at the crime scene?
Chapter 8 Solutions
Biology (MindTap Course List)
Ch. 8.1 - Write a summary reaction for aerobic respiration...Ch. 8.1 - Prob. 1CCh. 8.1 - Prob. 2CCh. 8.2 - Prob. 2LOCh. 8.2 - Prob. 3LOCh. 8.2 - Add up the energy captured (as ATP, NADH, and...Ch. 8.2 - Prob. 5LOCh. 8.2 - Prob. 6LOCh. 8.2 - Prob. 1CCh. 8.2 - Prob. 2C
Ch. 8.2 - What are the roles of NAD+, FAD, and oxygen in...Ch. 8.2 - Prob. 4CCh. 8.3 - Summarize how the products of protein and lipid...Ch. 8.3 - Prob. 1CCh. 8.3 - Prob. 2CCh. 8.3 - Prob. 3CCh. 8.4 - Compare and contrast anaerobic respiration and...Ch. 8.4 - What is the fate of hydrogen atoms removed from...Ch. 8.4 - What accounts for the ATP yield of fermentation...Ch. 8.4 - Is chemiosmosis involved in fermentation? in...Ch. 8 - A chemical process during which a substance gains...Ch. 8 - The reactions of _____ take place within the...Ch. 8 - Before pyruvate enters the citric acid cycle, it...Ch. 8 - In the first step of the citric acid cycle, an...Ch. 8 - Which of the following is the major source of...Ch. 8 - The aerobic part of aerobic cellular respiration...Ch. 8 - Prob. 7TYUCh. 8 - A net profit of only 2 ATPs can be produced...Ch. 8 - When deprived of oxygen, yeast cells obtain energy...Ch. 8 - Which of the following is a correct ranking of...Ch. 8 - Prob. 11TYUCh. 8 - CONNECT Explain why the proton gradient formed...Ch. 8 - CONNECT How are the endergonic reactions of the...Ch. 8 - PREDICT Could the inner mitochondrial membrane...Ch. 8 - Prob. 15TYUCh. 8 - Prob. 16TYUCh. 8 - EVOLUTION LINK The reactions of glycolysis are...Ch. 8 - Prob. 18TYU
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